Crystallographic and functional studies of a modified form of eosinophil-derived neurotoxin (EDN) with novel biological activities

Journal of Molecular Biology

Volumn 317, Issue 1, 2002, Pages 119-130

  Chang, Changsoo ^a   Newton, Dianne L ^b   Rybak, Susanna M ^a   Wlodawer, Alexander ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b SAIC FREDERICK INC   (United States)

Author keywords

Active site; Atomic resolution; Crystal structure; Cytotoxic ribonuclease; Kaposi sarcoma

Indexed keywords

EOSINOPHIL PROTEIN X; HISTIDINE; RIBONUCLEASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CONTROLLED STUDY; CRYSTALLOGRAPHY; CYTOTOXICITY; DRUG ACTIVITY; ENDOTHELIUM CELL; ENZYME ACTIVITY; HUMAN; HUMAN CELL; KAPOSI SARCOMA; PRIORITY JOURNAL; PROTEIN CONFORMATION; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; TUMOR CELL LINE;

EID: 0036296163     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2002.5406     Document Type: Article

References (42)

1. From housekeeper to microsurgeon: The diagnostic and therapeutic potential of ribonucleases
2. Cytotoxic ribonucleases and chimeras in cancer therapy
3. The return of pancreatic ribonucleases
4. Evolution of antiviral activity in the ribonuclease A gene superfamily: Evidence for a specific interaction between eosinophil-derived neurotoxin (EDN/RNase 2) and respiratory syncytial virus
5. A putative mouse oocyte maturation inhibitory protein from urine of pregnant women: N-terminal sequence homology with human nonsecretory ribonuclease
6. Characterization of a unique nonsecretory ribonuclease from urine of pregnant women
7. Cancer treatment by targeted drug delivery to tumor vasculature in a mouse model
8. Unique recombinant human ribonuclease and inhibition of Kaposi's sarcoma cell growth
9. RNA damage and inhibition of neoplastic endothelial cell growth: Effects of human and amphibian ribonucleases
10. Tumorigenesis and metastasis of neoplastic Kaposi's sarcoma cell line in immunodeficient mice blocked by a human pregnancy hormone
11. X-ray crystallographic structure of recombinant eosinophil-derived neurotoxin at 1.83 Å resolution
12. Mapping the ribonucleolytic active site of eosinophil-derived neurotoxin (EDN). High resolution crystal structures of EDN complexes with adenylic nucleotide inhibitors
13. Stereochemical criteria for polypeptide and protein chain conformations. II Allowed conformation for a pair of peptide units
14. The ultrahigh resolution crystal structure of ribonuclease A containing an isoaspartyl residue: Hydration and sterochemical analysis
15. Multiple conformations of amino acid residues in ribonuclease A
16. Crystal structure of two covalent nucleoside derivatives of ribonuclease A
17. Structure of bovine pancreatic ribonuclease by X-ray and neutron diffraction
18. Crystal structure disposition of thymidylic acid tetramer in complex with ribonuclease A
19. The structures of RNase A complexed with 3′-CMP and d(CpA): Active site conformation and conserved water molecules
20. Crystal structures of ribonuclease A complexes with 5′-diphosphoadenosine 3′-phosphate and 5′-diphosphoadenosine 2′-phosphate at 1.7 Å resolution
21. Toward rational design of ribonuclease inhibitors: High-resolution crystal structure of a ribonuclease A complex with a potent 3′,5′-pyrophosphate-linked dinucleotide inhibitor
22. Ionic interactions in crystalline bovine pancreatic ribonuclease A
23. Eosinophil-derived neurotoxin and human liver ribonuclease. Identity of structure and linkage of neurotoxicity to nuclease activity
24. Isolation and characterization of an immortal neoplastic cell line (KS Y-1) from AIDS-associated Kaposi's sarcoma
25. Comparison of RNases and toxins upon injection into Xenopus oocytes
26. A study of the intracellular routing of cytotoxic ribonucleases
27. Assay for antitumor and lectin activity in RNase homologs
28. Cytotoxic ribonuclease: A study of the mechanism of onconase cytotoxicity
29. Expression and characterization of recombinant human eosinophil-derived neurotoxin and eosinophil-derived neurotoxin-anti-transferrin receptor sFv
30. The biology of E colicins: Paradigms and paradoxes
31. Immuno-enzymes
32. Processing of X-ray diffraction data collected in oscillation mode
33. AMoRe: An automated package for molecular replacement
34. SHELXL: High-resolution refinement
35. Electron-density map interpretation
36. PROCHECK: Program to check the stereochemical quality of protein structures
37. PROMOTIF - A program to identify and analyze structural motifs in proteins
38. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
39. Further additions to MolScript version 1.4, including reading and contouring of electron-density maps
40. Raster3D version 2.0. A program for photorealistic molecular graphics
41. Single amino acid substitutions at the N-terminus of a recombinant cytotoxic ribonuclease markedly influence biochemical and biological properties