Generation of myo-inositol-auxotrophic Leishmania mexicana mutants by targeted replacement of the myo-inositol-1-phosphate synthase gene

Molecular and Biochemical Parasitology

Volumn 120, Issue 1, 2002, Pages 151-156

  Ilg, Thomas ^a,b  

^a MAX PLANCK INST FÜR BIOLOGIE   (Germany)

^b Intervet Innovation GmbH   (Germany)

Author keywords

Glycosylphosphatidylinositol; Leishmania; myo Inositol auxotrophy; myo Inositol 1 phosphate synthase; Targeted gene deletion; Virulence

Indexed keywords

GLYCOSYLPHOSPHATIDYLINOSITOL; MYOINOSITOL 1 PHOSPHATE SYNTHASE;

AMINO ACID SEQUENCE; ARTICLE; AUXOTROPHY; CONTROLLED STUDY; GENE DELETION; GENE MUTATION; GENE STRUCTURE; LEISHMANIA MEXICANA; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; VIRULENCE;

LEISHMANIA MEXICANA;

EID: 0036182624     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(01)00435-2     Document Type: Article

References (18)

1. 1-L-myo-inositol-1-phosphate synthase
2. Back in the water: The return of the inositol phosphates
3. The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes
4. Glycoconjugates in Leishmania infectivity
5. Proteophosphoglycans of Leishmania
6. Characterization of inositol lipids from Leishmania donovani promastigotes: Identification of an inositol sphingophospholipid
7. + symporter from Leishmania donovani
8. + symporter from Leishmania donovani
9. Four conserved cytoplasmic sequence motifs are important for transport function of the Leishmania inositol/H(+) symporter
10. Ser/Thr-rich repetitive motifs as targets for phosphoglycan modifications in Leishmania mexicana secreted acid phosphatase
11. Phosphoglycan repeat-deficient Leishmania mexicana parasites remain infectious to macrophages and mice
12. Molecular cloning and characterization of a novel repeat-containing Leishmania major gene, ppg1, that encodes a membrane-associated form of proteophosphoglycan with a putative glycosylphosphatidylinositol anchor
13. Lipophosphoglycan is not required for infection of macrophages or mice by Leishmania mexicana
14. Glycosylation defects and virulence phenotypes of Leishmania mexicana phosphomannomutase and dolicholphosphate-mannosesynthase gene deletion mutants
15. A. mitogen-activated protein (MAP) kinase homologue of Leishmania mexicana is essential for parasite survival in the infected host
16. Disruption of mannose activation in Leishmania mexicana: GDP-mannose pyrophosphorylase is required for virulence, but not for viability
17. Substrate depletion upregulates uptake of myo-inositol, glucose and adenosine in Leishmania
18. Inositol metabolism in Trypanosoma cruzi: Potential target for chemotherapy against Chagas' disease