Methanol determination in Pichia pastoris cultures by flow injection analysis

Biotechnology Letters

Volumn 24, Issue 5, 2002, Pages 413-417

  Almuzara, C ^a   Cos, O ^b   Baeza, M ^a   Gabriel, D ^b   Valero, F ^b  

^a Escola Universitaria Politecnica del Medi Ambient   (Spain)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Alcohol oxidase; Flow injection analysis; Methanol; Peroxidase; Pichia pastoris

Indexed keywords

ALCOHOL OXIDASE; METHANOL; PEROXIDASE;

ARTICLE; AUTOMATION; BACTERIAL GROWTH; BIOMASS; CHEMICAL ANALYSIS; CONCENTRATION (PARAMETERS); ENZYME MECHANISM; FLOW INJECTION ANALYSIS; NONHUMAN; PICHIA PASTORIS; PROTEIN SYNTHESIS;

BACTERIA (MICROORGANISMS); PICHIA; PICHIA PASTORIS;

EID: 0036203469     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1014554324117     Document Type: Article

References (11)

1. Ion-selective electrodes responsive to anionic surfactants for flow injection analysis
2. Selective flow-injection determination of methanol using immobilized enzyme reactors
3. On-line monitoring and control of methanol concentration in shake-flask cultures of Pichia pastoris
4. Heterologous protein expression in the methylotrophic yeast Pichia pastoris
5. Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris
6. Colorimetric method for the determination of ethanol by flow injection analysis
7. Selective flow-injection determination of methanol in the presence of ethanol based on a multi-enzyme system with chemiluminiscence determination
8. Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris
9. Automation of selective assays for on-line bioprocess monitoring by flow-injection analysis
10. Modeling Pichia pastoris growth on methanol and optimising the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, Serotype A