Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining

Journal of Histochemistry and Cytochemistry

Volumn 44, Issue 12, 1996, Pages 1353-1362

  Hunyady, Béla ^a,c   Krempels, Krisztina ^b   Harta, Gyöngyi ^a   Mezey, Éva ^b  

^a NATIONAL INSTITUTE OF MENTAL HEALTH   (United States)

^b NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE   (United States)

^c UNIVERSITY OF PÉCS   (Hungary)

Author keywords

ABC technique; Amplification; Glucagon; Hypothalamus; Immunofluorescence technique; Immunohistochemistry; Neurofilament; Oxytocin; Pancreas; Pancreatic polypeptide; Proton pump; Rat; Stomach; Tyramine conjugates; Tyrosine hydroxylase

Indexed keywords

BIOTIN; FLUOROCHROME; GLUCAGON; HORSERADISH PEROXIDASE; MONOCLONAL ANTIBODY; OXYTOCIN; POLYCLONAL ANTIBODY; TYRAMINE; TYROSINE 3 MONOOXYGENASE;

ANIMAL TISSUE; ANTIGEN DETECTION; ARTICLE; CONTROLLED STUDY; GENE AMPLIFICATION; HYPOTHALAMUS; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; MALE; NEUROFILAMENT; NONHUMAN; PANCREAS; PRIORITY JOURNAL; RAT; STOMACH; TISSUE FIXATION; TISSUE SECTION;

EID: 3242829141     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/44.12.8985127     Document Type: Article

References (16)

1. Adams JC (1992) Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains. J Histochem Cytochem 40:1437-1463
2. Adrian TE, Bloom SR, Bryant MG, Polak JM, Heitz PH, Barnes AJ (1976) Distribution and release of human pancreatic polypeptide. Gut 17:940-944
3. Alstein M, Whitnall MH, House S, Key S, Gainer H (1988) An immunochemical analysis of oxytocin and vasopressin prohormone processing in vivo. Peptides 9:87-105
4. Beitner-Johnson D, Guitart X, Neslter EJ (1992) Neurofilament proteins and the mesolimbic dopamine system: common regulation by chronic morphine and chronic cocaine in the rat ventral tegmental area. J Neurosci 12:2165-2176
5. Berghorn KA, Bonnett JH, Hoffman GE (1994) cFos immunoreactivity is enhanced with biotin amplification. J Histochem Cytochem 42:1635-1642
6. Bobrow MN, Harris TD, Shaughnessy KJ, Litt GJ (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J Immunol Methods 125:279-285
7. Franzusoff A, Redding K, Crosby J, Fuller RS, Schekman R (1991) Localization of components involved in protein transport and processing through the yeast Golgi apparatus. J Cell Biol 112:27-37
8. Hsu SM, Raine L, Fanger H (1981) A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol 75:734-738
9. Hunyady B, Mezey É, Pacak K, Palkovits M (1996) Identification of endogenous peroxidase containing cells as eosinophils in the gastrointestinal system. Histochemistry, in press
10. Kiss A, Palkovits M, Skirboll LR (1988) Light microscopic triple-colored immunohistochemical staining on the same vibratome section using the avidinbiotin-peroxidase complex technique. Histochemistry 88:353-356
11. Lewis Carl SA, Gillete-Ferguson I, Ferguson DG (1993) An indirect immunofluorescence procedure for staining the same cryosection with two mouse monoclonal primary antibodies. J Histochem Cytochem 41:1273-1278
12. Negoescu A, Labat-Moleur F, Lorimier P, Lamarcq L, Guillermet C, Chambaz E, Brambilla E (1994) F(ab) secondary antibodies: a general method for double immunolabeling with primary antisera from the same species. Efficiency control by chemiluminescence. J Histochem Cytochem 42:433-437
13. Petrusz P, Ordronneau P, Finley JC (1980) Criteria of reliability for light microscopic immunocytochemical staining. Histochem J 12:333-348
14. Reddy SN, Bibby NJ, Elliott RB (1985) Cellular distribution of insulin, glucagon, pancreatic polypeptide hormone and somatostatin in the fetal and adult pancreas of the guinea pig: a comparative immunohistochemical study. Eur J Cell Biol 38:301-305
15. Smolka A, Swiger KM (1992) Site-directed antibodies as topographical probes of the gastric H,K-ATPase alpha-subunit. Biochim Biophys Acta 1108:75-85
16. Wessel GM, McClay DR (1986) Two embryonic, tissue-specific molecules identified by a double-label immunofluorescence technique for monoclonal antibodies. J Histochem Cytochem 34:703-706