Triple immunofluorescence staining with antibodies raised in the same species to study the complex innervation pattern of intrapulmonary chemoreceptors

Journal of Histochemistry and Cytochemistry

Volumn 50, Issue 4, 2002, Pages 575-582

  Brouns, Inge ^a   Van Nassauw, Luc ^a   Van Genechten, Jeroen ^a   Majewski, Mariusz ^a   Scheuermann, Dietrich W ^a   Timmermans, Jean Pierre ^a   Adriaensen, Dirk ^a  

^a UNIVERSITY OF ANTWERP   (Belgium)

Author keywords

Confocal laser scanning microscopy; Fab secondary antibodies; Multiple immunostaining; Pulmonary neuroepithelial bodies; Same species; Triple immunostaining; Tyramide signal amplification

Indexed keywords

ANTIBODY; CALBINDIN; CALCITONIN GENE RELATED PEPTIDE; IMMUNOGLOBULIN F(AB) FRAGMENT; ISOPROPAMIDE IODIDE; NITRIC OXIDE SYNTHASE;

ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; CHEMORECEPTOR; IMMUNOCYTOCHEMISTRY; IMMUNODETECTION; IMMUNOFLUORESCENCE; LUNG; NONHUMAN; PRIORITY JOURNAL; RAT; STAINING;

ANIMALIA;

EID: 0036193803     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540205000415     Document Type: Article

References (27)

1. Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains
2. Selective innervation of neuroepithelial bodies by intrinsic pulmonary nitrergic neurons
3. Neuroendocrine cells and nerves of the lung
4. Pulmonary intraepithelial vagal nodose afferent nerve terminals are confined to neuroepithelial bodies. An anterograde tracing and confocal microscopy study in adult rats
5. Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays
6. 3 receptors
7. Enhanced immunocytochemical detection of a yet unreported intrinsic nitrergic component of the innervation of pulmonary neuroepithelial bodies
8. Occurrence, distribution and ontogeny of CGRP immunoreactivity in the rat lower respiratory tract: Effect of capsaicin treatment and surgical denervations
9. Neuroepithelial bodies as airway oxygen sensors
10. Heine oxygenase immunoreactive neurons in the rat intestine and their relationship to nitrergic neurons
11. Quadruple immunofluorescence: A direct visualization method
12. Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining
13. A highly sensitive detection method for immunohistochemistry using biotinylated tyramine
14. A specific double-immunofluorescence method for the demonstration of S-antigen and serotonin in trout and rat pinealocytes by means of primary antibodies from the same donor species
15. Immunocytochemistry
16. An indirect immunofluorescence procedure for staining the same cryostat section with two mouse monoclonal primary antibodies
17. Light and electron microscopic immunocytochemistry of the same nerves from whole mount preparations
18. F(ab) secondary antibodies: A general method for double immunolabeling with primary antisera from the same species. Efficiency control by chemiluminescence
19. Applications of double-label immunofluorescence
20. Pulmonary calcitonin gene-related peptide immunoreactivity: Nerve-endocrine cell interrelationships
21. Double immunofluorescent staining using two unconjugated primary antisera raised in the same species
22. Neuroepithelial bodies and solitary small-granule cells
23. Ontogeny of neuroepithelial bodies: Correlations with mitogenesis and innervation
24. Delineation of human thymocyte differentiation pathways utilizing double staining techniques with monoclonal antibodies
25. Microwaving for double indirect immunofluorescence with primary antibodies from the same species and for staining of mouse tissues with mouse monoclonal antibodies
26. Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition
27. Simultaneous demonstration of multiple antigens by indirect immunofluorescence or immungold staining: Novel light and electron microscopical double- and triple-staining method employing primary antibodies from the same species