Comparison of anti-p53 antibodies in immunoblotting

Biochemical and Biophysical Research Communications

Volumn 293, Issue 2, 2002, Pages 850-856

  Turpeinen, Miia ^a   Serpi, Raisa ^a   Rahkolin, Mika ^a  

^a UNIVERSITY OF OULU   (Finland)

Author keywords

Anti p53 antibodies; Epitopes; Immunoblotting; MCF 7 cell line; Mouse skin; p53 Homologues

Indexed keywords

BENZO[A]PYRENE; EPITOPE; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; PROTEIN ANTIBODY; PROTEIN P53;

ANIMAL TISSUE; ANTIGEN ANTIBODY REACTION; ARTICLE; BREAST CARCINOMA; CANCER CELL; CONTROLLED STUDY; CROSS REACTION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; MOUSE; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SKIN;

ANIMALS; ANTIBODIES; ANTIBODIES, MONOCLONAL; EPITOPES; FEMALE; HUMANS; IMMUNOBLOTTING; MALE; MICE; MICE, INBRED C57BL; SEQUENCE HOMOLOGY, AMINO ACID; TUMOR CELLS, CULTURED; TUMOR SUPPRESSOR PROTEIN P53;

MINK CELL FOCUS-FORMING VIRUS;

EID: 0036298425     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(02)00298-X     Document Type: Article

References (39)

1. p53 mutations in human cancers
2. The p53 tumour suppressor gene
3. Mutations in the p53 tumour suppressor gene: Clues to cancer etiology and molecular pathogenesis
4. The p53 pathway
5. Activation and activities of the p53 tumour suppressor protein
6. p53: Puzzle and paradigm
7. p53: Emergency brake and target for cancer therapy
8. Linear antigenic sites defined by the B-cell response to human p53 are localized predominantly in the amino and carboxy-termini of the protein
9. Epitope analysis of the murine p53 tumour suppressor protein
10. Regulation of p53 function
11. Detection of p53 in inflammatory tissue and lymphocytes using immunohistology and flow cytometry: A critical comment
12. Specificity of seven monoclonal antibodies against p53 evaluated with western blotting, immunohistochemistry, confocal laser scanning, microscopy and flow cytometry
13. p53 antibodies: Call for quality. Letter to the editor
14. Differences in epitope accessibility of p53 monoclonal antibodies suggest at least three conformations or states of protein binding of p53 protein in human tumor cell lines
15. Analysis of the immunoreactivity of three anti-p53 antibodies and estimation of the relations between p53 status and MDM2 protein expression in ovarian carcinomas
16. Benzo(a)pyrene-7, 8-diol-9, 10-epoxide-DNA adducts and increased p53 protein in mouse skin
17. TPA decreases the p53 response to benzo(a)pyrene-DNA adducts in vivo in mouse skin
18. p53 protein expression is correlated with benzo(a)pyrene-DNA adducts in carcinoma cell lines
19. Thapsigargin has similar effect on p53 protein response to benzo(a)pyrene adducts as TPA in mouse skin
20. Regulation of specific DNA binding by p53: Evidence for a role for O-glycosylation and charged residues at the carboxy-terminus
21. O-glycosylation of eukaryotic transcription factors implications for mechanisms of transcriptional regulation
22. A novel quantative immunoassay system for p53 using antibodies selected for optimum designation of p53 status
23. Genetic and immunochemical analysis of mutant p53 in human breast cancer cell lines
24. Regulation of the specific DNA binding function of p53
25. Regulation of the sequence-specific DNA binding function of p53 by protein kinase C and protein phosphatases
26. Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain
27. Unmasking of phosphorylation-sensitive epitopes on p53 and Mdm2 by a simple Western-phosphatase procedure
28. Monoallelically expressed gene related to p53 at 1p36 a region frequently deleted in neuroblastoma and other human cancers
29. p63, a p53 homolog at 3q27-29, encodes multiple products with transacti-vating, death-inducing and dominant negative activities
30. The p/53/p63/p73 family of transcription factors: Overlapping and distinct functions
31. p53 family update: p73 and p63 develop their own identities
32. The p53 gene family
33. Expression of the p53 homologue p63a and ΔNp63a in normal and neoplastic cells
34. An immuno-chemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53
35. Isolation of human-p53-specific monoclonal antibodies and their use in studies of human p53 expression
36. Selective induction of c-jun and jun-B but not c-fos or c-myc during mitogenesis in SV40-transformed cells at the predi)erentiation growth arrest state
37. Activating mutations in p53 produce a common conformational e)ect. A monoclonal antibody specific for the mutant form
38. Analysis of p53 expression in human tumours: An antibody raised against human p53 expressed Escherichia coli
39. Coupling between gamma irradiation p53 induction and the apoptotic response depends upon cell type in vivo