Acetaminophen hepatotoxicity and mechanisms of its protection by N-acetylcysteine: A study of Hep3B cells

Experimental and Toxicologic Pathology

Volumn 53, Issue 6, 2002, Pages 489-500

  Manov, Irena ^a   Hirsh, Mark ^a   Iancu, Theodore C ^a  

^a TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

Acetaminophen; Apoptosis; Electron microscopy; Hep3B cultured cells; Hepatotoxicity; N acetylcysteine; Oxidative stress; Reactive oxygen species

Indexed keywords

ACETYLCYSTEINE; GLUTATHIONE; LACTATE DEHYDROGENASE; PARACETAMOL; REACTIVE OXYGEN METABOLITE;

APOPTOSIS; ARTICLE; CELL CULTURE; CELL LEVEL; CELL MEMBRANE PERMEABILITY; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DEPLETION; DRUG CYTOTOXICITY; DRUG EFFECT; DRUG EXPOSURE; ENZYME RELEASE; FLOW CYTOMETRY; HEPATOMA CELL; HUMAN; HUMAN CELL; LIVER PROTECTION; LIVER TOXICITY; OXIDATIVE STRESS; PATHOPHYSIOLOGY; TRANSMISSION ELECTRON MICROSCOPY;

EID: 0036197307     PISSN: 09402993     EISSN: None     Source Type: Journal    
DOI: 10.1078/0940-2993-00215     Document Type: Article

References (49)

1. Oxidative stress in cultured hepatocytes exposed to acetaminophen
2. The role of DT-diaphorase in the maintenance of the reduced antioxidant form of coenzyme Q in membrane systems
3. Cytotoxicity of acetaminophen in human cytochrome P4502E1 - Transfected HepG2 cells
4. Inhibition of mitochondrial respiration in vivo is an early event in acetaminophen-induced hepatotoxicity
5. N-acetyl-L-cysteine inhibits apoptosis in human male germ cells in vitro
6. Reversible and irreversible inhibition of hepatic mitochondrial respiration by acetaminophen and its toxic metabolite, N-acetyl-p-benzoquinoneimine (NAPQI)
7. Role of nitric oxide in acetaminophen-induced hepatotoxicity in the rat
8. Mechanism of acetaminophen-induced hepatotoxicity: Covalent binding versus oxidative stress
9. Characterization of N-acetylcysteine and ambroxol in anti-oxidant therapy
10. The regulation expression of erythropoietin by two human hepatoma cell lines
11. Age-related toxicity of paracetamol in mouse hepatocytes
12. Therapeutic misadventures with acetaminophen: Hepatotoxicity after multiple doses in children
13. Acetaminophen metabolism and cytotoxicity in PC12 cells transfected with cytochrome P4502E1
14. Blockage of apoptotic signaling of transforming growth factor-β in human hepatoma cells by carboxyfullerene
15. Effects of N-acetyl-L-cysteine on T-cell apoptosis are not mediated by increased cellular glutathione
16. Mechanism of action and value of N-acetylcysteine in the treatment of early and late acetaminophen poisoning: A critical review
17. Effect of antioxidants, resveratrol, quercetin, and N-acetylcysteine, on the functions of cultured rat hepatic stellate cells and Kupffer cells
18. Acetaminophen intoxication during treatment: What you don't know can hurt you
19. Clinical application of N-acetylcysteine
20. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen
21. Evaluation of the probe 2′,7′-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress
22. Role of mitochondria in oxidative stress and ageing
23. Mechanism of protection of ebselen against paracetamol-induced toxicity in rat hepatocytes
24. Oxidative stress by acute acetaminophen administration in mouse liver
25. Mechanisms of liver damage
26. Protein measurement with the folin phenol reagent
27. Contribution of CYP2E1 and CYP3A to acetaminophen reactive metabolite formation
28. 2+-mediated endonuclease activation
29. Spontaneous chemiluminiescence production, lipid peroxidation, and covalent binding in rat hepatocytes exposed to acetaminophen
30. Assessment of the Alamar blue assay for cellular growth and viability in vitro
31. Acetaminophen administration alters rate of cell replication in hepatoma cells without activation of drugs by cytochrome P450
32. Rifampicin and isoniazid increase acetaminophen and isoniazid cytotoxicity in human HepG2 hepatoma cells
33. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry
34. Hepatotoxicity of mild analgesics
35. Covalent binding of xenobiotics to specific proteins in the liver
36. 2+ in the nucleus during acetaminophen-induced liver injury
37. 2+ antagonists inhibit DNA fragmentation and toxic cell death induced by acetaminophen
38. Protection of acetaminophen-induced hepatocellular apoptosis and necrosis by cholesteryl hemisuccinate pretreatment
39. A novel proanthocyanidin IH636 grape seed extract increases in vivo BCL-XL expression and prevents acetaminophen-induced programmed and unprogrammed cell death in mouse liver
40. Effect of N-acetylcysteine and dithiothreitol on glutathione and protein thiol replenishment during acetaminophen-induced toxicity in isolated mouse hepatocytes
41. Calcium-dependent DNA damages and adenosine 3′,5′-cyclic monophosphate-independent glycogen phosphorylase activation in an in vitro model of acetaminophen-induced liver injure
42. 2+ in the MCF-10A human mammary cell line by carcinogenic polycyclic aromatic hydrocarbons
43. Enzymatic method for quantitative determination of nanogram amounts of total and oxidased glutathione: Application to mammalian blood and other tissue
44. Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells
45. A microtiter plate assay for total glutathione and glutathione disulfide contents in cultured/isolated cells: Performance study of a new miniaturized protocol
46. Inhibitory effect of N-acetylcysteine on superoxide anion generation in human polymorphonuclear leukocytes
47. +-ATPase to hypertrophy and its marker genes in cardiac myocytes
48. Acetaminophen (paracetamol) hepatotoxicity with regular intake of alcohol: Analysis of instances of therapeutic misadventure