The intercalating β-hairpin of T7 RNA polymerase plays a role in promoter DNA melting and in stabilizing the melted DNA for efficient RNA synthesis

Journal of Molecular Biology

Volumn 315, Issue 5, 2002, Pages 1009-1025

  Stano, Natalie M ^a   Patel, Smita S ^a  

^a RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

Author keywords

2 aminopurine; Fluorescence stopped flow; Open complex; Transcription initiation

Indexed keywords

DNA; ESTER DERIVATIVE; RNA; RNA POLYMERASE;

ARTICLE; BACTERIOPHAGE T7; CHEMICAL BOND; COMPLEX FORMATION; DNA DENATURATION; DNA STRAND; DNA TEMPLATE; GENE MUTATION; INTERCALATION COMPLEX; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; PROTEIN FUNCTION; PROTEIN STABILITY; RNA SYNTHESIS;

EID: 0036308760     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.5313     Document Type: Article

References (29)

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16. 2-Aminopurine fluorescence quenching and lifetimes: Role of base stacking
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18. Fluorescence studies of nucleotides and polynucleotides. I. Formycin, 2-aminopurine riboside, 2,6-diaminopurine riboside, and their derivatives
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21. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
22. Transcription by T7 RNA polymerase is not zinc-dependent and is abolished on amido-methylation of cysteine-347
23. A double-filter method for nitrocellulose-filter binding: Application to protein-nucleic acid interactions
24. Protein-nucleic acid interactions during open complex formation investigated by systematic alteration of the protein and DNA binding partners
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26. Aromatic amino acids in region 2.3 of Escherichia coli sigma 70 participate collectively in the formation of an RNA polymerase-promoter open complex
27. Different roles for basic and aromatic amino acids in conserved region 2 of Escherichia coli σ70 in the nucleation and maintenance of the single-stranded DNA bubble in open RNA polymerase-promoter complexes
28. Intercalation, DNA kinking, and the control of transcription
29. Transient-state kinetic analysis of enzyme reaction pathways