Conserved amino acids in the N- and C-terminal domains of integral membrane transporter FhuB define sites important for intra- and intermolecular interactions

Molecular Microbiology

Volumn 20, Issue 1, 1996, Pages 223-232

  Böhm, Brigitte ^a   Beschert, Hartmut ^a   Köster, Wolfgang ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROXAMIC ACID DERIVATIVE; HYDROXAMIC ACID IRON DERIVATIVE; MEMBRANE PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; MEMBRANE TRANSPORT; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CONSERVED SEQUENCE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FERRIC COMPOUNDS; GENETIC COMPLEMENTATION TEST; HYDROXAMIC ACIDS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; PERIPLASMIC BINDING PROTEINS; POINT MUTATION;

EID: 0029868045     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2958.1996.tb02503.x     Document Type: Article

References (51)

1. Bibi, E., and Kaback, H.R. (1992) Functional complementation of internal deletion mutants in the lactose permease of Escherichia coli. Proc Natl Acad Sci USA 89: 1524-1528.
2. Boyd, D., Traxler, B., and Beckwith, J. (1993) Analysis of the topology of a membrane protein by using a minimum number of alkaline phosphatase fusions. J Bacteriol 175: 553-556.
3. Braun, V., and Hantke, K. (1991) Genetics of bacterial iron transport. In Handbook of Microbial Iron Chelates. Winkelmann, G. (ed.). Boca Raton: CRC Press, pp. 107-138.
4. Braun, V., Gross, R., Köster, W., and Zimmermann, L. (1983) Plasmid and chromosomal mutants in the iron(III)-aerobactin transport system of Escherichia coli. Use of streptonigrin for selection. Mol Gen Genet 192: 131-139.
5. Braun, V., Hantke, K., Eick-Helmerich, K., Köster, W., Preßler, U., Sauer, M., Schärfer, S., Schöffler, H., Staudenmaier, H., and Zimmermann, L. (1987) Iron transport systems in Escherichia coli. In Iron Transport in Microbes, Plants and Animals. Winkelmann, G., van der Helm, D., and Neilands, J.B. VCH Verlagsgesellschaft, Weinheim, pp. 35-51.
6. Burkhardt, R., and Braun, V. (1987) Nucleotide sequence of fhuC and fhuD genes involved in iron (III)-hydroxamate transport: domains in FhuC homologous to ATP-binding proteins. Mol Gen Genet 209: 49-55.
7. Chenault, S.S., and Earhart, C.F. (1991) Organization of genes encoding membrane proteins of the Escherichia coli ferrienterobactin permease. Mol Microbiol 5: 1405-1413.
8. Coulton, J.W., Mason, P., and Allat, D.D. (1987) fhuC and fhuD genes for iron(III)-ferrichrome transport into Escherichia coli K-12. J Bacteriol 169: 3844-3849.
9. Dassa, E. (1990) Cellular localization of the MalG protein from the maltose transport system in Escherichia coli. Mol Gen Genet 222: 32-36.
10. Dassa, E., and Hofnung, M. (1985) Sequence of malG gene in E. coli K-12: homologies between integral membrane components from binding protein-dependent transport systems. EMBO J 4: 2287-2293.
11. Dassa, E., and Muir, S. (1993) Membrane topology of MalG, an inner membrane protein from the maltose transport system of Escherichia coli. Mol Microbiol 7: 29-38.
12. Davidson, A.L., and Nikaido, H. (1990) Overproduction, solubilization, and reconstitution of the maltose transport system from Escherichia coli. J Biol Chem 265:4254-4260.
13. Davidson, A.L., and Nikaido, H. (1991) Purification and characterization of the membrane-associated components of the maltose transport system from Escherichia coli. J Biol Chem 266: 8946-8951.
14. Davidson, A.L., Shuman, H.A., and Nikaido, H. (1992) Mechanism of maltose transport in Escherichia coli: transmembrane signaling by periplasmic binding proteins. Proc Natl Acad Sci USA 89: 2360-2364.
