Sequence-specific gene cleavage in intact mammalian cells by 125I-labeled triplex-forming oligonucleotides conjugated with nuclear localization signal peptide

Antisense and Nucleic Acid Drug Development

Volumn 12, Issue 1, 2002, Pages 43-49

  Sedelnikova, O A ^a   Karamychev, V N ^a   Panyutin, I G ^a   Neumann, R D ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DNA; IODINE 125; LIPOSOME; MULTIDRUG RESISTANCE PROTEIN 1; NUCLEAR LOCALIZATION SIGNAL PEPTIDE; NUCLEAR PROTEIN; OLIGONUCLEOTIDE; TRIPLEX FORMING OLIGONUCLEOTIDE; UNCLASSIFIED DRUG;

ARTICLE; CELL CULTURE; CELL SURVIVAL; CELLULAR DISTRIBUTION; CONCENTRATION RESPONSE; CONJUGATION; CONTROLLED STUDY; DNA PROBE; DNA STRAND BREAKAGE; GENE SEQUENCE; GENE TARGETING; GENE THERAPY; HUMAN; HUMAN CELL; INTRACELLULAR TRANSPORT; ISOTOPE LABELING; MAMMAL CELL; NUCLEAR LOCALIZATION SIGNAL; NUCLEOTIDE SEQUENCE; NUCLEOTIDE TRANSPORT; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; RESTRICTION MAPPING; SOUTHERN BLOTTING;

MAMMALIA;

EID: 0036236762     PISSN: 10872906     EISSN: None     Source Type: Journal    
DOI: 10.1089/108729002753670256     Document Type: Article

References (30)

1. Unambiguous demonstration of triple-helix-directed gene modification
2. Sequence-specific targeting and covalent modification of human genomic DNA
3. Triplex targeting of a native gene in permeabilized intact cells: Covalent modification of the gene for the chemokine receptor CCR5
4. Triplex DNA: Fundamentals, advances, and potential applications for gene therapy
5. Biological damage from the Auger effect, possible benefits
6. Intracellular disposition and metabolism of fluorescently-labeled unmodified and modified oligonucleotides microinjected into mammalian cells
7. Amplification of DNA sequences in human multidrug-resistant KB carcinoma cells
8. Triplex DNA structures
9. Analysis of oligonucleotide binding, internalization, and intracellular trafficking utilizing a novel radiolabeled crosslinker
10. Inhibition of gene expression by triple helix-directed DNA cross-linking at specific sites
11. The anti-gene strategy: Control of gene expression by triplex-forming-oligonucleotides
12. High-frequency intrachromosomal gene conversion induced by triplex-forming oligonucleotides microinjected into mouse cells
13. Targeted gene knockout mediated by triple helix forming oligonucleotides
14. A new family of sequence-specific DNA-cleaving agents directed by triple-helical structures: Benzopyridoindole-EDTA conjugates
15. Nucleocytoplasmic transport: The soluble phase
16. Cellular nucleic acid binding protein regulates the CT element of the human c-myc protooncogene
17. 125I labeled oligonucleotides
18. Sequence-specific DNA breaks produced by triplex-directed decay of iodine-125
19. 125I incorporated into a triplex-forming oligonucleotide correlates with geometry of the triplex
20. Development of DNA-based radiopharmaceuticals carrying Auger-electron emitters for anti-gene radiotherapy
21. 125I in a triplex-forming oligonucleotide
22. The human mdr1 (multidrug-resistance) gene harbours a long homopyrimidine-homopurine sequence next to a cluster of Alu repeated sequences in intron 14
23. Serum immunoglobulins interact with oligonucleotides
24. Radiotoxicity of iodine-125-labeled oligodeoxyribonucleotides in mammalian cells
25. The stability of DNA triplexes inside cells as studied by iodine-125 radioprinting
26. 125I-labeled triplex-forming oligonucleotides
27. Development of DNA-based radiopharmaceuticals carrying Auger-electron emitters for antigene radiotherapy
28. Triplex-directed modification of genes and gene activity
29. Gene delivery: A single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus