Aromatic amino acids in region 2.3 of Escherichia coli sigma 70 participate collectively in the formation of an RNA polymerase-Promoter open complex

Journal of Molecular Biology

Volumn 299, Issue 5, 2000, Pages 1217-1230

  Panaghie, Gianina ^a   Aiyar, Sarah E ^a   Bobb, Kathryn L ^a   Hayward, Richard S ^b   De Haseth, Pieter L ^a  

^a CASE WESTERN RESERVE UNIVERSITY   (United States)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Closed complex; Open complex; Promoter; RNA polymerase; Strand separation

Indexed keywords

AROMATIC AMINO ACID; DNA; NUCLEOSIDE TRIPHOSPHATE; RNA; RNA POLYMERASE;

AMINO ACID SUBSTITUTION; ARTICLE; COLD SENSITIVITY; COMPLEX FORMATION; ESCHERICHIA COLI; GENE CONTROL; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; TRANSCRIPTION INITIATION;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 0034705333     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3808     Document Type: Article

References (42)

1. Modification of the megaprimer method of PCR mutagenesis: Improved amplification of the final product
2. Mutations in sigma factor that affect the temperature dependence of transcription from a promoter, but not from a mismatch bubble in double-stranded DNA
3. Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA
4. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding
5. Conserved structures and diversity of functions of RNA-binding proteins
6. A procedure for the rapid, large scale preparation of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatography
7. 70 subunit from Escherichia coli RNA polymerase are modulated by an interaction with the core enzyme
8. DNA footprints of the two kinetically significant intermediates in formation of an RNA polymerase-promoter open complex: Evidence that interactions with the start site and downstream DNA induce sequential conformational changes m polymerase and DNA
9. Open complex formation by Escherichia coli RNA polymerase: The mechanism of polymerase-induced strand separation of double helical DNA
10. RNA polymerase-promoter interaction: The comings and goings of RNA polymerase
11. Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters
12. Equilibrium and kinetic parameters of the sequence-specific interaction of Escherichia coli RNA polymerase with nontemplate strand oligodeoxyribonucleotides
13. Escherichia coli promoter opening and -10 recognition: Mutational analysis of σ70
14. A simple procedure for resolution of Escherichia coli RNA polymerase holoenzyme from core polymerase
15. 70 abrogates productive transcription and restores expected mobility on a denaturing gel
16. Kinetic analysis of RNA polymerase-promoter interactions
17. Mechanism of activation of transcription initiation from the lambda P(RM) promoter
18. Structure and function of bacterial sigma factors
19. Protein-nucleic acid interactions during open complex formation investigated by systematic alteration of the protein and DNA binding partners
20. RNA polymerase bound to the P(R) promoter of bacteriophage lambda inhibits open complex formation at the divergently transcribed P(RM) promoter: Implications for an indirect mechanism of transcriptional activation by lambda repressor
21. 70 subunit of Escherichia coli RNA polymerase arid the nontemplate strand oligonucleotide. Luminescence resonance energy transfer study
22. Sigma factor mutations affecting the sequence-selective interaction of RNA polymerase with -10 region single-stranded DNA
23. A promoter melting region in the primary sigma factor of Bacillus subtilis: Identification of functionally important aromatic amino acids
24. Pathway of promoter melting by Bacillus subtilis RNA polymerase at a stable RNA promoter: Effects of temperature, delta protein, and sigma factor mutations
25. The 0°C closed complexes between Escherichia coli RNA polymerase and two promoters, T7-A3 and lacUV5
26. A hybrid sigma subunit directs RNA polymerase to a hybrid promoter in Escherichia coli
27. Characterization of the closed complex intermediate formed during transcription initiation by Escherichia coli RNA polymerase
28. The sigma 70 family: Sequence conservation and evolutionary relationships
29. 70 subunit fragment from E. coli RNA polymerase
30. Promoter recognition as measured by binding of RNA polymerase to nontemplate strand oligonucleotides
31. 70), promoters, and the kinetics of the steps of transcription initiation
32. Kinetics and mechanism of the interaction of E. coli RNA polymerase with the λP(R) promoter
33. Genetic and physiological studies of Bacillus subtilis sigma A mutants defective in promoter melting
34. 4 as a probe for lac promoter DNA melting and mechanism in vivo
35. 70 subunit
36. Inhibition of Escherichia coli RNA polymerase by bacteriophage T4 AsiA
37. Crystal structure of a replication fork single-stranded DNA binding protein (T4 gp32) complexed to DNA
38. 70 subunit of Escherichia coli RNA polymerase
39. Probing the informational content of Escherichia coli sigma 70 region 2.3 by combinatorial cassette mutagenesis
40. Changes in conserved region 2 of Escherichia coli sigma 70 affecting promoter recognition