Ferric hydroxamate binding protein FhuD from Escherichia coli: Mutants in conserved and non-conserved regions

BioMetals

Volumn 15, Issue 2, 2002, Pages 121-131

  Clarke, Teresa E ^a   Rohrbach, Martin R ^b,c   Tari, Leslie W ^a   Vogel, Hans J ^a   Köster, Wolfgang ^b,d  

^a UNIVERSITY OF CALGARY   (Canada)

^b UNIVERSITY OF TÜBINGEN   (Germany)

^c MediGene AG   (Germany)

^d SWISS FEDERAL INSTITUTE OF AQUATIC SCIENCE AND TECHNOLOGY   (Switzerland)

Author keywords

FhuD; Iron transport; Mutation analysis; Periplasmic protein; Structure analysis

Indexed keywords

ABC TRANSPORTER; BACTERIAL PROTEIN; BINDING PROTEIN; CYANOCOBALAMIN; FERRIC HYDROXAMATE BINDING PROTEIN; HEME DERIVATIVE; IRON COMPLEX; OUTER MEMBRANE PROTEIN; SIDEROPHORE; UNCLASSIFIED DRUG;

ALPHA HELIX; AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL CELL; BACTERIAL MUTATION; CYTOPLASM; ESCHERICHIA COLI; IRON METABOLISM; IRON TRANSPORT; LIGAND BINDING; PEPTIDE MAPPING; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; CARRIER PROTEINS; CONSERVED SEQUENCE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; EVOLUTION, MOLECULAR; FERRIC COMPOUNDS; HYDROXAMIC ACIDS; MEMBRANE TRANSPORT PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PERIPLASMIC BINDING PROTEINS; PHENOTYPE; PHYLOGENY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA;

EID: 0036260950     PISSN: 09660844     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1015249530156     Document Type: Article

References (35)

1. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
2. Bacterial periplasmic transport systems: Structure, function and evolution
3. Conserved amino acids in the N- and C-terminal domains of integral membrane transporter FhuB define sites important for intra- and intermolecular interaction
4. Pumping iron through cell membranes
5. Bacterial iron transport, mechanisms, genetics, and regulation
6. Bacterial solutions to the iron-supply problem
7. Crystal structure of the outer membrane active transporter FepA from Escherichia coli
8. Microbial iron transport: Iron acquisition by pathogenic microorganisms
9. The structure of the ferric siderophore binding protein FhuD complexed with gallichrome
10. Structural biology of iron uptake systems
11. Effect of pyochelin on the virulence of Pseudomonas aeruginosa
12. SETOR: Hardware-lighted three-dimensional solid model representations of macromolecules
13. Crystal structure of the antibiotic albomycin in complex with the outer membrane transporter FhuA
14. Siderophore-mediated iron transport: Crystal structure of FhuA with bound lipopolysaccharide
15. 12
16. Iron (III) hydroxamate transport across the cytoplasmic membrane of Escherichia coli
17. 12
18. Iron (III) hydroxamate transport into Escherichia coli. Substrate binding to the periplasmic FhuD protein
19. The Escherichia coli ATP-binding cassette (ABC) proteins
20. Transmembrane signaling across the ligand-gated FhuA receptor: Crystal structures of free and ferrichrome-bound states reveal allosteric changes
21. ATP-dependent ferric hydroxamate transport system in Escherichia coli: Periplasmic FhuD interacts with a periplasmic and with a transmembrane/cytoplasmic region of the integral membrane protein FhuB, as revealed by competitive peptide mapping
22. Hinge-bending in L-arabinose-binding protein. The 'Venus-flytrap' model
23. Iron and virulence in the family Enterobacteriaceae
24. Iron acquisition in microbial pathogenesis
25. Atomic structures of periplasmic binding proteins and the high-affinity active transport systems in bacteria
26. Atomic structure and specificity of bacterial periplasmic receptors for active transport and chemotaxis: Variation of common themes
27. Iron metabolism in pathogenic bacteria
28. Ferrichrome transport in Escherichia coli K-12: Altered substrate specificity of mutated periplasmic FhuD and interaction of FhuD with the integral membrane protein FhuB
29. In vivo reconstitution of an active siderophore transport system by a binding protein derivative lacking a signal sequence
30. Getting in or out: Early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters
31. Iron acquisition systems in the pathogenic Neisseria
32. Structural, functional, and evolutionary relationships among extracellular solute-binding receptors of bacteria
33. The response of Pseudomonas aeruginosa to iron: Genetics, biochemistry and virulence
34. Bacterial heme sources: The role of heme, hemoprotein receptors and hemophores