Ultrasensitive pharmacological characterisation of the voltage-gated potassium channel KV1.3 studied by single-molecule fluorescence microscopy

Histochemistry and Cell Biology

Volumn 117, Issue 3, 2002, Pages 197-202

  Freudenthaler, Günter ^a   Axmann, Markus ^a   Schindler, Hansgeorg ^a   Pragl, Bernt ^b   Knaus, Hans Günther ^b   Schütz, Gerhard J ^a  

^a JOHANNES KEPLER UNIVERSITY LINZ   (Austria)

^b UNIVERSITY OF INNSBRUCK   (Austria)

Author keywords

Fluorescence microscopy; Hongotoxin; Jurkat cells; Pharmacology; Single molecule microscopy; Voltage gated potassium channel KV1.3

Indexed keywords

FLUORESCENT DYE; PEPTIDE; POTASSIUM CHANNEL; RADIOLIGAND; TOXIN; VENOM;

ARTICLE; CHANNEL GATING; CONTROLLED STUDY; DISSOCIATION CONSTANT; FIBROBLAST; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; LIGAND BINDING; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN BINDING; RADIOIODINATION; SCORPION; STOICHIOMETRY; VALIDATION PROCESS;

BINDING, COMPETITIVE; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; HUMANS; IODINE RADIOISOTOPES; JURKAT CELLS; KV1.3 POTASSIUM CHANNEL; MICROSCOPY, FLUORESCENCE; MUTATION; NEUROTOXINS; POTASSIUM CHANNELS; POTASSIUM CHANNELS, VOLTAGE-GATED; RADIOLIGAND ASSAY; SCORPION VENOMS; TRANSFECTION;

CENTRUROIDES; CENTRUROIDES LIMBATUS; SCORPIONES;

EID: 0036210605     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00418-001-0374-y     Document Type: Article

References (32)

1. + channels. Effects of channel gating, voltage, and ionic strength
2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
3. Probing single molecules in single living cells
4. On/off blinking and switching behaviour of single molecules of green fluorescent protein
5. Membrane specific mapping and colocalization of malarial and host skeletal proteins in the Plasmodium falciparum infected erythrocyte by dual-color near-field scanning optical microscopy
6. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution
7. + channel
8. Single molecule dynamics studied by polarization modulation
9. Probing the interaction between two single molecules: Fluorescence resonance energy transfer between a single donor and a single acceptor
10. Hindered rotational diffusion and rotational jumps of single molecules
11. Single-molecule anisotropy imaging
12. Single-molecule imaging of l-type Ca(2+) channels in live cells
13. Margatoxin binds to a homomultimer of K(V)1.3 channels in Jurkat cells. Comparison with K(V)1.3 expressed in CHO cells
14. Single molecule imaging of green fluorescent proteins in living cells: E-cadherin forms oligomers on the free cell surface
15. Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin
16. Patterning proteins and cells using soft lithography
17. Subunit composition of brain voltage-gated potassium channels determined by hongotoxin-1, a novel peptide derived from Centruroides limbatus venom
18. Single-molecule enzymatic dynamics
19. + channel receptor
20. + channel-blocking peptides
21. Illuminating single molecules in condensed matter
22. Assembly and suppression of endogenous Kv1.3 channels in human T cells
23. + channels
24. Charybdotoxin inhibits proliferation and interleukin 2 production in human peripheral blood lymphocytes
25. Single-molecule imaging of EGFR signalling on the surface of living cells
26. Imaging of single molecule diffusion
27. Single-molecule microscopy on model membranes reveals anomalous diffusion
28. Direct observation of ligand colocalization on individual receptor molecules
29. 3D mapping of individual ion channels on living cells
30. Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy
31. 3D imaging of individual ion channels in live cells at 40 nm resolution
32. Single molecule microscopy of biomembranes