15. Eichenlaub, R. (1984) Isolation of suppressible mutations by hydroxylamine mutagenesis in vitro. In Advanced Molecular Genetics. Pühler, A., and Timmis, K.N. (eds). Berlin: Springer Verlag, pp. 106-110.
16. Fecker, L., and Braun, V. (1983) Cloning and expression of the fhu genes involved in iron (III) hydroxamate uptake by E. coli. J Bacteriol 156: 1301-1314.
17. Fiss, E.H., Shengwei, Y., and Jacobs, Jr, W.R. (1994) Identification of genes involved in the sequestration of iron in mycobacteria: the ferric exochelin biosynthetic and uptake pathways. Mol Microbiol 14: 557-569.
18. Fleischmann, R.D. et al. (1995) Whole-genome random sequencing and assembly of Haemophilus influenzae. Science 269: 496-512.
19. Friedrich, M.J., DeVeaux, L.C., and Kadner, R.J. (1986) Nucleotide sequence of the btuCED genes involved in vitamin B12 transport in Escherichia coli and homology with components of periplasmic binding protein-dependent transport systems. J Bacteriol 167: 928-934.
20. Froshauer, S., Green, G.N., Boyd, D., McGovern, K., and Beckwith, J. (1988) Genetic analysis of the membrane insertion and topology of MalF, a cytoplasmic membrane protein of E. coli. J Mol Biol 200: 501-511.
21. Hershkovitz, I. (1987) Functional inactivation of genes by dominant negative mutations. Nature 329: 219-222.
22. 12 transport in Escherichia coli: energy coupling between membranes. Mol Microbiol 4: 2027-2033.
23. 12 in Escherichia coli. In Iron Transport in Microbes, Plants and Animals. Winkelmann, G., van der Helm, D., and Neilands, J.B. VCH Verlagsgesellschaft, pp. 85-97.
24. Kerppola, R.E., and Ames, G.F.L. (1992) Topology of the hydrophobic membrane-bound components of the histidine periplasmic permease. Comparisons with other members of the family. J Biol Chem 267: 2329-2336.
25. Kerppola, R.E., Shyamala, V.K., Klebba, P., and Ames, G.F.-L. (1991) The membrane-bound proteins of periplasmic permeases form a complex - identification of the histidine permease HisQMP complex. J Biol Chem 266: 9857-9865.
26. Köster, W. (1991) Iron (III) hydroxamate transport across the cytoplasmic membrane of Escherichia coli. Biol Metals 4: 23-32.
27. Köster, W., and Böhm, B. (1992) Point mutations in 2 conserved glycine residues within the integral membrane protein FhuB affect iron (III) hydroxamate transport. Mol Gen Genet 232: 399-407.
28. Köster, W., and Braun, V. (1986) Iron hydroxamate transport of Escherichia coli: nucleotide sequence of the fhuB gene and identification of the protein. Mol Gen Genet 204: 435-442.
29. Köster, W., and Braun, V. (1989) Iron hydroxamate transport into Escherichia coli K-12: localization of FhuD in the periplasm and of FhuB in the cytoplasmic membrane. Mol Gen Genet 217: 435-442.
30. Köster, W., and Braun, V. (1990a) Iron (III) hydroxamate transport into Escherichia coli. Substrate binding to the periplasmic binding protein. J Biol Chem 266: 21407-21410.
31. Köster, W., and Braun, V. (1990b) Iron (III) hydroxamate transport of Escherichia coli: restoration of iron supply by co-expression of the N- and C-terminal halves of the cytoplasmic membrane protein FhuB cloned on separate plasmids. Mol Gen Genet 223: 379-384.
32. Köster, W.L., Actis, L.A., Waldbeser, L.S., Tolmasky, M.E., and Crosa, J.H. (1991) Molecular characterization of the iron transport system mediated by the pJM1-plasmid in Vibrio anguillarum 775. J Biol Chem 266: 23829-23833.
