Cytomegalovirus assemblin: The amino and carboxyl domains of the proteinase form active enzyme when separately cloned and coexpressed in eukaryotic cells

Journal of Virology

Volumn 70, Issue 8, 1996, Pages 5395-5404

  Hall, Matthew R T ^a   Gibson, Wade ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; CYTOMEGALOVIRUS; ENZYME SPECIFICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DEGRADATION; VIRUS ASSEMBLY;

EID: 0029943395     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.8.5395-5404.1996     Document Type: Article

References (51)

1. Babé, L. M., J. Rose, and C. S. Craik. 1992. Synthetic interface peptides alter dimeric assembly of the HIV-1 and HIV-2 proteases. Protein Sci. 1:1244-1253.
2. Baum, E. Z., G. A. Bebernitz, J. D. Hulmes, V. P. Muzithras, T. R. Jones, and Y. Gluzman. 1993. Expression and analysis of the human cytomegalovirus UL80-encoded protease: identification of autoproteolytic sites. J. Virol. 67:497-506.
3. Burck, P. J., D. H. Berg, T. P. Luk, L. M. Sassmannshausen, M. Wakulchik, G. W. Becker, D. P. Smith, H. M. Hsiung, W. Gibson, and E. C. Villarreal. 1994. Human cytomegalovirus proteinase: purification of the enzyme and determination of its activity by using peptide substrates that mimic its native cleavage sites. J. Virol. 68:2937-2946.
4. Carrascosa, A. L. 1994. Enhancement of baculovirus plaque assay in insect cell monolayers by DEAE-dextran. BioTechniques 16:1078-1081, 1083-1085.
5. Cerretti, D. P., C. J. Kozlosky, B. Mosley, N. Nelson, N. K. Van, T. A. Greenstreet, C. J. March, S. R. Kronheim, T. Druck, L. A. Cannizzaro, K. Huebner, and R. A. Black. 1992. Molecular cloning of interleukin-I beta converting enzyme. Science 256:97-100.
6. Chen, C., and H. Okayama. 1987. High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7:2745-2752.
7. Cox, G. A., M. Wakulchik, L. M. Sassmannshausen, W. Gibson, and E. C. Villarreal. 1995. Human cytomegalovirus proteinase: candidate glutamic acid identified as third member of putative active site triad. J. Virol. 69: 4524-4528.
8. Dilanni, C. L., D. A. Drier, I. C. Deckman, P. J. McCann, F. Liu, B. Roizman, R. J. Colonno, and M. G. Cordingley. 1993. Identification of the herpes simplex virus-1 protease cleavage sites by direct sequence analysis of autoproteolytic cleavage products. J. Biol. Chem. 268:2048-2051.
9. Dilanni, C. L., J. T. Stevens, M. Bolgar, D. R. O'Boyle, S. P. Weinheimer, and R. J. Colonno. 1994. Identification of the serine residue at the active site of the herpes simplex virus type I protease. J. Biol. Chem. 269:12672-12676.
10. Gao, M., L. Matusick-Kumar, W. Hurlburt, S. F. DiTusa, W. W. Newcomb, J. C. Brown, P. J. McCann, I. Deckman, and R. J. Colonno. 1994. The protease of herpes simplex virus type 1 is essential for functional capsid formation and viral growth. J. Virol. 68:3702-3712.
11. Gibson, W. 1981. Structural and non structural proteins of strain Colburn cytomegalovirus. Virology 111:516-537.
12. Gibson, W., A. R. Welch, and M. R. T. Hall. 1995. Assemblin, a herpes virus serine maturational proteinase and new molecular target for antivirals. Perspect. Drug Discovery Design 2:413-426.
13. Gibson, W., A. R. Welch, and J. Ludford. 1994. Transient transfection assay of the herpesvirus maturational proteinase, assemblin. Methods Enzymol. 244:399-411.
14. Hall, D. L., and P. L. Darke. 1995. Activation of the herpes simplex virus type 1 protease. J. Biol. Chem. 270:22697-22700.
15. Holwerda, B. C., A. J. Wittwer, K. L. Duffin, C. Smith, M. V. Toth, L. S. Carr, R. C. Wiegand, and M. L. Bryant. 1994. Activity of two-chain recombinant human cytomegalovirus protease. J. Biol. Chem. 269:25911-25915.
