Thermostable NADP+-dependent medium-chain alcohol dehydrogenase from Acinetobacter sp. Strain M-1: Purification and characterization and gene expression in Escherichia coli

Applied and Environmental Microbiology

Volumn 66, Issue 12, 2000, Pages 5231-5235

  Tani, A ^a   Sakai, Y ^a   Ishige, T ^a   Kato, N ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DEHYDROGENASE; ALCOHOL DERIVATIVE; ALDEHYDE DERIVATIVE; ALKYL GROUP; ANISALDEHYDE; BENZALDEHYDE; CINNAMALDEHYDE; HEXADECANE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; RECOMBINANT ENZYME; ZINC;

ACINETOBACTER; ALCOHOL METABOLISM; ARTICLE; BACTERIAL GENE; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GENE EXPRESSION; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; REDUCTION; SEQUENCE HOMOLOGY; THERMOSTABILITY;

ACINETOBACTER; ALCOHOL OXIDOREDUCTASES; ALDEHYDES; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; RESTRICTION MAPPING; SUBSTRATE SPECIFICITY; TEMPERATURE;

ACINETOBACTER; ACINETOBACTER SP.; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA;

EID: 0034469069     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.66.12.5231-5235.2000     Document Type: Article

References (12)

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
2. Long-chain alcohol and aldehyde dehydrogenase activities in Acinetobacter calcoaceticus strain HO1-N
3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
4. A procedure for the isolation of deoxyribonucleic acid from micro-organisms
5. Alkane hydroxylase from Acinetobacter sp. strain ADP1 is encoded by alkM and belongs to a new family of bacterial integral-membrane hydrocarbon hydroxylases
6. Isolation of mutants of Acinetobacter calcoaceticus deficient in wax ester synthesis and complementation of one mutation with a gene encoding a fatty acyl coenzyme A reductase
7. A non-conventional dissimilation pathway for long chain n-alkanes in Acinetobacter sp. M-1 that starts with a dioxygenase reaction
8. Alcohol dehydrogenases in Acinetobacter sp. strain HO1-N: Role in hexadecane and hexadecanol metabolism
9. Detection of specific sequences among DNA fragments separated by gel electrophoresis
10. Substrate specificity of the alkane hydroxylase system of Pseudomonas oleovorans GPo1
11. Constitutive NADP-dependent alcohol dehydrogenase of Acinetobacter sp. strain HO1-N
12. NADP-dependent alcohol dehydrogenases in bacteria and yeast: Purification and partial characterization of the enzymes from Acinetobacter calcoaceticus and Saccharomyces cerevisiae