Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis

Phytochemistry

Volumn 54, Issue 7, 2000, Pages 657-666

  Warzecha, Heribert ^a   Gerasimenko, Irina ^a   Kutchan, Toni M ^b   Stöckigt, Joachim ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

^b LEIBNIZ INSTITUTE OF PLANT BIOCHEMISTRY   (Germany)

Author keywords

Apocynaceae; Heterologous expression; Plant cell suspension culture; Raucaffricine O D glucosidase; Rauvolfia serpentina; Substrate specificity

Indexed keywords

AJMALINE; ALKALOID; COMPLEMENTARY DNA; GLUCOSIDASE; INDOLE ALKALOID; PLANT GLYCOSIDE; RAUCAFFRICINE BETA GLUCOSIDASE; RAUCAFFRICINE BETA-GLUCOSIDASE; RAUWOLFIA ALKALOID; VOMILENINE;

AMINO ACID SEQUENCE; ANGIOSPERM; ARTICLE; BIOSYNTHESIS; CATALYSIS; CELL SUSPENSION; ENZYME ISOLATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; MOLECULAR CLONING; MOLECULAR GENETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT CELL; PROTEIN EXPRESSION; SOUTHERN BLOTTING;

ALKALOIDS; AMINO ACID SEQUENCE; ANGIOSPERMS; BASE SEQUENCE; BLOTTING, SOUTHERN; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GLUCOSIDASES; INDOLE ALKALOIDS; MOLECULAR SEQUENCE DATA; SECOLOGANIN TRYPTAMINE ALKALOIDS; SUBSTRATE SPECIFICITY;

APOCYNACEAE; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAGNOLIOPHYTA; RAUVOLFIA; RAUVOLFIA SERPENTINA;

EID: 0034705965     PISSN: 00319422     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0031-9422(00)00175-8     Document Type: Article

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