Hepatitis C virus nonstructural proteins are localized in a modified endoplasmic reticulum of cells expressing viral subgenomic replicons

Virology

Volumn 293, Issue 1, 2002, Pages 31-43

  Mottola, Giovanna ^b   Cardinali, Giorgia ^c   Ceccacci, Alessandra ^a   Trozzi, Caterina ^a   Bartholomew, Linda ^a   Torrisi, Maria Rosaria ^c,d   Pedrazzini, Emanuela ^e   Bonatti, Stefano ^b   Migliaccio, Giovanni ^a  

^a IRBM   (Italy)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^c SAN GALLICANO DERMATOLOGICAL INSTITUTE   (Italy)

^d SAPIENZA UNIVERSITY OF ROME   (Italy)

^e UNIVERSITY OF MILAN   (Italy)

Author keywords

Endoplasmic reticulum; HCV; Hepatitis C virus; Nonstructural protein; Replication complex

Indexed keywords

VIRUS PROTEIN;

ARTICLE; BIOMICROSCOPY; CELL FRACTIONATION; CELL STRUCTURE; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; IMMUNOELECTRON MICROSCOPY; IN VITRO STUDY; LIVER BIOPSY; LIVER MITOCHONDRION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; REPLICON; RNA REPLICATION; TISSUE CULTURE CELL; VIRUS REPLICATION;

FLAVIVIRUS; HEPATITIS C VIRUS;

EID: 0036346236     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.2001.1229     Document Type: Article

References (50)

1. Hepatocellular mitochondrial alterations in patients with chronic hepatitis C: Ultrastructural and biochemical findings
2. Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus
3. Autocrine motility factor receptor is a marker for a distinct membranous tubular organelle
4. Efficient initiation of HCV RNA replication in cell culture
5. Experimental infection of chimpanzees with antihemophilic (factor VIII) materials: Recovery of virus-like particles associated with non-A, non-B hepatitis
6. Viral glycoproteins accumulate in newly formed annulate lamellae following infection of lymphoid cells by human herpesvirus 6
7. Isolation of a cDNA clone derived a blood-borne non-A non-B viral hepatitis genome
8. Genetic organization and diversity of the hepatitis C virus
9. Biochemical and immunologic properties of the nonstructural proteins of the hepatitis C virus: Implications for development of antiviral agents and vaccines
10. In vitro infection of adult normal human hepatocytes in primary culture by hepatitis C virus
11. Suppression of actinomycin D-induced apoptosis by the NS3 protein of hepatitis C virus
12. Preparation of monoclonal antibodies: Strategies and procedures
13. Modulation of hepatitis C virus NS3 protease and helicase activities through the interaction with NS4A
14. Human hepatitis C virus NS5A protein alters intracellular calcium levels, induces oxidative stress, and activates STAT-3 and NF-κB
15. 2+ signaling between sarco-endoplasmic reticulum and mitochondria
16. Hepatitis C viruses
17. Hepatitis C virus NS5B protein is a membrane-associated phosphoprotein with a predominantly perinuclear localization
18. Characterization of the nuclear localization signal and subcellular distribution of hepatitis C virus nonstructural protein NS5A
19. Human hepatocyte clonal cell lines that support persistent replication of hepatitis C virus
20. Cultivation of hepatitis C virus in primary hepatocyte culture from patients with chronic hepatitis C results in release of high titre infectious virus
21. The natural course of chronic hepatitis
22. Molecular cloning of human hepatitis C virus genome from Japanese patients with non-A non-B hepatitis
23. Hepatitis C virus and hepatocellular carcinoma
24. Transmission of hepatitis C by intrahepatic inoculation with transcribed RNA
25. Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT/PCR
26. Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line
27. The signal sequence receptor, unlike the signal recognition particle receptor, is not required for protein translocation
28. Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups
29. Calreticulin, and not calsequestrin, is the major calcium binding protein of smooth muscle sarcoplasmic reticulum and liver endoplasmic reticulum
30. Characterization of hepatitis C virus replication in cloned cells obtained from a human T-cell leukemia virus type 1-infected cell line, MT-2
31. A new determinant of endoplasmic reticulum localization is contained in the juxtamembrane region of the ectodomain of hepatitis C virus glycoprotein E1
32. Characterization of cell lines carrying self-replicating hepatitis C virus RNAs
33. 2+ influx in bcl-2-overexpressing cells
34. Overview of hepatitis C virus genome structure, polyprotein processing, and protein properties
35. Flaviviridae: The viruses and their replication
36. Mitochondria as all-round players of the calcium games
37. 2+ revealed by specifically targeted recombinant aequorin
38. Intracytoplasmic crystalline inclusions in the hepatocytes of humans and chimpanzees
39. Ultrastructure of normal and hepatitis virus infected human and chimpanzee liver: Similarities and differences
40. Correlation between the infectivity of hepatitis C virus in vivo and its infectivity in vitro
41. Structure and organization of the hepatitis C virus genome isolated from human carriers
42. NS3 is a serine protease required for processing of hepatitis C virus polyprotein
43. Localization of Epstein-Barr virus envelope glycoproteins on the inner nuclear membrane of virus-producing cells
44. Calcium regulates the association between mitochondria and a smooth subdomain of the endoplasmic reticulum
45. Epidemiology of hepatitis C: Geographic differences and temporal trends
46. Ultrastructure of Kunjin virus-infected cells: Colocalization of NS1 and NS3 with double-stranded RNA, and of NS2B with NS3, in virus-induced membrane structures
47. Subcellular localization, stability, and trans-cleavage competence of the hepatitis C virus NS3-NS4A complex expressed in tetracycline-regulated cell lines
48. Transcripts from a single full-length cDNA clone of hepatitis C virus are infectious when transfected into the liver of a chimpanzee
49. Transcripts of a chimeric cDNA clone of hepatitis C virus genotype 1b are infectious in vivo