Glucose-stimulated insulin secretion does not require activation of pyruvate dehydrogenase: Impact of adenovirus-mediated overexpression of PDH kinase and PDH phosphate phosphatase in pancreatic islets

Biochemical and Biophysical Research Communications

Volumn 291, Issue 4, 2002, Pages 1081-1088

  Nicholls, Linda I ^a   Ainscow, Edward K ^a   Rutter, Guy A ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Adenovirus; Glucose; Insulin; Mitochondria; Pyruvate dehydrogenase; cell

Indexed keywords

ADENOSINE TRIPHOSPHATE; DICHLOROACETIC ACID; GLUCOSE; INSULIN; PHOSPHOTRANSFERASE; PYRIDINE NUCLEOTIDE; PYRUVATE DEHYDROGENASE; PYRUVATE DEHYDROGENASE PHOSPHATASE;

ADENOVIRUS; ANIMAL CELL; ARTICLE; CELL CLONE; CONTROLLED STUDY; CYTOSOL; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBUNIT; GENE CONSTRUCT; GLUCOSE METABOLISM; INSULIN RELEASE; MALE; MITOCHONDRION; NONHUMAN; PANCREAS ISLET; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

ADENOVIRIDAE;

EID: 0036297974     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2002.6567     Document Type: Article

References (43)

1. Signal transduction mechanisms in nutrient-induced insulin secretion
2. + channels in mouse B cells
3. Glucose generates subplasma membrane ATP microdomains in single islet beta-cells. Potential role for strategically located mitochondria
4. 2+ to supramicromolar levels in a pancreatic beta-cell line. Possible role in glucose and agonist-induced insulin secretion
5. The hormonal regulation of pyruvate dehydrogenase complex
6. Molecular cloning of the p45 subunit of pyruvate dehydrogenase kinase
7. Regulation of mammalian pyruvate dehydrogenase
8. Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human
9. Evidence for existence of tissue-specific regulation of the mammalian pyruvate dehydrogenase complex
10. Diversity of the pyruvate dehydrogenase kinase gene family in humans
11. Stimulation by calcium ions of pyruvate dehydrogenase phosphate phosphatase
12. Regulation of pyruvate dehydrogenase kinase and phosphatase by acetyl-CoA/CoA and NADH/NAD ratios
13. Cloning, expression, and properties of the regulatory subunit of bovine pyruvate dehydrogenase phosphatase
14. Molecular cloning and expression of the catalytic subunit of bovine pyruvate dehydrogenase phosphatase and sequence similarity with protein phosphatase 2C
15. Purification and properties of pyruvate dehydrogenase phosphatase from bovine heart and kidney
16. Role of the regulatory subunit of bovine pyruvate dehydrogenase phosphatase
17. Glucose-induced activation of pyruvate dehydrogenase in isolated rat pancreatic islets
18. A fatty acid-induced decrease in pyruvate dehydrogenase activity is an important determinant of beta-cell dysfunction in the obese diabetic db/db mouse
19. Deficiency of pyruvate dehydrogenase activity in pancreatic islets of diabetic GK rats
20. Starvation and diabetes increase the amount of pyruvate dehydrogenase kinase isoenzyme 4 in rat heart
21. The pharmacology of dichloroacetate
22. Establishment of a pancreatic beta cell line that retains glucose-inducible insulin secretion: Special reference to expression of glucose transporter isoforms
23. Upstream stimulatory factor-2 (USF2) activity is required for glucose stimulation of L-pyruvate kinase promoter activity in single living islet beta-cells
24. A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues
25. 2+ with recombinant targeted aequorin: Significance for the regulation of pyruvate dehydrogenase activity
26. Acute overexpression of lactate dehydrogenase-A perturbs beta-cell mitochondrial metabolism and insulin secretion
27. A simplified system for generating recombinant adenoviruses
28. Secretory-granule dynamics visualized in vivo with a phogrin-green fluorescent protein chimaera
29. Influence of calcium ions on mammalian intramitochondrial dehydrogenases
30. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
31. Mechanism of activation of pyruvate dehydrogenase by dichloroacetate and other halogenated carboxylic acids
32. Role of NADH shuttle system in glucose-induced activation of mitochondrial metabolism and insulin secretion
33. 2+ oscillations and insulin secretion in pancreatic islets
34. 2+ in aequorin-expressing INS-1 cells
35. Temporal sequence of metabolic and ionic events in glucose-stimulated clonal pancreatic beta-cells (HIT)
36. A control analysis exploration of the role of ATP utilisation in glycolytic-flux control and glycolytic-metabolite-concentration regulation
37. Calcium regulation of oxidative phosphorylation in rat skeletal muscle mitochondria
38. Estimates of glycolysis, pyruvate (de)-carboxylation, pentose phosphate pathway, and methyl succinate metabolism in incapacitated pancreatic islets
39. Glucose-regulated anaplerosis and cataplerosis in pancreatic beta-cells: Possible implication of a pyruvate/citrate shuttle in insulin secretion