An ATP-dependent step is required for the translocation of microinjected precursor mRNA into nuclear speckles

Folia Biologica

Volumn 48, Issue 2, 2002, Pages 69-72

  Kopsky V ^a   Vecerova J ^a   Melcak I ^a   Pliss, A ^a   Stulik J ^a   Koberna, K ^a   Tomaskova L ^a   Raska I ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Czech Republic)

Author keywords

ATP dependent step; HeLa cell nucleus; Microinjected splicing competent RNA; RNA translocation into nuclear speckles

Indexed keywords

ADENOSINE TRIPHOSPHATE; MESSENGER RNA PRECURSOR; VIRUS RNA;

ADENOVIRUS; ARTICLE; CELL COMPARTMENTALIZATION; CELL NUCLEUS; CHROMATIN STRUCTURE; CONTROLLED STUDY; DIFFUSION; GENE TARGETING; HELA CELL; HUMAN; HUMAN CELL; MICROINJECTION; NONHUMAN; PROTEIN ASSEMBLY; RNA SPLICING; RNA TRANSPORT;

ADENOVIRIDAE;

EID: 0036234784     PISSN: 00155500     EISSN: 25337602     Source Type: Journal    
DOI: None     Document Type: Article

References (41)

1. Splicing of Balbiani ring 1 gene pre-mRNA occurs simultaneously with transcription
2. Protein components specifically associated with pre-spliceosome and spliceosome complexes
3. Splice site selection, rate of splicing, and alternative splicing on nascent transcripts
4. A CTD function linking transcription to splicing
5. Inefficient processing impairs release of RNA from the site of transcription
6. 97a Splicing factors associate with nuclear HCMV-IE transcripts after transcriptional activation of the gene, but dissociate upon transcription inhibition: Evidence for a dynamic organization of splicing factors
7. Perichromatin fibrils are in situ forms of nascent transcripts
8. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus
9. Simian virus 40 cRNA is processed into functional mRNA in microinjected monkey cells
10. Intron-dependent recruitment of pre-mRNA splicing factors to sites of transcription
11. Human cytomegalovirus immediate early interaction with host nuclear structures: Definition of an immediate transcript environment
12. Tracking COL1A1 RNA in osteogenesis imperfecta: Splice-defective transcripts initiate transport from the gene but are retained within the SC35 domain
13. Intron-dependent association of splicing factors with active genes
14. The structure and function of proteins involved in mammalian pre-mRNA splicing
15. Non-snRNP protein splicing factors
16. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription
17. Structural organization of the pre-mRNA splicing commitment. A hypothesis
18. Nuclear pre-mRNA compartmentalization: Trafficking of released transcripts to splicing factor reservoirs
19. Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles
20. A functional association between the 5′ and 3′ splice site is established in the earliest prespliceosome complex (E) in mammals
21. mRNA polyadenylation and its coupling to other RNA processing reactions and to transcription
22. Cell biology of transcription and pre-mRNA splicing: Nuclear architecture meets nuclear function
23. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo
24. The dynamics of a pre-mRNA splicing factor in living cells
25. Splicing of precursors to messenger RNAs by the spliceosome
26. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription
27. Small nuclear RNAs and pre-mRNA splicing
28. Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors
29. A minimal spliceosomal complex A recognizes the branch site and polypyrimidine tract
30. Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing
31. Processing of endogenous pre-mRNAs in association with SC-35 domains is gene specific
32. RNA polymerase II localizes at sites of human cytomegalovirus immediate-early RNA synthesis and processing
33. Higher order nuclear organization: Three-dimensional distribution of small nuclear ribonucleoprotein particles
34. Pre-mRNA processing and the CTD of RNA polymerase II: The tail that wags the dog?
35. Functions of the abundant U-snRNPs
36. Localization of pre-messenger RNA at discrete nuclear sites
37. Physical isolation of nascent RNA chains transcribed by RNA polymerase II: Evidence for cotranscriptional splicing
38. Higher level organization of individual gene transcription and RNA splicing: Integration of nuclear structure and function
39. Nonrandom gene organization: Structural arrangements of specific pre-mRNA transcription and splicing with SC-35 domains
40. Evidence for channeled diffusion of pre-mRNAs during nuclear RNA transport in metazoans
41. Localization of pre-mRNA splicing in mammalian nuclei