16. Jones, T. R., L. Sun, G. A. Bebernitz, V. P. Muzithras, H.-J. Kim, S. H. Johnston, and E. Z. Baum. 1994. Proteolytic activity of human cytomegalovirus UL80 proteinase cleavage site mutants. J. Virol. 68:3742-3752.
17. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
18. 125I using intensifying screens and hypersensitized film. FEBS Lett. 82:314-316.
19. Liu, F., and B. Roizman. 1991. The promoter, transcriptional unit, and coding sequence of herpes simplex virus 1 family 35 proteins are contained within and in frame with the UL26 open reading frame. J. Virol. 65:206-212.
20. Liu, F., and B. Roizman. 1991. The herpes simplex virus 1 gene encoding a protease also contains within its coding domain the gene encoding the more abundant substrate. J. Virol. 65:5149-5156.
21. Long, E. O., S. Rosen-Bronson, D. R. Karp, M. Malnati, R. P. Sekaly, and D. Jaraquemada. 1991. Efficient cDNA expression vectors for stable and transient expression of HLA-DR in transfected fibroblast and lymphoid cells. Hum. Immunol. 31:229-235.
22. Loutsch, J. M., N. J. Galvin, M. L. Bryant, and B. C. Holwerda. 1994. Cloning and sequence analysis of murine cytomegalovirus protease and capsid assembly protein genes. Biochem. Biophys. Res. Commun. 203:472-478.
23. Mullis, K. B., F. Faloona, S. Scharf, R. Saiki, G. Horn, and E. Erlich. 1986. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symp. Quant. Biol. 51:263-273.
24. O'Boyle, D. R., K. Wager-Smith, J. T. Stevens, and S. P. Weinheimer. 1995. The effect of internal autocleavage on kinetic properties of the human cytomegalovirus protease catalytic domain. J. Biol. Chem. 270:4753-4758.
25. O'Reilly, D. R., L. K. Miller, and V. A. Lucklow. 1992. Baculovirus expression vectors: a laboratory manual. W. H. Freeman and Company, New York.
26. Ponting, C. P., J. M. Marshall, and S. A. Cederhom-Williams. 1992. Plasminogen: a structural review. Blood Coagul, Fibrinolysis 3:605-614.
27. Preston, V. G., J. A. Coates, and F. J. Rixon. 1983. Identification and characterization of a herpes simplex virus gene product required for encapsidation of virus DNA. J. Virol. 45:1056-1064.
28. Preston, V. G., F. J. Rixon, I. M. McDougall, M. McGregor, and M. F. Al Kobaisi. 1992. Processing of the herpes simplex virus assembly protein ICP35 near its carboxy terminal end requires the product of the whole of the UL26 reading frame. Virology 186:87-98.
29. Ramage, P., D. Cheneval, M. Chvei, P. Graff, R. Hemmig, R. Heng, H. P. Kocher, A. Mackenzie, K. Memmer, L. Revesz, and W. Wishart. 1995. Expression, refolding, and autocatalytic processing of the interleukin-1 beta converting enzyme precursor. J. Biol. Chem. 270:9378-9383.
30. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
31. Sardana, V. V., J. A. Wolfgang, C. A. Veloski, W. J. Long, K. LeGrow, B. Wolanski, E. A. Emini, and R. L. LaFemina. 1994. Peptide substrate cleavage specificity of the human cytomegalovirus protease. J. Biol. Chem. 269: 14337-14341.
32. Schechter, I., and A. Berger. 1967. On the size of the active site in proteases. I. Papain. Biochem. Biophys. Res. Commun. 27:157-162.
33. Schenk, P., A. S. Woods, and W. Gibson. 1991. The 45-kilodalton protein of cytomegalovirus (Colburn) B-capsids is an amino-terminal extension form of the assembly protein. J. Virol. 65:1525-1529.
34. Smith, M. C., T. C. Furman, T. D. Ingolia, and C. Pidgeon. 1988. Chelating peptide immobilized metal ion affinity chromatography. A new concept in affinity chromatography for recombinant proteins. J. Biol. Chem. 263:7211-7215.
