Genetic screen yields mutations in genes encoding all known components of the Escherichia coli signal recognition particle pathway

Journal of Bacteriology

Volumn 184, Issue 1, 2002, Pages 111-118

  Tian, H ^a   Beckwith, J ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MEMBRANE PROTEIN; PROTEIN ACRB; PROTEIN FFH; PROTEIN FFS; PROTEIN FTSQ; PROTEIN FTSY; PROTEIN MA1F; RNA; SIGNAL RECOGNITION PARTICLE; UNCLASSIFIED DRUG;

ARTICLE; BIOTINYLATION; CELL MEMBRANE; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; GENE INSERTION; GENE MUTATION; GENE TRANSLOCATION; GENETIC SCREENING; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; SIGNAL TRANSDUCTION;

ADENOSINE TRIPHOSPHATASES; ATP-BINDING CASSETTE TRANSPORTERS; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; CARRIER PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, BACTERIAL; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MONOSACCHARIDE TRANSPORT PROTEINS; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; MUTATION; PERIPLASM; PROTEIN TRANSPORT; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINANT FUSION PROTEINS; RNA, RIBOSOMAL; SELECTION (GENETICS); SIGNAL RECOGNITION PARTICLE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; INSERTION SEQUENCES; NEGIBACTERIA;

EID: 0036135526     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.1.111-118.2002     Document Type: Article

References (48)

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2. Crystal structure of the ribonucleoprotein core of the signal recognition particle
3. Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle
4. OXA1, a Saccharomyces cerevisiae nuclear gene whose sequence is conserved from prokaryotes to eukaryotes controls cytochrome oxidase biogenesis
5. The 4.5 S RNA gene of Escherichia coli is essential for cell growth
6. The signal recognition particle receptor mediates the GTP-dependent displacement of SRP from the signal sequence of the nascent polypeptide
7. Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle
8. Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide
9. The signal sequence interacts with the methionine-rich domain of the 54-kD protein of signal recognition particle
10. Biotinylation in vivo as a sensitive indicator of protein secretion and membrane protein insertion
11. Regulation of the Escherichia coli secA gene is mediated by two distinct RNA structural conformations
12. In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli
13. Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle
14. An alternative protein targeting pathway in Escherichia coli: Studies on the role of FtsY
15. Functional integration of a polytopic membrane protein of E. coli requires the bacterial signal recognition particle
16. Membrane insertion of the Escherichia coli Ma1F protein in cells with impaired secretion machinery
17. Secretion monitor SecM, undergoes self-translation arrest in the cytosol
18. The N-domain of the signal recognition particle 54-kDa subunit promotes efficient signal sequence binding
19. Regulation of a membrane component required for protein secretion in Escherichia coli
20. The E. coli ffh gene is necessary for viability and efficient protein export
21. An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle
22. Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor
23. The first gene in the Escherichia coli secA operon, gene X, encodes a nonessential secretory protein
24. Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes
25. Escherichia coli exports previously folded and biotinated protein domains
26. A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene
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28. YidC mediates membrane protein insertion in bacteria
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30. Regulation of Escherichia coli secA mRNA translation by a secretion-responsive element
31. SecA protein autogenously represses its own translation during normal protein secretion in Escherichia coli
32. Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli
33. FtsY, the prokaryotic signal recognition particle receptor homologue, is essential for biogenesis of membrane proteins
34. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli
35. Common structural features between eukaryotic 7SL RNAs, eubacterial 4.5S RNA and scRNA and archaebacterial 7S RNA
36. The signal recognition particle receptor is a complex that contains two distinct polypeptide chains
37. A mutant hunt for defects in membrane protein assembly yields mutations affecting the bacterial signal recognition particle and Sec machinery
38. Insertion of the polytopic membrane protein Ma1F is dependent on the bacterial secretion machinery
39. The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins
40. Early events in preprotein recognition in E. coli: Interaction of SRP and trigger factor with nascent polypeptides
41. The Escherichia coli SRP and SecB targeting pathways converge at the translocon
42. Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues
43. Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum
44. Mechanism of protein translocation across the endoplasmic reticulum membrane
45. Membrane insertion of the mannitol permease of Escherichia coli occurs under conditions of impaired SecA function
46. Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli
47. Evolutionary conserved nucleotides within the E. coli 4.5S RNA are required for association with P48 in vitro and for optimal function in vivo
48. The methionine-rich domain of the 54 kd protein subunit of the signal recognition particle contains an RNA binding site and can be crosslinked to a signal sequence