Subforms and in vitro reconstitution of the NAD-reducing hydrogenase of Alcaligenes eutrophus

Journal of Bacteriology

Volumn 180, Issue 5, 1998, Pages 1023-1029

  Massanz, Christian ^a   Schmidt, Silke ^a   Friedrich, Bärbel ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; ISOENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE;

ARTICLE; BIOGENESIS; CATALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STRUCTURE; GENE DELETION; GENE EXPRESSION; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; STRUCTURAL GENE; WAUTERSIA EUTROPHA;

ALCALIGENES; BACTERIA (MICROORGANISMS); NEGIBACTERIA; WAUTERSIA EUTROPHA;

EID: 0031886480     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.5.1023-1029.1998     Document Type: Article

References (50)

1. Adams, M. W. W. 1990. The structure and mechanism of iron hydrogenases. Biochim. Biophys. Acta 1020:115-145.
2. Aggag, M., and H. G. Schlegel. 1974. Studies on a gram-positive hydrogen bacterium, Nocardia opaca strain 1b. III. Purification, stability and some properties of the soluble hydrogen dehydrogenase. Arch. Mikrobiol. 100:25-39.
3. Albracht, S. P. J. 1993. Intimate relationships of the large and the small subunits of all nickel hydrogenases with two nuclear-encoded subunits of mitochondrial NADH:ubiquinone oxidoreductase. Biochim. Biophys. Acta 1144:221-224.
4. Albracht, S. P. J. 1994. Nickel hydrogenases: in search of the active site. Biochim. Biophys. Acta 1188:167-204.
5. Appel, J., and R. Schulz. 1996. Sequence analysis of an operon of a NAD(P)-reducing nickel hydrogenase from the cyanobacterium Synechocystis sp. PCC6803 gives additional evidence for direct coupling of the enzyme to NAD(P)H dehydrogenase (complex I). Biochim. Biophys. Acta 1298:141-147.
6. Bernhard, M., B. Benelli, A. Hochkoeppler, D. Zanoni, and B. Friedrich. 1997. The membrane-bound hydrogenase (MBH) of Alcaligenes eutrophus H16: functional and structural role of the cytochrome b subunit. Eur. J. Biochem. 248:179-186.
7. Bernhard, M., E. Schwartz, J. Rietdorf, and B. Friedrich. 1996. The Alcaligenes eutrophus membrane-bound hydrogenase gene locus encodes functions involved in maturation and electron transport coupling. J. Bacteriol. 178: 4522-4529.
8. Boison. G., O. Schmitz, L. Mikheeva, S. Shestakov, and H. Bothe. 1996. Cloning, molecular analysis and insertional mutagenesis of the bidirectional hydrogenase genes from the cyanobacterium Anacystis nidulans. FEBS Lett. 394:153-158.
9. Cammack, R., V. M. Fernandez, and E. C. Hatchikian. 1994. Nickel-iron hydrogenase. Methods Enzymol. 234:43-68.
10. Dernedde, J., T. Eitinger, N. Patenge, and B. Friedrich. 1996. hyp gene products in Alcaligenes eutrophus are part of a hydrogenase-maturation system. Eur. J. Biochem. 235:351-358.
11. Eismann, K., K. Mlejnek, D. Zipprich, M. Hoppert, H. Gerberding, and F. Mayer. 1995. Antigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm. J. Bacteriol. 177:6309-6312.
12. Erkens, A., K. Schneider, and A. Müller. 1996. The NAD-linked soluble hydrogenase from Alcaligenes eutrophus H16: detection and characterisation of EPR signals deriving from nickel and flavin. J. Biol. Inorg. Chem. 1:99-110.
13. Friedrich, B., M. Bernhard, J. Dernedde, T. Eitinger, O. Lenz, C. Massanz, and E. Schwartz. 1996. Hydrogen oxidation by Alcaligenes, p. 110-117. In M. E. Lindstrom and F. R. Tabita (ed.), Microbial growth on C1 compounds. Kluwer Academic Publishers, Dordrecht, The Netherlands.
14. Friedrich, B., E. Heine, A. Finck, and C. G. Friedrich. 1981. Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus. J. Bacteriol. 145:1144-1149.
15. Friedrich, B., and E. Schwartz. 1993. Molecular biology of hydrogen utilization in aerobic chemolithotrophs. Annu. Rev. Microbiol. 47:351-383.
16. Friedrich, C. G., K. Schneider, and B. Friedrich. 1982. Nickel in the catalytically active hydrogenase of Alcaligenes eutrophus. J. Bacteriol. 152:42-48.
17. Grzesnik, C., M. Lübbers, M. Reh, and H. G. Schlegel. 1997. Genes encoding the NAD-reducing hydrogenase of Rhodococcus opacus MR11. Microbiology 24:1271-1286.
18. Grzesnik, C., K. Roß, K. Schneider, M. Reh, and H. G. Schlegel. 1997. Location, catalytic activity, and subunit composition of NAD-reducing hydrogenases of some Alcaligenes strains and Rhodococcus opacus MR22. Arch. Microbiol. 167:172-176.
