Real-time kinetic analyses of the interaction of ricin toxin A-chain with ribosomes prove a conformational change involved in complex formation

Journal of Biochemistry

Volumn 131, Issue 2, 2002, Pages 267-275

  Honjo, Eijiro ^a   Watanabe, Keiichi ^a   Tsukamoto, Takuji ^a  

^a SAGA UNIVERSITY   (Japan)

Author keywords

BIAcore; Ribosome inactivating protein; Ricin A chain; RNA N glycosidase; Surface plasmon resonance

Indexed keywords

ADENINE; DEXTRAN; RIBOSOME INACTIVATING PROTEIN; RICIN; RICIN TOXIN A; UNCLASSIFIED DRUG;

ARTICLE; BIOSENSOR; COMPLEX FORMATION; ENZYME ACTIVITY; EUKARYOTE; GEL; IONIC STRENGTH; NONHUMAN; PROKARYOTE; PROTEIN BINDING; PROTEIN INTERACTION; RIBOSOME; RICINUS COMMUNIS; SURFACE PLASMON RESONANCE;

CASTOR; ESCHERICHIA COLI; EUKARYOTA; PHASEOLUS (ANGIOSPERM); PROKARYOTA; RICINUS COMMUNIS;

EID: 0036185561     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003098     Document Type: Article

References (36)

1. Ricin: Structure, mode of action, and some current applications
2. RNA N-glycosidase activity of ricin A-chain. Mechanism of action of the toxin lectin ricin on eukaryotic ribosomes
3. The conformation of the sarcin/ricin loop from 28S ribosomal RNA
4. The sarcin/ricin loop, a modular RNA
5. Crystal structure of the ribosomal RNA domain essential for binding elongation factors
6. Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23 S RNA
7. Ribosome-inactivating proteins from plants
8. The structure and function of ribosome-inactivating proteins
9. X-ray analysis of substrate analogs in the ricin A-chain active site
10. Binding of ricin A chain to rat liver ribosomes: Relationship to ribosome inactivation
11. Kinetic analysis of macromolecular interactions using surface plasmon resonance biosensors
12. Use of surface plasmon resonance to probe the equilibrium and dynamic aspects of interactions between biological macromolecules
13. BIAcore for macromolecular interactions
14. A novel hydrogel matrix on gold surfaces in surface plasmon resonance sensors for fast and efficient covalent immobilization of ligands
15. Genomic cloning and characterization of a ricin gene from Ricinus communis
16. Protein measurement with the folin phenol reagent
17. Dissociation of mammalian polyribosomes into subunits by puromycin
18. Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors
19. Immobilization chemistries suitable for use in the BIAcore surface plasmon resonance detector
20. Quantitative determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins
21. Concentration measurement of unpurified proteins using biosensor technology under conditions of partial mass transport limitation
22. Theoretical analysis of protein concentration determination using biosensor technology under conditions of partial mass transport limitation
23. Antigen-antibody binding and mass transport by convection and diffusion to a surface: A two dimensional computer model of binding and dissociation kinetics
24. The structure and function of eukaryotic ribosomes
25. Physicochemical characterization of ribosomal particles from the eukaryote Artemia
26. Extending the range of rate constants available from BIACORE: Interpreting mass transport-influenced binding data
27. Fluorescence studies on the interaction of adenine with ricin A-chain
28. Analysis of mass transport-limited binding kinetics in evanescent wave biosensors
29. Kinetic and concentration analysis using BIA technology
30. Kinetics of ligand binding to receptor immobilized in a polymer matrix, as detected with an evanescent wave biosensor. 1. A computer simulation of the influence of mass transport
31. X-ray structure of recombinant ricin A-chain at 1.8 Å resolution
32. Ricin A-chain structural determinant for binding substrate analogues: A molecular dynamics simulation analysis
33. Ribosome-mediated folding of partially unfolded ricin A-chain
34. Major structural differences between pokeweed antiviral protein and ricin A-chain do not account for their differing ribosome specificity
35. The two faces of the Escherichia coli 23 S rRNA sarcin/ricin domain: The structure at 1.11 Å resolution
36. Pokeweed antiviral protein accesses ribosomes by binding to L3