Ty3 integrase is required for initiation of reverse transcription

Journal of Virology

Volumn 76, Issue 6, 2002, Pages 2804-2816

  Nymark McMahon, M Henrietta ^a   Beliakova Bethell, Nadejda S ^a   Darlix, Jean Luc ^a   Le Grice, Stuart F J ^a   Sandmeyer, Suzanne B ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DNA POLYMERASE; HYBRID PROTEIN; INTEGRASE; RNA DIRECTED DNA POLYMERASE; VIRUS PROTEIN; ZINC;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL LEVEL; DNA INTEGRATION; DNA TRANSPOSITION; ENZYME ACTIVE SITE; ENZYME STRUCTURE; GENE MUTATION; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; RETROVIRUS; REVERSE TRANSCRIPTION; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION INITIATION; VIRUS CAPSID; VIRUS PARTICLE;

DNA, COMPLEMENTARY; DNA, VIRAL; INTEGRASES; MUTATION; RETROELEMENTS; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION, GENETIC; VIRION; VIRUS ASSEMBLY;

EID: 0036184671     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.76.6.2804-2816.2002     Document Type: Article

References (59)

1. Ribosome-mediated incorporation of a non-standard amino acid into a peptide through expansion of the genetic code
2. RNA packaging
3. HIV-1 infection requires a functional integrase NLS
4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding
5. Mutations in HIV reverse transcriptase which alter RNase H activity and decrease strand transfer efficiency are suppressed by HIV nucleocapsid protein
6. Coupled integration of human immunodeficiency virus type 1 cDNA ends by purified integrase in vitro: Stimulation by the viral nucleocapsid protein
7. Ty3 integrates within the region of RNA polymerase III transcription initiation
8. A yeast sigma composite element, Ty3, has properties of a retrotransposon
9. Amino acid sequence analysis of reverse transcriptase subunits from avian myeloblastosis virus
10. Characterization of active reverse transcriptase and nucleoprotein complexes of the yeast retrotransposon Ty3 in vitro
11. Nucleocapsid protein of human immunodeficiency virus as a model protein with chaperoning functions and as a target for antiviral drugs
12. Analytical study of avian reticuloendotheliosis virus dimeric RNA generated in vivo and in vitro
13. Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLVIII/LAV
14. Evidence of interactions between the nucleocapsid protein NCp7 and the reverse transcriptase of HIV-1
15. Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex
16. Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication
17. Structure-based mutagenesis of the catalytic domain of human immunodeficiency virus type 1 integrase
18. The genomic RNA in Ty1 virus-like particles is dimeric
19. Complementation of integrase function in HIV-1 virions
20. Characterization of human immunodeficiency virus type 1 dimeric RNA from wild-type and protease-defective virions
21. Maturation of dimeric viral RNA of Moloney murinc leukemia virus
22. The yeast Ty3 retrotransposon contains a 5′/3′ bipartite primer binding site and encodes nucleocapsid protein NCp9 functionally homologous to HIV-1 NCp7
23. HIV-1 infection of non-dividing cells through the recognition of integrase by the importin/karyopherin pathway
24. Sequence-specific DNA binding by Ku autoantigen and its effects on transcription
25. Structural characterization of the avian retrovirus reverse transcriptase and endonuclease domains
26. Evidence for the packaging of multiple copies of Tf1 mRNA into particles and the trans priming of reverse transcription
27. Ty3 GAG3 and POL3 genes encode the components of intracellular particles
28. Ty3, a yeast retrotransposon associated with tRNA genes, has homology to animal retroviruses
29. Characterization of a transpositionally active Ty3 element and identification of the Ty3 integrase protein
30. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli
31. Purification of the DNA polymerase of avian myeloblastosis virus
32. Ty3 transposes in mating populations of yeast: A novel transposition assay for Ty3
33. Proteolytic processing of Ty3 proteins is required for transposition
34. Ty3 integrase mutants defective in reverse transcription or 3′ end processing of extrachromosomal Ty3 DNA
35. Characterization of the nuclear localization signal in the avian sarcoma virus integrase
36. Rapid and efficient site-specific mutagenesis without phenotypic selection
37. Moloney murine leukemia virus integrase protein augments viral DNA synthesis in infected cells
38. Human immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to integrate viral DNA efficiently during infection
39. Involvement of HIV-I nucleocapsid protein in the recruitment of reverse transcriptase into nucleoprotein complexes formed in vitro
40. Integrase mediates nuclear localization of ty3
41. HIV-1 reverse transcriptase: Crystallization and analysis of domain structure by limited proteolysis
42. Genetic analysis of human immunodeficiency virus type 1 integrase and the U3 att site: Unusual phenotype of mutants in the zinc finger-like domain
43. Transposition of the yeast retroviruslike element Ty3 is dependent on the cell cycle
44. Mutations in non-conserved domains of Ty3 integrase affect multiple stages of the Ty3 life cycle
45. Interaction of p55 reverse transcriptase from the Saccharomyces cerevisiae retrotransposon Ty3 with conformationally distinct nucleic acid duplexes
46. Morphogenesis at the retrotransposon-retrovirus interface: Gypsy and Copia families in yeast and Drosophila
47. Virus-coded origin of a 32,000-dalton protein from avian retrovirus cores:structural relatedness of p32 and the beta polypeptide of the avian retrovirus DNA polymerase
48. Endonuclease activity associated with reverse transcriptase of avian sarcoma-leukosis viruses
49. Synthesis, assembly, and processing of retroviral proteins
50. Identification of critical amino acid residues in human immunodeficiency virus type 1 required for efficient proviral DNA formation at steps prior to integration in dividing and nondividing cells
51. Soluble Rous sarcoma virus reverse transcriptases alpha, alphabeta, and beta purified from insect cells are processive DNA polymerases that lack an RNase H 3′ → 5′ directed processing activity
52. Asymmetric subunit organization of heterodimeric Rous sarcoma virus reverse transcriptase alphabeta: Localization of the polymerase and RNase H active sites in the alpha subunit
53. Replication of avian leukosis viruses with mutations at the primer binding site: Use of alternative tRNAs as primers
54. The SPT3 gene is required for normal transcription of Ty elements in S. cerevisiae
55. Human immunodeficiency virus type 1 integrase protein promotes reverse transcription through specific interactions with the nucleoprotein reverse transcription complex
56. Functional RT and IN incorporated into HIV-1 particles independently of the Gag/Pol precursor protein