35. Smith, M. C., J. Giordano, J. A. Cook, M. Wakulchik, E. C. Villarreal, G. W. Becker, K. Bemis, J. Labus, and J. S. Manetta. 1994. Purification and kinetic characterization of human cytomegalovirus assemblin. Methods Enzymol. 244:412-423.
36. Stevens, J. T., C. Mapelli, J. Tsao, M. Hail, D. O'Boyle, S. P. Weinheimer, and C. L. Dilanni. 1994. In vitro proteolytic activity and active-site identification of the human cytomegalovirus proteinase. Eur. J. Biochem. 226:361-367.
37. Stubbs, M. T., and W. Bode. 1994. Coagulation factors and their inhibitors. Curr. Opin. Struct. Biol. 4:823-832.
38. Summers, M. D., and G. E. Smith. 1987. A manual of methods for baculovirus vectors and insect cell culture procedures, Texas Agricultural Experiment Station, College Station.
39. Tate, K. M., D. L. Higgins, W. E. Holmes, M. E. Winkler, H. L. Heyneker, and G. A. Vehar. 1987. Functional role of proteolytic cleavage at arginine-275 of human plasminogen activator as assessed by site-directed mutagenesis. Biochemistry 26:338-343.
40. Taylor, J. W., J. Ott, and F. Eckstein. 1985. The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA. Nucleic Acids Res. 13:8765-8785.
41. Taylor, J. W., W. Schmidt, R. Cosstick, A. Okruszek, and F. Eckstein. 1985. The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA. Nucleic Acids Res. 13:8749-8764.
42. Thornberry, N. A., H. G. Bull, J. R. Calaycay, K. T. Chapman, A. D. Howard, M. J. Kostura, D. K. Miller, S. M. Molineaux, J. R. Weidner, J. Aunins, K. O. Elliston, J. M. Ayala, F. J. Cassano, J. Chin, G. J.-F. Ding, L. A. Egger, E. P. Gaffney, G. Limjuco, O. C. Palyha, S. M. Raju, A. M. Rolando, J. P. Salley, T.-t. Yamin, T. D. Lee, J. E. Shively, M. MacCross, R. A. Mumford, J. A. Schmidt, and M. J. Tocci. 1992. A novel heterodimeric cysteine proteinase is required for interleukin-1 beta processing in monocytes. Nature (London) 356:768-774.
43. Thornberry, N. A., and S. M. Molineaux. 1995. Interleukin-1 beta converting enzyme: a novel cysteine protease required for IL-I beta production and implicated in programmed cell death. Protein Sci. 4:3-12.
44. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
45. 2 homodimer. Cell 78:343-352.
46. Welch, A. R., L. M. McNally, and W. Gibson. 1991. Cytomegalovirus assembly protein nested gene family: four 3′-coterminal transcripts encode four in-frame, overlapping proteins. J. Virol. 65:4091-4100.
47. Welch, A. R., L. M. McNally, M. R. T. Hall, and W. Gibson. 1993. Herpesvirus proteinase: site-directed mutagenesis used to study maturational, release, and inactivation cleavage sites of precursor and to identify a possible catalytic site serine and histidine. J. Virol. 67:7360-7372.
48. Welch, A. R., E. C. Villarreal, and W. Gibson. 1995. Human cytomegalovirus (HCMV) and herpes simplex virus type 1 (HSV-1) homologs of the simian CMV proteinase assemblin are active, but the HSV-I enzyme shows a more restricted substrate specificity then the CMV enzymes. J. Virol. 69:341-347.
49. Welch, A. R., A. S. Woods, L. M. McNally, R. J. Cotter, and W. Gibson. 1991. A herpesvirus maturational protease. assemblin: identification of its gene, putative active site domain, and cleavage site. Proc. Natl. Acad. Sci. USA 88:10792-10796.
50. Wilson, K. P., J. A. Black, J. A. Thomson, E. E. Kim, J. P. Griffith, M. A. Navia, M. A. Murcko, S. P. Chambers, R. A. Aldape, S. A. Raybuck, and D. J. Livingston. 1994. Structure and mechanism of interleukin-1 beta converting enzyme. Nature (London) 370:270-275.
51. Yamanaka, G. W., C. L. Dilanni, D. R. O'Boyle II, J. Stevens, S. P. Weinheimer, I. C. Deckman, L. Matusick-Kumar, and R. J. Colonno. 1995. Stimulation of the herpes simplex virus type I protease by antichaeotrophic salts. J. Biol. Chem. 270:30168-30172.