A hammerhead ribozyme substrate and reporter for in vitro kinetoplastid RNA editing

RNA

Volumn 8, Issue 4, 2002, Pages 548-554

  Wang, Bingbing ^a   Salavati, Reza ^a   Heidmann, Stefan ^a   Stuart, Kenneth ^a  

^a SEATTLE BIOMEDICAL RESEARCH INSTITUTE   (United States)

Author keywords

GRNA; Trypanosoma brucei; Uridylate deletion; Uridylate insertion

Indexed keywords

HAMMERHEAD RIBOZYME; MESSENGER RNA PRECURSOR; RIBOZYME; UNCLASSIFIED DRUG; URIDINE PHOSPHATE;

ARTICLE; DIAGNOSTIC TEST; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; KINETOPLAST; MITOCHONDRION; PRIORITY JOURNAL; RNA EDITING; RNA PROCESSING; RNA SEQUENCE; SENSITIVITY AND SPECIFICITY; TRYPANOSOMA BRUCEI;

ANIMALS; BASE SEQUENCE; MOLECULAR SEQUENCE DATA; RNA EDITING; RNA, CATALYTIC; TRYPANOSOMA BRUCEI BRUCEI; URIDINE MONOPHOSPHATE;

INSERTION SEQUENCES; KINETOPLASTIDA; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0036236872     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S135583820202962X     Document Type: Article

References (23)

1. The mechanism of U insertion/deletion RNA editing in kinetoplastid mitochondria
2. Guide RNAs in kinetoplastid mitochondria have a nonencoded 3′ oligo(U) tail involved in recognition of the preedited region
3. The effect of structure in a long target RNA on ribozyme cleavage efficiency
4. Hammerhead ribozymes with a faster cleavage rate
5. Trypanosome RNA editing: Simple guide RNA features enhance U deletion 100-fold
6. Substrate sequence effects on "hammerhead" RNA catalytic efficiency
7. Simple RNA enzymes with new and highly specific endoribonuclease activities
8. Unexpected anisotropy in substrate cleavage rates by asymmetric hammerhead ribozymes
9. Specificity of hammerhead ribozyme cleavage
10. RNA editing: A mechanism for gRNA-specified uridylate insertion into precursor mRNA
11. Sequence specificity of the hammerhead ribozyme revisited; the NHH rule
12. Mapping contacts between gRNA and mRNA in trypanosome RNA editing
13. Association of two novel proteins, TbMP52 and TbMP48, with the Trypanosoma brucei RNA editing complex
14. Organization of minicircle genes for guide RNAs in Trypanosoma brucei
15. Assembly of mitochondrial ribonucleoprotein complexes involves specific guide RNA (gRNA)-binding proteins and gRNA domains but does not require preedited mRNA
16. Sequence requirements of the hammerhead RNA self-cleavage reaction
17. Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria
18. Direct visualization of uridylate deletion in vitro suggests a mechanism for kinetoplastid RNA editing
19. RNA editing: Transfer of genetic information from gRNA to precursor mRNA in vitro
20. RNA editing in kinetoplastid protozoa
21. Small catalytic RNAs
22. A small catalytic oligoribonucleotide
23. Function of specific 2′-hydroxyl groups of guanosines in a hammerhead ribozyme probed by 2′ modifications