Cytomegalovirus assemblin (pUL80a): Cleavage at internal site not essential for virus growth; proteinase absent from virions

Journal of Virology

Volumn 76, Issue 17, 2002, Pages 8667-8674

  Chan, Chee Kai ^a   Brignole, Edward J ^a   Gibson, Wade ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASSEMBLIN; PROTEINASE;

ARTICLE; GENETIC TRANSFECTION; HUMAN CYTOMEGALOVIRUS; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; VIRION; VIROGENESIS; VIRUS GENOME; VIRUS INFECTION; VIRUS MUTANT; VIRUS PATHOGENESIS;

CELL LINE; CHROMOSOMES, ARTIFICIAL, BACTERIAL; CYTOMEGALOVIRUS; CYTOPATHOGENIC EFFECT, VIRAL; ENDOPEPTIDASES; HUMANS; MUTATION; SERINE ENDOPEPTIDASES; TRANSFECTION; VIRION; VIRUS ASSEMBLY;

EID: 0036337843     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.76.17.8667-8674.2002     Document Type: Article

References (56)

1. Expression and analysis of the human cytomegalovirus UL80-encoded protease: Identification of autoproteolytic sites
2. Virus-specific interaction between the human cytomegalovirus major capsid protein and the C terminus of the assembly protein precursor
3. Cloning of the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome in Escherichia coli: A new approach for construction of HCMV mutants
4. Genetic evidence of an essential role for cytomegalovirus small capsid protein in viral growth
5. Human cytomegalovirus maturational proteinase: Expression in Escherichia coli, purification, and enzymatic characterization by using peptide substrate mimics of natural cleavage sites
6. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169
7. Structure of the human cytomegalovirus protease catalytic domain reveals a novel serine protease fold and catalytic triad
8. Characterization of human cytomegalovirus protease dimerization by analytical centrifugation
9. Active human cytomegalovirus protease is a dimer
10. Identification of genes encoding two capsid proteins (VP24 and VP26) of herpes simplex virus type 1
11. Herpes simplex virus type 1 VP26 is not essential for replication in cell culture but influences production of infectious virus in the nervous system of infected mice
12. Identification of the herpes simplex virus type 1 protease cleavage sites by direct sequence analysis of autoproteolytic cleavage products
13. RNase P-mediated inhibition of cytomegalovirus protease expression and viral DNA encapsidation by oligonucleotide external guide sequences
14. Electrophoretic analysis of the major polypeptides of the human euthrocyte membrane
15. Protein counterparts of human and simian cytomegaloviruses
16. Structural and nonstructural proteins of strain Colburn cytomegalovirus
17. Structure and assembly of the virion
18. Cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus UL46
19. Proteins specified by herpes simplex virus. VIII. Characterization and composition of multiple capsid forms of subtypes 1 and 2
20. Assemblin, a herpes virus serine maturational proteinase and new molecular target for antivirals
21. Assemblin homolog of herpes simplex virus type 1 retains proteolytic activity when expressed as a recombinant two-chain enzyme
22. Cytomegalovirus assemblin: The amino and carboxyl domains of the proteinase form active enzyme when separately cloned and coexpressed in eukaryotic cells
23. c, can restore proteolytic activity to assemblin mutants by intermolecular complementation
24. Herpesvirus proteases: Targets for novel antiviral drugs
25. Activity of two-chain recombinant human cytomegalovirus protease
26. Isolation and characterization of a non-infectious virion-like particle released from cells infected with human strains of cytomegalovirus
27. Isolation of human cytomegalovirus intranuclear capsids, characterization of their protein constituents, and demonstration that the B-capsid assembly protein is also abundant in noninfectious enveloped particles
28. Proteolytic activity of human cytomegalovirus UL80 proteinase cleavage site mutants
29. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
30. Characterization of the protease and other products of amino-terminus-proximal cleavage of the herpes simplex virus 1 UL26 protein
31. The herpes simplex virus 1 gene encoding a protease also contains within its coding domain the gene encoding the more abundant substrate
32. Dimerization of the human cytomegalovirus protease: Kinetic and biochemical characterization of the catalytic homodimer
33. Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome
34. Cytomegalovirus bacterial artificial chromosomes: A new herpesvirus vector approach
35. Structure of the herpes simplex virus capsid: Effects of extraction with guanidine hydrochloride and partial reconstitution of extracted capsids
36. The effect of internal autocleavage on kinetic properties of the human cytomegalovirus protease catalytic domain
37. Self-association of herpes simplex virus type 1 ICP35 is via coiled-coil inter-actions and promotes stable interaction with the major capsid protein
38. Single-chain recombinant human cytomegalovirus protease. Activity against its natural protein substrate and fluorogenic peptide substrates
39. Cytomegalovirus assembly protein precursor and proteinase precursor contain two nuclear localization signals that mediate their own nuclear translocation and that of the major capsid protein
40. Auto-inactivation by cleavage within the dimer interface of Kaposi's sarcoma-associated herpesvirus protease
41. Identification and characterization of a herpes simplex virus gene product required for encapsidation of virus DNA
42. Processing of the herpes simplex virus assembly protein ICP35 near its carboxy-terminal end requires the product of the whole of the UL26 reading frame
43. Unique fold and active site in cytomegalovirus protease
44. Peptide substrate cleavage specificity of the human cytomegalovirus protease
45. Evidence for controlled incorporation of herpes simplex virus type 1 UL26 protease into capsids
46. Three-dimensional structure of human cytomegalovirus protease
47. Chelating peptide immobilized metal ion affinity chromatography. A new concept in affinity chromatography for recombinant proteins
48. Purification and kinetic characterization of human cytomegalovirus assemblin
49. Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion
50. In vitro proteolytic activity and active-site identification of the human cytomegalovirus proteinase
51. A new serine-protease fold revealed by the crystal structure of human cytomegalovirus protease
52. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
53. The herpesvirus proteases as targets for antiviral chemotherapy
54. Herpesvirus proteinase: Site-directed mutagenesis used to study maturational, release, and inactivation cleavage sites of precursor and to identify a possible catalytic site serine and histidine
55. A herpesvirus maturational protease, assemblin: Identification of its gene, putative active site domain, and cleavage site
56. Human cytomegalovirus capsid assembly protein precursor (pUL80.5) interacts with itself and with the major capsid protein (pUL86) through two different domains