Expression of a functional NAD-reducing [NiFe] hydrogenase from the gram-positive Rhodococcus opacus in the gram-negative Ralstonia eutropha

Archives of Microbiology

Volumn 177, Issue 2, 2002, Pages 159-166

  Porthun, Antje ^a   Bernhard, Michael ^a   Friedrich, Bärbel ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

Bidirectional NAD reducing NiFe hydrogenase; Heterologous gene expression; Ralstonia eutropha; Rhodococcus opacus

Indexed keywords

BENZYLVIOLOGEN; HYDROGEN; HYDROGENASE; MAGNESIUM SULFATE; NICKEL CHLORIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE REDUCING [NICKEL IRON]HYDROGENASE; OXIDOREDUCTASE; PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CONTROLLED STUDY; CYANOBACTERIUM; ENZYME ACTIVITY; GRAM NEGATIVE BACTERIUM; HETEROLOGOUS EXPRESSION; HOST; HOXFUYH GENE; HOXW GENE; HYPA2B2F2 GENE; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECOMBINANT PLASMID; RHODOCOCCUS; RHODOCOCCUS OPACUS; WAUTERSIA EUTROPHA;

CUPRIAVIDUS NECATOR; HYDROGENASE; NAD; OXIDATION-REDUCTION; OXIDOREDUCTASES; RECOMBINANT PROTEINS; RHODOCOCCUS;

BACTERIA (MICROORGANISMS); CYANOBACTERIA; INSERTION SEQUENCES; NEGIBACTERIA; POSIBACTERIA; PROTEOBACTERIA; RALSTONIA; RHODOCOCCUS; RHODOCOCCUS OPACUS; UNCULTURED ACTINOMYCETE; WAUTERSIA EUTROPHA;

EID: 0036166884     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00203-001-0371-5     Document Type: Article

References (36)

1. The structure and mechanism of iron-hydrogenases
2. Learning from hydrogenases: Location of a proton pump and of a second FMN in bovine NADH-ubiquinone oxidoreductase (complex I)
3. The Alcaligenes eutrophus membrane-bound hydrogenase gene locus encodes functions involved in maturation and electron transport coupling
4. Interplay between the specific chaperon-like proteins HybG and HypC in maturation of hydrogenases 1, 2, and 3 from E. coli
5. XL1-blue: A high efficiency plasmid transforming recA- Escherichia coli strain with betagalactosidase selection
6. Maturation of the [NiFe] hydrogenases
7. hyp gene products in Alcaligenes eutrophus are part of a hydrogenase-maturation system
8. Molecular biology of hydrogen utilization in aerobic chemolithothrophs
9. Nickel requirement for hydrogenase formation in Alcaligenes eutrophus
10. Formation of enzymes of autotrophic metabolism during heterotrophic growth of Alcaligenes eutrophus
11. Nickel in the catalytically active hydrogenase of Alcaligenes eutrophus
12. Genes encoding the NAD-reducing hydrogenase of Rhodococcus opacus MR11
13. Location, catalytic activity, and subunit composition of NAD-reducing hydrogenases of some Alcaligenes strains and Rhodococcus opacus MR22
14. Towards optimization of cyanobacteria as biotechnologically relevant producers of molecular hydrogen, a clean and renewable energy source
15. 2-activating site of the cytoplasmic NAD-reducing hydrogenase from Ralstonia eutropha
16. Structural aspects of the soluble NAD-dependent hydrogenase isolated from Alcaligenes eutrophus H16 and from Nocardia opaca 1b
17. 2 sensor of Ralstonia eutropha is a member of the sub-class of regulatory [NiFe] hydrogenases
18. Protein measurement with the Folin phenol reagent
19. Dissection of the maturation reactions of the [NiFe] hydrogenase 3 from Escherichia coli taking place after nickel incorporation
20. Nickel incorporation into hydrogenases
21. Subforms and in vitro reconstitution of the NAD-reducing hydrogenase of Alcaligenes eutrophus
22. A novel FeS cluster in Fe-only hydrogenases
23. Carbamoylphosphate requirement for synthesis of the active center of [NiFe]-hydrogenases
24. Relationship between mitochondrial NADH-ubiquinone reductase and a bacterial NAD-reducing hydrogenase
25. Heterologous expression of the Desulfovibrio gigas [NiFe] hydrogenase in Desulfovibrio fructosovorans MR400
26. Ein submersverfahren zur kultur wasserstoff-oxidierender bakterien: Wachstumsphysiologische untersuchungen
27. Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H16
28. Effect of nickel on activity and subunit composition of purified hydrogenase from Nocardia opaca 1b
29. Transcriptional regulation of Alcaligenes eutrophus hydrogenase genes
30. A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in Gram-negative bacteria
31. Carboxyl-terminal processing of the cytoplasmatic NAD-reducing hydrogenase of the Alcaligenes eutrophus requires the hoxW gene product
32. Electrophoretic transfer of protein from polyacrylamide to nitrocellulose sheets: Procedure and some applications
33. Cloning and nucleotide sequences of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16
34. Crystal structure of nickel-iron hydrogenase from Desulfovibrio gigas
35. Duplication of hyp genes involved in maturation of [NiFe] hydrogenases in Alcaligenes eutrophus H16