Enhancing the atom economy of polyketide biosynthetic processes through metabolic engineering

Biotechnology Progress

Volumn 17, Issue 4, 2001, Pages 612-617

  Lombo F ^a   Pfeifer, B ^a   Leaf, T ^b   Ou, S ^b   Kim, Y S ^c   Cane, D E ^d   Licari, P ^b   Khosla, C ^a,e  

^a Stanford University   (United States)

^b KOSAN BIOSCIENCES INC   (United States)

^c Yonsei University   (South Korea)

^d BROWN UNIVERSITY   (United States)

^e STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOACTIVE NATURAL PRODUCTS; METABOLIC ENGINEERING;

BACTERIAL GENE; BACTERIAL PROTEIN; BIOPROCESS; BIOTRANSFORMATION; COENZYME A; FERMENTATION KINETICS; FERMENTATION MEDIUM; HETEROLOGOUS EXPRESSION; HOST; MACROLACTONE 6 DEOXYERYTHRONOLIDE B; METABOLIC ENGINEERING; NATURAL PRODUCT; POLYKETIDE; THIOESTER;

BIOSYNTHESIS; ENZYMES; FERMENTATION; GENES;

BIOTECHNOLOGY;

ACYL COENZYME A; BACTERIAL PROTEINS; COENZYME A LIGASES; DICARBOXYLIC ACID TRANSPORTERS; ERYTHROMYCIN; GENETIC ENGINEERING; METHYLMALONIC ACID; RHIZOBIUM; STREPTOMYCES;

BACTERIA (MICROORGANISMS); RHIZOBIUM LEGUMINOSARUM; STREPTOMYCES COELICOLOR;

EID: 0034877862     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp010045j     Document Type: Article

References (15)

1. The atom economy - A search for synthetic efficiency
2. A gene cluster encoding malonyl-CoA decarboxylase (MatA), malonyl-CoA synthetase (MatB) and a putative dicarboxylate carrier protein (MatC) in Rhizobioum trifolii-Cloning, sequencing, and expression of the enzymes in Escherichia coli
3. Temperature sensitive mutants of Escherichia coli requiring saturated and unsaturated fatty acids for growth: Isolation and properties
4. Identification of residues essential for a. two-step reaction by malonyl-CoA synthetase from Rhizobium trifolii
5. Engineered biosynthesis of a complete macrolactone in a heterologous host
6. Dissecting and exploiting intermodular communication in polyketide synthases
7. Harnessing the biosynthetic code. Combinations, permutations, mutations
8. Tolerance and specificity of polyketide synthases
9. Stereospecific acyl transfers on the erythromycin-preducing polyketide synthase
10. Analysis of the molecular recognition features of individual modules derived from the erythromycin polyketide synthase
11. Overproduction of a functional fatty acid biosynthetic enzyme blocks fatty acid synthesis in Escherichia coli
12. Ratio of active to inactive forms of acyl carrier protein in Escherichia coli
13. Genes encoding acyl-CoA dehydrogenase (AcdH) homologues from Streptomyces coelicolor and Streptomyces avermitilis provide insights into the metabolism of small branched-chain fatty acids and macrolide antibiotic production
14. Genetic and biochemical characterization of the alpha and beta components ora propionyl-CoA carboxylase complex of Streptomyces coelicolor A3(2)