Evaluation of a PCR kit for the detection of Erwinia carotovora subsp. atroseptica on potato tubers

Potato Research

Volumn 41, Issue 2, 1998, Pages 163-173

  Frechon, Dominique ^a   Exbrayat, Pascale ^a   Helias, Valerie ^b   Hyman, Lizbeth J ^c   Jouan, B ^b   Llop, P ^d   Lopez, Maria M ^d   Payet, Nicole ^a   Perombelon M C M ^c   Toth, I K ^c   Van Beckhoven, José R C M ^e   Van Der Wolf, J M ^e   Bertheau, Y ^a  

^a AGROPARISTECH   (France)

^b CEMAGREF   (France)

^c SCOTTISH CROP RESEARCH INSTITUTE   (United Kingdom)

^d INSTITUTO VALENCIANO DE INVESTIGACIONES AGRARIAS IVIA   (Spain)

^e WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Blackleg; DNA; ELISA

Indexed keywords

BACTERIUM DETECTION; CULTIVAR; DNA AMPLIFICATION; DNA PROBE; ENZYME LINKED IMMUNOSORBENT ASSAY; EXTRACTION; HYBRIDIZATION; INOCULATION; POLYMERASE CHAIN REACTION; POTATO TUBER; SPECIFICITY;

BACTERIA (MICROORGANISMS); ERWINIA; ERWINIA CAROTOVORA; PECTOBACTERIUM ATROSEPTICUM; PECTOBACTERIUM CAROTOVORUM; SOLANUM TUBEROSUM; VISCUM ALBUM;

EID: 7844227664     PISSN: 00143065     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02358439     Document Type: Article

References (21)

1. Aoki, Y. & H. Yamada, 1994. Clinical application of microplate DNA-DNA hybridization procedure for rapid diagnostics of Mycobacterial infections. Tubercle Lung Disease 75: 213-219.
2. Bain, R.A,. M.C.M. Pérombelon, L. Tsror & A. Nachmias, 1990. Blackleg development and tuber yield in relation to numbers of Erwinia carotovora subsp. atroseptica on seed potatoes. Plant Pathology 39: 125-133.
3. Bertheau, Y., D. Fréchon, L.J. Hyman & I.K. Toth, 1998. E. c. atroseptica DNA amplification by polymerase chain reaction (PCR). In: M.C.M. Pérombelon & J.M. van der Wolf (Eds), Methods for detection and quantification of Erwinia carotovora subsp. atroseptica on potatoes: a laboratory manual. Scottish Crop Research Institute Occasional Publication No. 10, Logomotives, Kirkcaldy, Scotland, UK, pp. 39-59.
4. Darrasse, A., A. Koutoujansky & Y. Bertheau, 1994. PCR and restriction fragment length polymorphism as a tool to identify Erwinia carotovora in relation to potato diseases. Applied and Environmental Microbiology 60: 1437-1443.
5. De Boer, S.H. & L.J. Ward, 1995. PCR detection of Erwinia carotovora subsp. atroseptica associated with potato tissue. Phytopathology 85: 854-858.
6. De Boer, S.H., L. Verdonck, H. Vruggink, P.Harju, H.O. Bang & J. DeLey, 1987. Serological and biochemical variation among potato strains of Erwinia carotovora subsp. atroseptica and their taxonomic relationship to other E. carotovora strains. Journal of Applied Bacteriology 63: 487-495.
7. Fréchon, D., P. Exbrayat, O. Gallet, E. Guillot, V. Le Clerc, N. Payet & Y. Bertheau, 1995. Séquences nucléotidiques pour la détection des Erwinia carotovora subsp. atroseptica. Brevet 95.12 803 INRA, INA P-G, SDP.
8. Gorris, M.T., B. Alarcon, M.M. Lopez & M. Cambra, 1994. Characterization of monoclonal antibodies specific for Erwinia carotovora subsp. atroseptica and comparison of serological methods for its sensitive detection on potato tubers. Applied and Environmental Microbiology 60: 2076-2085.
9. Hyman, L.J., V. Dewasmes, L.K. Toth & M.C.M. Pérombelon, 1997. Improved PCR detection sensitivity of Erwinia carotovora subsp. atroseptica in potato tuber peel extract by prior enrichment on a selective medium. Letters in Applied Microbiology 25: 143-147.
10. Hyman, L.J. & M.C.M. Pérombelon, 1990. Sensitivity of the ELISA method to detect potato seed contamination by Erwinia carotovora subsp. atroseptica below the threshold level for blackleg development. In: G,M. Williams (Ed.), Conference Proceedings Crop Protection in Northern Britain. William Culross & Son, Coupar Angus, Scotland, UK, pp. 219-224.
11. Hyman, L.J., A. Wallace, M.M. Lopez, M. Cambra, M.T. Gorris & M.C.M. Pérombelon, 1995. Characterization of monoclonal antibodies against Erwinia carotovora subsp. atroseptica serogroup I: specificity and epitope analysis. Journal of Applied Bacteriology 78: 437-444.
12. Jones, D.A.C., L.J. Hyman, M. Tumeseit, P. Smith & M.C.M. Pérombelon, 1994. Blackleg potential of potato seed: determination of tuber contamination by Erwinia carotovora subsp. atroseptica by immunofluorescence colony staining and stock and tuber sampling. Annals of Applied Biology 124: 557-568.
13. Palleroni, N.J., 1993. Structure of the bacterial genome. In: A. Goodfellow & A.G. O'Donnell (Eds), Handbook of new bacterial systematics. Academic Press, New York, USA, pp. 57-115.
14. Pérombelon, M.C.M., 1992. Potato blackleg: epidemiology, host-pathogen interaction and control. Netherlands Journal of Plant Pathology 98: 274-280.
15. Pérombelon, M.C.M. &. L.J. Hyman, 1986. A rapid method to identify and quantify soft rot erwinias directly from plant material based on their temperature tolerances and sensitivity to erythromycin. Journal of Applied Bacteriology 60: 61-66.
16. Pérombelon, M.C.M. & L.J. Hyman, 1995. Serological methods to quantify potato seed contamination by Erwinia carotovora subsp. atroseptica. EPPO Bulletin 25: 195-202.
17. Sambrooke, J., E.F. Fritsch & T. Maniatis, 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring, N.Y., USA.
18. Seal, S.E., 1996. Molecular diagnosis of plant pathogenic prokaryotes in the tropics. In: Brighton Crop Protection Conference, Conference Proceedings vol. 2. British Crop Protection Council, UK, pp. 561-570.
19. Smid, E.J., A.H.J. Jansen & L.G.M. Gorris, 1995. Detection of Erwinia carotovora. subsp. atroseptica and Erwinia chrysanthemi in potato tubers using polymerase chain reaction. Plant Pathology 44: 1058-1069.
20. Wing, R.A., J. Yamaguchi, S.K. Larabell, V.M. Ursin & S. McCormack, 1989. Molecular and genetic characterization of two pollen-expressed genes that have sequence similarity to pectate lyases of the pathogen Erwinia. Plant Molecular Biology 14:17-28.
21. Wolf, J.M. van der, L.J. Hyman, D.A.C. Jones, C. Grévesse, J.R.C.M. van Beckhoven, J.W.L. van Vuurde & M.C.M. Pérombelon, 1996. Immunomagnetic separation of Erwinia carotovora subsp. atroseptica from potato peel extracts to improve detection sensitivity on crystal violet pectate medium or by PCR. Journal of Applied Bacteriology 80: 487-495.