Functional RT and IN incorporated into HIV-1 particles independently of the Gag/Pol precursor protein

EMBO Journal

Volumn 16, Issue 16, 1997, Pages 5113-5122

  Wu, Xiaoyun ^a   Liu, Hongmei ^a   Xiao, Hongling ^a   Conway, Joan A ^a   Hunter, Eric ^b   Kappes, John C ^a,b,c  

^a Center for Palliative Care   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^c BIRMINGHAM VA MEDICAL CENTER   (United States)

Author keywords

Assembly; HIV 1; Integrase; Reverse transcriptase; Vpr

Indexed keywords

GAG PROTEIN; HYBRID PROTEIN; INTEGRASE; POL PROTEIN; PROTEIN PRECURSOR; RNA DIRECTED DNA POLYMERASE;

ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RETROVIRUS; VIRION; VIRUS ASSEMBLY; VIRUS INFECTION; VIRUS PARTICLE; VIRUS REPLICATION;

BLOTTING, WESTERN; CELL LINE; GENE EXPRESSION REGULATION, VIRAL; GENE PRODUCTS, GAG; GENE PRODUCTS, VPR; GENETIC COMPLEMENTATION TEST; HELA CELLS; HIV INTEGRASE; HIV-1; HIV-1 REVERSE TRANSCRIPTASE; HUMANS; MUTATION; PROTEIN PRECURSORS; RECOMBINANT FUSION PROTEINS; RETROVIRIDAE PROTEINS; TRANSFECTION; VIRAL REGULATORY PROTEINS; VIRUS ASSEMBLY;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; UNIDENTIFIED RETROVIRUS;

EID: 0030851908     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.16.5113     Document Type: Article

References (33)

