Concentration measurement of unpurified proteins using biosensor technology under conditions of partial mass transport limitation

Analytical Biochemistry

Volumn 249, Issue 2, 1997, Pages 165-173

  Richalet Sécordel, Pascale M ^a   Rauffer Bruyère, Nathalie ^a   Christensen, Lars L H ^b   Ofenloch Haehnle, Beatus ^c   Seidel, Christoph ^c   Van Regenmortel, Marc H V ^a  

^a INSTITUT DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^c F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSENSORS; ESCHERICHIA COLI; LIGANDS; MONOCLONAL ANTIBODIES; RATE CONSTANTS;

ASSOCIATION RATE CONSTANTS; CONCENTRATION MEASUREMENT; CONTINUOUS FLOWS; INTRINSIC BINDING; MASS TRANSPORT LIMITATION; PAPAIN CLEAVAGE; PROTEIN CONCENTRATIONS; SENSOR SURFACES;

FLOW RATE;

IMMUNOGLOBULIN F(AB) FRAGMENT;

ARTICLE; BINDING AFFINITY; BIOSENSOR; DIFFUSION COEFFICIENT; ESCHERICHIA COLI; FLOW MEASUREMENT; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN PURIFICATION; TECHNOLOGY; TRANSPORT KINETICS;

ESCHERICHIA COLI;

EID: 0031194210     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1997.2183     Document Type: Article

References (20)

1. Brooks, S. P. J., Lampi, B. J., Sarwar, G., and Botting, H. G. (1995) Anal. Biochem. 226, 26-30.
2. Johnsson, B., Löfas, S., and Lindquist, G. (1991) Anal. Biochem. 198, 268-277.
3. Fägerstam, L. G., Frostell-Karlsson, A., Karlsson, R., Persson, B., and Rönnberg, I. (1992) J. Chromatogr. 597, 397-410.
4. Glaser, R. W. (1993) Anal. Biochem. 213, 152-161.
5. Karlsson, R., Fägerstam, L., Nilshans, H., and Persson, B. (1993) J. Immunol. Methods 166, 75-84.
6. Christensen, L. H. (1997) Anal. Biochem. 249, 153-164.
7. Matsushita, S., Robert-Guroff, M., Rusche, J., Koito, A., Hattori, T., Hoshino, H., Javaherian, K., Takatsuki, K., and Putney, S. (1988) J. Virol. 62, 2107-2114.
8. Hoess, E., Huebner-Parajsz, C., Borgya, A., Faatz, E., Gröl, M., and Ofenloch-Haehnle, B. (1996) in XI International Conference on AIDS, pp. 256, Vancouver.
9. Al Moudallal, Z., Briand, J. P., and Van Regenmortel, M. H. V. (1985) EMBO J. 4, 1231-1235.
10. Chatellier, J., Rauffer-Bruyere, N., Van Regenmortel, M. H. V., Weiss, E., and Altschuh, D. (1996) J. Mol. Recogn. 9, 39-51.
11. Atherton, E., and Sheppard, R. C. (1989) Solid Phase Peptide Synthesis - A Practical Approach, Oxford Univ. Press, Oxford.
12. Seidel, C., Klein, C., Empl, E., Bayer, H., Lin, M., and Batz, H.-G. (1990) in Peptides 1990 (Giralt, E., and Andreu, D., Eds.), pp. 236-237, Leiden, The Neitherlands.
13. Altschuh, D., Dubs, M.-C., Weiss, E., Zeder-Lutz, G., and Van Regenmortel, M. H. V. (1992) Biochemistry 31, 6298-6304.
14. Gerin, J. L., Holland, P. V., and Purcell, R. H. (1971) J. Virol. 7, 569-576.
15. Azimzadeh, A., Pellequer, J. L., and Van Regenmortel, M. H. V. (1992) J. Mol. Recogn. 5, 9-18.
16. Karlsson, R., Roos, H., Fägerstam, L., and Persson, B. (1994) Methods Companion Methods Enzymol. 6, 99-110.
17. Schuck, P. (1996) Biophys. J., 1230-1249.
18. Schuck, P., and Milton, A. P. (1996) Anal. Biochem., 262-272.
19. Karlsson, R., and Fait, A. (1997) J. Immunol. Methods 200, 121-133.
20. O'Shannessy, D. J., Brigham-Burke, M., Soneson, K. K., Hensley, P., and Brooks, I. (1993) Anal. Biochem. 212, 457-468.