Crosslinking of 4.5S RNA to the Escherichia coli ribosome in the presence or absence of the protein Ffh

RNA

Volumn 8, Issue 5, 2002, Pages 612-625

  Rinke Appel, Jutta ^a   Osswald, Monika ^a   Von Knoblauch, Klaus ^a   Mueller, Florian ^a   Brimacombe, Richard ^a   Sergiev, Petr ^a   Avdeeva, Olga ^a   Bogdanov, Alexey ^a   Dontsova, Olga ^a  

^a MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

Author keywords

Elongation factor G; Functions of 4.5S RNA; Nascent peptide chain; Ribosome structure; Signal recognition particle

Indexed keywords

ELONGATION FACTOR G; PROTEIN DERIVATIVE; PROTEIN FFH; RIBOSOME RNA; RNA 23S; SIGNAL RECOGNITION PARTICLE; THIOURIDINE; TRANSFER RNA; UNCLASSIFIED DRUG;

ARTICLE; AUTORADIOGRAPHY; BINDING SITE; COMPLEX FORMATION; COMPUTER MODEL; CONTROLLED STUDY; CROSS LINKING; CRYSTAL STRUCTURE; ESCHERICHIA COLI; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RIBOSOME;

BASE SEQUENCE; BINDING SITES; CROSS-LINKING REAGENTS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MACROMOLECULAR SUBSTANCES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PROTEIN CONFORMATION; RIBONUCLEASE H, CALF THYMUS; RIBOSOMES; RNA, BACTERIAL; RNA, RIBOSOMAL; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 23S; SIGNAL RECOGNITION PARTICLE; THIOURIDINE;

ESCHERICHIA COLI;

EID: 0036256166     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838202020095     Document Type: Article

References (42)

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3. The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution
4. Crystal structure of the ribonucleoprotein core of the signal recognition particle
5. New techniques for the analysis of intra-RNA and RNA-protein cross-linking data from ribosomes
6. Three-dimensional organization of the bacterial ribosome and its subunits: The transition from low-resolution models to high-resolution structures
7. Time of action of 4.5S RNA in E. coli translation
8. The path of the growing peptide chain through the 23S rRNA in the 50S ribosomal subunit; a comparative cross-linking study with three different peptide families
9. Loop IV of 5S ribosomal RNA has contacts both to domain II and to domain V of the 23S RNA
10. Three widely separated positions in the 16S RNA lie in or close to the ribosomal decoding region; a site-directed cross-linking study with mRNA analogues
11. Stemloop IV of 5S ribosomal RNA lies close to the peptidyl transferase center
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13. RNA-protein cross-linking in E. coli 50S ribosomal subunits; determination of sites on 23S RNA that are cross-linked to proteins L2, L4, L24, and L27 by treatment with 2-iminothiolane
14. Requirements for the membrane insertion of signal-anchor type proteins
15. Concentrations of 4.5S RNA and Ffh protein in E. coli: The stability of Ffh protein is dependent on the concentration of 4,5S RNA
16. Crystal structure of the Ffh and EF-G binding sites in the conserved domain IV of E. coli 4.5S RNA
17. Crystal structure of the signal sequence binding subunit of the signal recognition particle
18. Formation of SRP-like particle induces a conformation change in E. coli 4.5S RNA
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23. Minimal functional structure of E. coli 4.5S RNA required for binding to elongation factor G
24. Genetic probes of ribosomal RNA function
25. An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle
26. Parameters for the preparation of E. coli ribosomes and ribosomal subunits active in tRNA binding
27. E coli 4.5S RNA is part of a ribonucleoprotein particle that has properties related to signal recognition particle
28. The path of mRNA through the ribosome; site-directed cross-linking of mRNA analogues carrying a photo-reactive label at various points 3′ to the decoding site
29. Site-directed cross-linking of mRNA analogues to 16S ribosomal RNA; a complete scan of cross-links from all positions between "+1" and "+16" on the mRNA, down-stream from the decoding site
30. The 54-kD protein of signal recognition particle contains a methionine-rich RNA binding domain
31. Structure of functionally activated small ribosomal subunit at 3.3 Å resolution
32. NMR studies of the most conserved RNA domain of the mammalian signal recognition particle
33. Visualization of protein S1 within the 30S ribosomal subunit and its interaction with messenger RNA
34. Correlating the X-ray structures for halo- and thermophilic ribosomal subunits with biochemical data for the E. coli ribosome
35. Identification of protein synthesis elongation factor G as a 4.5S RNA-binding protein in E. coli
36. Mapping the path of the nascent peptide chain through the 23S RNA in the 50S ribosomal subunit
37. Site-directed cross-linking of mRNA analogues to the E. coli ribosome; identification of 30S ribosomal components that can be cross-linked to the mRNA at various points 5′ with respect to the decoding site
38. Visualization of elongation factor Tu on the E. coli ribosome
39. Large-scale movement of elongation factor G and extensive conformational change of the ribosome during translocation
40. Analysis of E coli 4.5S RNA binding affinity to Ffh and EF-G
41. Structure of the 30S ribosomal subunit
42. The methioninerich domain of the 54 kd protein subunit of the signal recognition particle contains an RNA binding site and can be cross-linked to a signal sequence