1. Ansari-Lari, M.A. and Gibbs, R.A. (1996) Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection. J. Virol., 70, 3870-3875.
2. Ansari-Lari, M.A., Donehower, L.A. and Gibbs, R.A. (1995) Analysis of human immunodeficiency virus type 1 integrase mutants. Virology, 211, 332-335.
3. Bukovsky, A. and Göttlinger, H. (1996) Lack of integrase can markedly affect human immunodeficiency virus type 1 particle production in the presence of an active viral protease. J. Virol., 70, 6820-6825.
4. Cannon, P.M., Wilson, W., Byles, E., Kingsman, S.M. and Kingsman, A.J. (1994) Human immunodeficiency virus type 1 integrase: Effect on viral replication of mutations at highly conserved residues. J. Virol., 68, 4768-4775.
5. Coffin, J.M. (1990) Retroviridae and their replication. In Fields, B.N. and Knipe, D.M (eds), Fields Virology 2nd edn. Raven Press, NY, Vol. 2, pp. 1437-1500.
6. Dickson, C., Eisenmam, R., Fan, H., Hunter, E. and Teich, N. (1982) Protein biosynthesis and assembly. In Weiss, R., Teich, N., Varmus, H. and Coffin, J. (eds). RNA Tumor Viruses. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, Vol. 1, pp. 513-648.
7. Dubay, J.W., Roberts, S.J., Hahn, B.H. and Hunter, E. (1992) Truncation of the human immunodeficiency virus type 1 transmembrane glycoprotein cytoplasmic domain blocks virus infectivity. J. Virol., 66, 6616-6625.
8. Engelman, A. and Craigie, R. (1992) Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro. J. Virol., 66, 6361-6369.
9. Engelman, A., Englund, G., Orenstein, J.M., Martin, M.A. and Craigie, R. (1995) Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication. J. Virol., 69, 2729-2736.
10. Gallay, P., Swingler, S., Song, J., Bushman, F. and Trono, D. (1995) HIV nuclear import is governed by the phosphotyrosine-mediated binding of matrix to the core domain of integrase. Cell, 83, 569-576.
11. Ghosh, S.K., Fultz, P.N., Keddie, E., Saag, M.S., Sharp, P.M., Hahn, B.H. and Shaw, G.M. (1993) A molecular clone of HIV-1 tropic and cytopathic for human and chimpanzee lymphocytes. Virology, 194, 858-864.
12. Goff, S.P. (1990) Retroviral reverse transcriptase: synthesis, structure and function. J. Acquired Immune Defic. Syndr., 3, 817-831.
13. Jackson, J.B., Kwok, S.Y., Sninsky, J.J., Hopsicker, J.S., Sannerud, K.J., Rhame, F.S., Henry, K., Simpson, M. and Halford, H.H.J. (1990) Human immunodeficiency virus type 1 detected in all seropositive symptomatic and asymptomatic individuals. J. Clin. Microbiol., 28, 16-19.
14. Katz, R.A. and Skalka, A.M. (1994) The retroviral enzymes. In Richardson, C.S. (ed.), Annual Reviews Biochemistry. Annual Reviews, Inc., Palo Alto, CA, Vol. 63, pp. 133-173.
15. Kimpton, J. and Emerman, M. (1992) Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated β-galactosidase gene. J. Virol., 66, 2232-2239.
16. Kohl, N.E., Emini, E.A., Schlief, W.A., Davis, L.J., Heimbach, J.C., Dixon, R.A.F., Scolnick, E.M. and Sigal, I.S. (1988) Active human immunodeficiency virus protease is required for viral infectivity. Proc. Natl Acad. Sci. USA, 85, 4686-4690.
17. Leavitt, A.D., Robles, G., Alesandro, N. and Varmus, H.E. (1996) Human immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to integrate viral DNA efficiently during infection. J. Virol., 70, 721-728.
18. Liu, H., Wu, X., Newman, M., Shaw, G.M., Hahn, B.H. and Kappes, J.C. (1995) The Vif protein of human and simian immunodeficiency viruses is packaged into virions and associates with viral core structures. J. Virol., 69, 7630-7638.
19. Liu, H., Wu, X., Xiao, H., Conway, J.A. and Kappes, J.C. (1997) Incorporation of functional human immunodeficiency virus type I integrase into virions independent of the Gag/Pol precursor protein. J. Virol., 71, in press.
20. Luban, J., Lee, C. and Goff, S.P. (1993) Effect of linker insertion mutations in the human immunodeficiency virus type 1 gag gene on activation of viral protease expressed in bacteria. J. Virol., 67, 3630-3634.
21. Lys into human immunodeficiency virus type 1 particles. J. Virol., 68, 2065-2072.
22. Marquet, R., Isel, C., Ehresmann, C. and Ehresmann, B. (1995) tRNAs as primer of reverse transcriptases. Biochimie, 77, 113-124.
23. Masuda, T., Planelles, V. Krogstad, P. and Chen, I.S.Y. (1995) Genetic analysis of human immunodeficiency virus type 1 integrase and the U3 att site: unusual phenotype of mutants in the zinc finger-like domain. J. Virol., 69, 6687-6696.
24. Reicin, A.S., Paik, S., Berkowitz, R.D., Luban, J., Lowy, I. and Goff, S. (1995) Linker insertion mutations in the human immunodeficiency virus type 1 gag gene: Effects on virion particle assembly, release, and infectivity. J. Virol., 69, 642-650.
25. Shin, C.-G., Taddeo, B., Haseltine, W.A. and Farnet, C.M. (1994) Genetic Analysis of the Human Immunodeficiency Virus Type 1 Integrase Protein. J. Virol., 68, 1633-1642.
26. Trono, D. (1992) Partial reverse transcripts in virions from human immunodeficiency and murine leukemia viruses. J. Virol., 66, 4893-4900.
27. Varmus, H. and Swanstrom, R. (1982) Replication of retroviruses. In Weiss, R., Teich, N., Varmus, H. and Coffin, J. (eds), RNA Tumor Viruses. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 369-512.
28. Wiskerchen, M. and Muesing, M.A. (1995) Human immunodeficiency virus type 1 integrase: Effects of mutations on viral ability to integrate, direct viral gene expression from unintegrated viral DNA templates, and sustained viral propagation in primary cells. J. Virol., 69, 376-386.
29. Wu, X., Conway, J.A., Kim, J. and Kappes, J.C. (1994) Localization of the Vpx packaging signal within the C terminus of the human immunodeficiency virus type-2 Gag precursor protein. J. Virol., 68, 6161-6169.
30. Wu, X., Liu, H., Xiao, H., Kim, J., Seshaiah, P., Natsoulis, G., Boeke, J.D., Hahn, B.H. and Kappes, J.C. (1995) Targeting foreign proteins to human immunodeficiency virus particles via fusion with Vpr and Vpx. J. Virol., 69, 3389-3398.
31. Wu, X., Liu, H., Xiao, H., Conway, J.A. and Kappes, J.C. (1996a) Inhibition of human and simian immunodeficiency virus protease function by targeting Vpx-protease-mutant fusion protein into viral particles. J. Virol., 70, 3378-3384.
32. Wu, X., Liu, H., Xiao, H. and Kappes, J.C. (1996b) Proteolytic activity of human immunodeficiency virus Vpr-and Vpx-protease fusion proteins. Virology, 219, 307-313.
33. Zybarth, G. and Carter, C. (1995) Domains upstream of the protease (PR) in human immunodeficiency virus type 1 gag-pol influence PR autoprocessing. J. Virol., 69, 3878-3884.