Functional analysis of the signal recognition particle in Escherichia coli by characterization of a temperature-sensitive ffh mutant

Journal of Bacteriology

Volumn 184, Issue 10, 2002, Pages 2642-2653

  Park, Sei Kyoung ^a   Jiang, Fenglei ^a   Dalbey, Ross E ^a   Phillips, Gregory J ^a  

^a IOWA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; HEAT SHOCK PROTEIN; MEMBRANE PROTEIN; PROTEIN FFH; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BACTERIUM MUTANT; CELL VIABILITY; CONTROLLED STUDY; ESCHERICHIA COLI; GENE FUNCTION; GENE INSERTION; GENE ISOLATION; GENETIC PROCEDURES; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN STABILITY; SEQUENCE HOMOLOGY; TEMPERATURE DEPENDENCE; TEMPERATURE SENSITIVITY;

ALLELES; BACTERIAL PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HEAT-SHOCK PROTEINS; MEMBRANE PROTEINS; MUTATION; RNA, RIBOSOMAL; SIGNAL RECOGNITION PARTICLE; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; INSERTION SEQUENCES; NEGIBACTERIA;

EID: 0036241637     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.10.2642-2653.2002     Document Type: Article

References (73)

1. A gene regulating the heat shock response in Escherichia coli also affects proteolysis
2. The primary pathway of protein export in E. coli
3. Crystal structure of the ribonucleoprotein core of the signal recognition particle
4. Sequence gazing?
5. Requirements of the translocation of elongationarrested, ribosome-associated OmpA across the plasma membrane of Escherichia coli
6. Physiological basis for conservation of the signal recognition particle targeting pathway in Escherichia coli
7. Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle
8. Functional substitution of the signal recognition particle 54-kDa subunit by its Escherichia coli homolog
9. Loss of 4.5S RNA induces the heat shock response and lambda prophage in Escherichia coli
10. Time of action of 4.5 S RNA in Escherichia coli translation
11. The 4.5 S RNA gene of Escherichia coli is essential for cell growth
12. The nucleotide sequence of an Escherichia coli operon containing genes for the tRNA(m1G)methyltransferase the ribosomal proteins S16 and L19 and a 21-K polypeptide
13. An inexpensive and simple method for DNA purifications on silica particles
14. Leader peptidase catalyzes the release of exported proteins from the outer surface of the Escherichia coli plasma membrane
15. Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle
16. Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli
17. Green fluorescent protein functions as a reporter for protein localization in Escherichia coli
18. A new cell division operon in Escherichia coli
19. SRPDB (signal recognition particle database)
20. Cloning and sequence analysis of the Escherichia coli 4.5 S RNA gene
21. High efficiency transformation of Escherichia coli with plasmids
22. Diverse effects of the MalE-LacZ hybrid protein on Escherichia coli cell physiology
23. Biotinylation in vivo as a sensitive indicator of protein secretion and membrane protein insertion
24. Effect of 4.5S RNA depletion on Escherichia coli protein synthesis and secretion
25. Concentrations of 4.5S RNA and Ffh protein in Escherichia coli: The stability of Ffh protein is dependent on the concentration of 4.5S RNA
26. The signal recognition particle
27. Crystal structure of the signal sequence binding subunit of the signal recognition particle
28. Is Ffh required for export of secretory proteins?
29. In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli
30. Engineered gene for Escherichia coli alkaline phosphatase for the construction of translational fusions
31. Mutations in a new gene, secB, cause defective protein localization in Escherichia coli
32. Effects of mutations in heat-shock genes groES and groEL on protein export in Escherichia coli
33. The Escherichia coli heat shock proteins GroEL and GroES modulate the folding of the β-lactamase precursor
34. The targeting pathway of Escherichia coli presecretory and integral membrane proteins is specified by the hydrophobicity of the targeting signal
35. Pleiotropic changes resulting from depletion of Era, an essential GTP-binding protein in Escherichia coli
36. Random mutagenesis of Schizosaccharomyces pombe SRP RNA: Lethal and conditional lesions cluster in presumptive protein binding sites
37. Signal-sequence recognition by an Escherichia coli ribonucleoprotein complex
38. An alternative protein targeting pathway in Escherichia coli: Studies on the role of FtsY
39. Genes acrA and acrB encode a stress-induced efflux system of Escherichia coli
40. The functional integration of a polytopic membrane protein of Escherichia coli is dependent on the bacterial signal-recognition particle
41. Minimal functional structure of Escherichia coli 4.5 S RNA required for binding to elongation factor G
42. The structure of multiple polypeptide domains determines the signal recognition particle targeting requirement of Escherichia coli inner membrane proteins
43. E. coli mutant pleiotropically defective in the export of secreted proteins
44. High copy number plasmids compatible with commonly used cloning vectors
45. The E. coli ffh gene is necessary for viability and efficient protein export
46. Genetic system for reversible integration of DNA constructs and lacZ gene fusions into the Escherichia coli chromosome
47. An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle
48. Human SRP RNA and E. coli 4.5S RNA contain a highly homologous structural domain
49. E. coli 4.5S RNA is part of a ribonucleoprotein particle that has properties related to signal recognition particle
50. Homology of 54K protein of signal-recognition particle, docking protein and two E. coli proteins with putative GTP-binding domains
51. YidC mediates membrane protein insertion in bacteria
52. The secE gene encodes an integral membrane protein required for protein export in Escherichia coli
53. Direct evidence that the proton motive force inhibits membrane translocation of positively charged residues within membrane proteins
54. FtsY, the prokaryotic signal recognition particle receptor homologue, is essential for biogenesis of membrane proteins
55. A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: Isolation and characterization of a new temperature-sensitive secY mutation
56. Analysis of Escherichia coli 4.5S RNA binding affinity to Ffh and EF-G
57. Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing heat-shock proteins
58. Genetic screen yields mutations in genes encoding all known components of the Escherichia coli signal recognition particle pathway
59. A mutant hunt for defects in membrane protein assembly yields mutations affecting the bacterial signal recognition particle and Sec machinery
60. The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins
61. Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor
62. The Escherichia coli SRP and SecB targeting pathways converge at the translocon
63. Acetylornithinase of Escherichia coli: Partial purification and some properties
64. Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum
65. Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum
66. Involvement of the DnaK-DnaJ-GrpE chaperone team in protein secretion in Escherichia coli
67. Accumulation of secretory protein precursors in Escherichia coli induces the heat shock response
68. Evolutionary conserved nucleotides within the E. coli 4.5S RNA are required for association with P48 in vitro and for optimal function in vivo
69. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the Ml3mpl8 and pUC19 vectors
70. Domain interactions in E. coli SRP: Stabilization of M domain by RNA is required for effective signal sequence modulation of NG domain