Crystal structure of the ribonucleoprotein core of the signal recognition particle

Science

Volumn 287, Issue 5456, 2000, Pages 1232-1239

  Batey, Robert T ^a   Rambo, Robert P ^a   Lucast, Louise ^a   Rha, Brian ^a   Doudna, Jennifer A ^a  

^a YALE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA; SIGNAL RECOGNITION PARTICLE;

ARTICLE; BINDING SITE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN RNA BINDING; RNA BINDING;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE PAIRING; BINDING SITES; CELL MEMBRANE; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GUANOSINE TRIPHOSPHATE; HYDROGEN BONDING; MAGNESIUM; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; POTASSIUM; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RNA, BACTERIAL; SIGNAL RECOGNITION PARTICLE; TRANSFORMATION, BACTERIAL; WATER;

EID: 0034681490     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.287.5456.1232     Document Type: Article

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52. We thank S. Brown for providing the 4.55 RNA conditionally deficient strains of E. coli and helpful advice; C. Ogata for time and assistance at beamline X4A of the National Synchrotron Light Source, Brookhaven National Laboratory; T. Earnest for support at beamline 5.0.2 of the Advanced Light Source, Lawrence Berkeley National Laboratory; R. Hanna, J. Kieft, A. Luptak, and M. Talavera for help with beam-line data collection and useful discussions; P. Adams and L. Rice for assistance with the refinement; the staff of the Yale Center for Structural Biology for cristallographic and computational support; S. Strobel for providing the nucleotide analogs and for helpful discussions; S. Basu, J. Cate, J. Davis, T. Griffin, J. Ippolito, S. Ryder, P. Scamborova, P. Sigler, and T. Steitz for many helpful discussions; and J. Cate, J. Kieft, V. Rath, and S. Strobel for critically reading the manuscript. We are particularly indebted to A. Ferré-D'Amaré for excellent advice and support throughout this project. This project was funded in part by a postdoctoral fellowship from the Jane Coffin Childs Memorial Fund for Medical Research (R.T.B.), a Howard Hughes Medical Institute (HHMI) summer fellowship (B.R.), and by support from the NIH, HHMI, and the David and Lucile Packard Foundation. J.A.D. is a Fellow of the David and Lucile Packard Foundation and an Assistant Investigator of HHMI. Coordinates of the SRP core and structure factor amplitudes have been deposited in the PDB (accession code 1DUL). Coordinates are also available at www.csb.yale.edu/ people/doudna/doudna_people.html.