33. Lugtenberg, B., Meijers, J., Peters, E., van der Hoeck, P., and van Alphen, L. (1975) Electrophoretic resolution of the major outer membrane protein of Escherichia coli K-12 into four bands. FEBS Lett 58: 254-258.
34. McGovern, K., Ehrmann, M., and Beckwith, J. (1991). Decoding signals for membrane protein assembly using alkaline phosphatase fusions. EMBO J 10: 2773-2782.
35. Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
36. Martinez, E., Bartolome, B., and de la Cruz, F. (1988) pACYC184-derived cloning vectors containing the multiple cloning site and lacZ reporter gene of pUC8/9 and pUC18/ 19 plasmids. Gene 68: 159-162.
37. Mimmack, M.L, Gallagher, M.P., Pearce, S.R., Hyde, S.C., Booth, I.R., and Higgins, C.F. (1989) Energy coupling to perrplasmic binding protein-dependent transport systems: stoichiometry of ATP hydrolysis during transport in vivo. Proc Natl Acad Sci USA 86: 8257-8260.
38. Pearce, S.R., Mimmack, M.L., Gallagher, M.P., Gileadi, U., Hyde, S.C., and Higgins, C.F. (1992) Membrane topology of the integral membrane components, OppB and OppC, of the oligopeptide permease of Salmonella typhimurium. Mol Microbiol 6: 47-57.
39. Popot, J.L. (1993) Integral membrane structure: transmembrane α-helices as autonomous folding domains. Curr Op Struct Biol 3: 532-540.
40. Popot, J.L., De Vitry, C., and Atteia, A. (1994) Folding and assembly of integral membrane proteins: an introduction. In Membrane Protein Structure. White, S.H. (ed). Oxford: Oxford University Press, pp. 41-96.
41. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
42. Saurin, W., Köster, W., and Dassa, E. (1994) Bacterial binding protein-dependent permeases: characterization of distinctive signatures for functionally related integral membrane proteins. Mol Microbiol 12: 993-1004.
43. Schultz-Hauser, G., Köster, W., Schwarz, H., and Braun, V. (1992a) Iron (III) hydroxamate transport in Escherichia coli K-12: FhuB-mediated membrane association of the FhuC protein and negative complementation of fhuC mutants. J Bacteriol 174: 2305-2311.
44. Schultz-Hauser, G., Van Hove, B., and Braun, V. (1992b) 8-Azido-ATP labelling of the FecE protein of the Escherichia coli iron citrate transport system. FEMS Microbiol Lett 95: 231-234.
45. Shea, C.M., and McIntosh, M.A. (1991) Nucleotide sequence and genetic organization of the ferric enterobactin transport system - homology to other periplasmic binding protein-dependent systems in Escherichia coli. Mol Microbiol 5: 1415-1428.
46. Short, J.M., Fernandez, J.A., Sorge, J.A., and Huse, W.D. (1988) Lambda zap: a bacteriophage lambda expression vector with in vivo excision properties. Nuc Acid Res 16: 7583-7600.
47. Staudenmaier, H., Van Hove, B., Yaraghi, Z., and Braun, V. (1989) Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic binding protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli. J Bacteriol 171: 626-2633.
48. Stojiljkovic, I., and Hantke, K. (1994) Transport of haemin across the cytoplasmic membrane through a haemin-specific periplasmic binding protein-dependent transport system in Yersinia enterocolitica. Mol Microbiol 13: 719-732.
49. Tabor, S., and Richardson, C.C. (1985) A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci USA 82: 1074-1078.
50. Traxler, B., and Beckwith, J. (1992) Assembly of a heterooligomeric membrane protein complex. Proc Natl Acad Sci USA 89: 10852-10856.
51. Whitley, P., Zander, T., Ehrmann, M., Haardt, M., Bremer, E., and von Heijne, G. (1994) Sec-independent translocation of a 100-residue periplasmic N-terminal tail in the E. coli inner membrane protein ProW. EMBO J 13: 4653-4661.