Rapid species identification within two groups of closely related lactobacilli using PCR primers that target the 16S/23S rRNA spacer region

FEMS Microbiology Letters

Volumn 161, Issue 1, 1998, Pages 97-106

  Berthier, Françoise ^a,b   Ehrlich, Stanislav Dusko ^a  

^a CEMAGREF   (France)

^b SRTAL   (France)

Author keywords

16S 23S rRNA spacer region; L. curvatus; L. graminis; L. paraplantarum; L. pentosus; L. plantarum; L. sake; Polymerase chain reaction; Species specific probe

Indexed keywords

BACTERIAL RNA; RIBOSOME RNA;

ARTICLE; BACTERIUM IDENTIFICATION; DNA DNA HYBRIDIZATION; LACTOBACILLUS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA ANALYSIS; RNA SEQUENCE; SEQUENCE ANALYSIS; STRAIN DIFFERENCE;

BASE SEQUENCE; LACTOBACILLUS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 23S; SENSITIVITY AND SPECIFICITY;

EID: 0032054366     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00055-X     Document Type: Article

References (19)

1. Fox, G.E., Wisotzkey, J.D. and Jurtshuk, Jr., P. (1992) How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. Int. J. Syst. Bacteriol. 42, 166-170.
2. Schleifer, K.-H., Ehrmann, M., Beimfohr, C., Brockmann, E., Ludwig, W. and Amann, R. (1995) Application of molecular methods for the classification and identification of lactic acid bacteria. Int. Dairy J. 5, 1081-1094.
3. Curk, M.-C., Hubert, J.-C. and Bringel, F. (1996) Lactobacillus paraplantarum sp.nov., a new species related to Lactobacillus plantarum. Int. J. Syst. Bacteriol. 46, 595-598.
4. Bringel, F., Curk, M.-C. and Hubert, J.-C. (1996) Characterization of lactobacilli by Southern-type hybridization with a Lactobacillus plantarum pyrDFE probe. Int. J. Syst. Bacteriol. 46, 588-594.
5. Moreira, D. and Amils, R. (1996) PCR-mediated detection of the chemolithotrophic bacterium Thiobacillus cuprinus using 23S rDNA- and 16S/23S intergenic spacer region-targeted oligonucleotide primers. FEMS Microbiol. Lett. 142, 289-293.
6. Graham, T., Goldsteyn-Thomas, E.J., Gannon, V.P.J. and Thomas, J.E. (1996) Genus- and species-specific detection of Listeria monocytogenes using polymerase chain reaction assays targeting the 16S-23S intergenic spacer region of the rRNA operon. Can. J. Microbiol. 42, 1155-1162.
7. Gürtler, V. and Stanisich, V.A. (1996) New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology 142, 3-16.
8. Nagakawa, T., Shimada, M., Mukai, H., Asada, K., Kato, I., Fujino, K. and Sato, T. (1994) Detection of alcohol-tolerant hiochi bacteria by PCR. Appl. Environ. Microbiol. 60, 637-640.
9. Morishita, Y. and Shiromizu, K. (1986) Characterization of lactobacilli isolated from meats and meat products. Int. J. Food Microbiol. 3, 19-29.
10. Montel, M.-C., Talon, R., Fournaud, J. and Champomier, M.-C. (1991) A simplified key for identifying homofermentative Lactobacillus and Carnobacterium spp. from meat. J. Appl. Bacteriol. 70, 469-472.
11. de Man, J.C., Rogosa, M. and Sharpe, M.E. (1960) A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23, 130-135.
12. De Los Reyes-Gavilan, C.G., Limsowtin, G.K.Y., Tailliez, P., Séchaud, L. and Accolas, J.-P. (1992) A Lactobacillus helveticus-specific DNA probe detects restriction fragment length polymorphisms in this species. Appl. Environ. Microbiol. 58, 3429-3432.
13. Kagermaier-Callaway, A.S. and Lauer, E. (1995) Lactobacillus sake Katagari, Kitahara, and Fukama 1934 is the senior synonym for Lactobacillus bavaricus Stetter and Stetter 1980. Int. J. Syst. Bacteriol. 45, 398-399.
14. Loughney, K., Lund, E. and Dahlberg, J.E. (1982) tRNA genes are found between the 16S and the 23S rRNA genes in Bacillus subtilis. Nucleic Acids Res. 10, 1607-1624.
15. Nour, M., Naimi, A., Beck, G. and Branlant, C. (1995) 16S-23S and 23S-5S intergenic spacer regions of Streptococcus thermophilus and Streptococcus salivarius, primary and secondary structure. Curr. Microbiol. 31, 270-270.
16. Collins, M.D., Rodrigues, U., Ash, C., Aguirre, M., Farrow, J.A.E., Martinez-Murcia, A., Phillips, B.A., Williams, A.M. and Wallbanks, S. (1991) Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reversed transcriptase sequencing of 16S rRNA. FEMS Microbiol. Lett. 77, 5-12.
17. Rijpens, N.P., Jannes, G., Van Asbrek, M., Herman, L.M.F. and Rossau, R. (1995) Simultaneous detection of Listeria spp. and Listeria monocytogenes by reverse hybridization with 16S-23S rRNA probes. Mol. Cell. Probes 9, 423-432.
18. Hertel, C., Ludwig, W., Obst, M., Vogel, R.F., Hammes, W. and Schleifer, K.H. (1991) 23S rRNA-targeted oligonucleotide probe for the rapid identification of meat lactobacilli. Syst. Appl. Microbiol. 14, 173-177.
19. Rijpens, N.P., Jannes, G., Van Asbroek, M., Rossau, R. and Herman, L.M.F. (1996) Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes. Appl. Environ. Microbiol. 62, 1683-1688.