Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence

Molecular Microbiology

Volumn 43, Issue 4, 2002, Pages 869-881

  Bierne, Hélène ^a   Mazmanian, Sarkis K ^b,e   Trost, Matthias ^c   Pucciarelli, M Graciela ^d   Liu, Gwen ^b   Dehoux, Pierre ^a   Jänsch, Lothar ^c   Garcia del Portillo, Francisco ^f   Schneewind, Olaf ^b,e   Cossart, Pascale ^a  

^a INSTITUT PASTEUR   (France)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^d CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

^e UNIVERSITY OF CHICAGO   (United States)

^f NONE

Author keywords

[No Author keywords available]

Indexed keywords

AMIDINOTRANSFERASE; MEMBRANE PROTEIN; PEPTIDOGLYCAN; PROTEIN DERIVATIVE; PROTEIN INLA; SORTASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL CELL WALL; BACTERIAL COLONIZATION; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL VIRULENCE; BACTERIUM MUTANT; CELL ANCHORAGE; CELL LINE; CONTROLLED STUDY; EPITHELIUM CELL; FEMALE; GENE IDENTIFICATION; GENE INACTIVATION; GENETIC CODE; GENOME ANALYSIS; HUMAN; HUMAN CELL; INOCULATION; LISTERIA MONOCYTOGENES; LISTERIOSIS; LIVER; MASS SPECTROMETRY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN TARGETING; SPLEEN; SRTA GENE; SRTB GENE;

ANIMALIA; BACTERIA (MICROORGANISMS); LISTERIA; LISTERIA MONOCYTOGENES; POSIBACTERIA; PROKARYOTA;

EID: 0036226966     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2002.02798.x     Document Type: Article

References (44)

1. Iterated profile searches with PSI-BLAST - A tool for discovery in protein databases
2. Identification of new genes involved in the virulence of Listeria monocytogenes by signature-tagged transposon mutagenesis
3. Inactivation of the srtA gene in Streptococcus gordonii inhibits cell wall anchoring of surface proteins and decreases in vitro and in vivo adhesion
4. InlB: An invasion protein of Listeria monocytogenes with a novel type of surface association
5. The 213-amino-acid leucine-rich repeat region of the Listeria monocytogenes InlB protein is sufficient for entry into mammalian cells, stimulation of PI 3-kinase and membrane ruffling
6. Dual roles of plcA. Listeria monocytogenes pathogenesis
7. The use of host cell machinery in the pathogenesis of Listeria monocytogenes
8. Sortase, a universal target for therapeutic agents against Gram- positive bacteria?
9. Interactions of Listeria monocytogenes with mammalian cells during entry and actin-based movement: Bacterial factors, cellular ligands and signaling
10. MATCH-BOX: A fundamentally new algorithm for the simultaneous alignment of several protein sequences
11. Anchor structure of cell wall surface proteins in Listeria monocytogenes
12. Entry of Listeria monocytogenes into hepatocytes requires expression of InlB, a surface protein of the internalin multigene family
13. Identification of four new members of the internalin multigene family ofListeria monocytogenes EGD
14. Conservation of a hexapeptide sequence in the anchor region of surface proteins from gram-positive cocci
15. Molecular cloning and expression of internalin in Listeria
16. Comparative genomics of Listeria species
17. Separation and quantification of muropeptides with high-performance liquid chromatography
18. A role for phosphoinositide 3-kinase in bacterial invasion
19. Interaction between the protein InlB of L. monocytogenes and lipoteichoic acid: A novel mechanism of protein association at the surface of Gram positive bacteria
20. The complete genome sequence of the gram-positive bacterium Bacillus subtilis
21. Whole genome sequencing of meticillin-resistant Staphylococcus aureus
22. Internalin must be on the bacterial surface to mediate entry of Listeria monocytogenes into epithelial cells
23. A single amino acid in E-cadherin responsible for host specificity towards the human pathogen Listeria monocytogenes
24. Internalin of Listeria monocytogenes with an intact leucine-rich repeat region is sufficient to promote internalization
25. A transgenic model for listeriosis: Role of internalin in crossing the intestinal barrier
26. Expression of the Listeria monocytogenes EGD inlA and inlB genes, whose products mediate bacterial entry into tissue culture cell lines, by PrfA-dependent and -independent mechanisms
27. Modified Listeria bacteriophage lysin genes (ply) allow efficient overexpression and one-step purification of biochemically active fusion proteins
28. Cell wall anchored surface proteins and lipoproteins of gram-positive bacteria
29. Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
30. Staphylococcus aureus sortase mutants defective in the display of surface proteins and in the pathogenesis of animal
31. Sortase-catalalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus
32. Antibodies to the leucine-rich repeat region of internalin block entry of Listeria monocytogenes into cells expressing E-cadherin
33. E-cadherin is the receptor for internalin, a surface protein required for entry of Listeria monocytogenes into epithelial cells
34. Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in Gram-positive bacteria
35. Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope
36. An embarrassment of sortases - A richness of substrates?
37. The gene cluster inlC2DE of Listeria monocytogenes contains additional new internalin genes and is important for virulence in mice
38. A Gly145Ser substitution in the transcriptional activator PrfA causes constitutive over-expression of virulence factors in Listeria monocytogenes
39. Cell wall sorting signals in surface proteins of gram-positive bacteria
40. Internalins from the human pathogen Listeria monocytogenes combine three distinct folds into a contiguous internalin domain
41. A workbench for multiple alignment construction and analysis
42. Isolation of Listeria monocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread
43. Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif
44. Anchoring of surface proteins to the cell wall of Staphylococcus aureus. Sortase catalyzed in vitro transpeptidation reaction using LPXTG peptide and NH(2)-Gly(3) substrates