19. Happe, R. P., W. Roseboom, K. A. Baglay, A. J. Pierik, and S. P. J. Albracht. 1997. Biological activation of hydrogen. Nature 385:126.
20. Hornhardt, S., K. Schneider, and H. G. Schlegel. 1986. Characterisation of a native subunit of the NAD-linked hydrogenase isolated from a mutant of Alcaligenes eutrophus H16. Biochimie 68:15-24.
21. Johannssen, W., H. Gerberding, M. Rohde, C. Zaborosch, and F. Mayer. 1991. Structural aspects of the soluble NAD-dependent hydrogenase isolated from Alcaligenes eutrophus H16 and from Nocardia opaca 1b. Arch. Microbiol. 155:303-308.
22. Knauf, V. C., and E. W. Nester. 1982. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti plasmid. Plasmid 8:45-54.
23. Kortlüke, C., K. Horstmann, E. Schwartz, M. Rohde, R. Binsack, and B. Friedrich. 1992. A gene complex coding for the membrane-bound hydrogenase of Alcaligenes eutrophus H16. J. Bacteriol. 174:6277-6289.
24. Lenz, O., E. Schwartz, J. Dernedde, M. Eitinger, and B. Friedrich. 1994. The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation. J. Bacteriol. 176:4385-4393.
25. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
26. Maier, T., and A. Böck. 1996. Nickel incorporation into hydrogenases, p. 173-192. In R. P. Hausinger, G. L. Eichhorn, and L. G. Marzilli (ed.), Advances in inorganic biochemistry, vol. 11. Mechanisms of metallocenter assembly. VCH Publishers Inc., New York, N.Y.
27. Malki, S., I. Saimmaime, G. De Luca, M. Rousset, Z. Dermoun, and J.-P. Belaich. 1995. Characterization of an operon encoding an NADP-reducing hydrogenase in Desulfovibrio fructosovorans. J. Bacteriol. 177:2628-2636.
28. 2-activating subunit, HoxH, directs maturation of the NAD-reducing hydrogenase in Alcaligenes eutrophus. Eur. J. Biochem. 245:441-448.
29. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
30. Oh, J.-I., and B. Bowien. 1997. Characterisation of the fds operon encoding the soluble formate dehydrogenase in Ralstonia eutropha. Biospectrum 1997: 127.
31. Perbal, B. 1988. A practical guide to molecular cloning, 2nd ed., p. 278-296. John Wiley & Sons, Inc., New York, N.Y.
32. Pilkington, S. J., J. M. Skehel, R. B. Gennis, and J. E. Walker. 1991. Relationship between mitochondrial NADH-ubiquinone reductase and a bacterial NAD-reducing hydrogenase. Biochemistry 30:2166-2175.
33. Przybyla, A. E., J. Robbins, N. Menon, and H. D. J. Peck, Jr. 1992. Structure/ function relationships among the nickel-containing hydrogenases. FEMS Microbiol. Rev. 88:109-136.
34. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
35. Sanger, F., S. Micklen, and A. R. Coulsen. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
36. 2 oxidation. J. Bacteriol. 175:3414-3421.
37. Schink, B., and H. G. Schlegel. 1979. The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification and biochemical properties. Biochem. Biophys. Acta 567:315-324.
38. Schlesier, M., and B. Friedrich. 1982. Effect of molecular hydrogen on histidine utilisation by Alcaligenes eutrophus. Arch. Mikrobiol. 132:260-265.
39. Schmitz, O., G. Boison, R. Hilscher, B. Hundeshagen, W. Zimmer, F. Lottspeich, and H. Bothe. 1995. Molecular biological analysis of a bidirectional hydrogenase from cyanobacteria. Eur. J. Biochem. 233:266-276.
40. Schneider, K., R. Cammack, and H. G. Schlegel. 1984. Content and localization of FMN, Fe-S clusters and nickel in the NAD-linked hydrogenase of Nocardia opaca 1b. Eur. J. Biochem. 142:75-84.
41. Schneider, K., and H. G. Schlegel. 1976. Purification and properties of the soluble hydrogenase from Alcaligenes eutrophus H16. Biochim. Biophys. Acta 452:66-80.
42. Schneider, K., H. G. Schlegel, and K. Jochim. 1984. Effect of nickel on activity and subunit composition of purified hydrogenase from Nocardia opaca 1b. Eur. J. Biochem. 138:533-541.
43. Simon, R., U. Priefer, and A. Pühler. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Bio/Technology 1:717-743.
44. Thiemermann, S., J. Dernedde, M. Bernhard, W. Schroeder, C. Massanz, and B. Friedrich. 1996. Carboxyl-terminal processing of the cytoplasmic NAD-reducing hydrogenase of Alcaligenes eutrophus requires the hoxW gene product. J. Bacteriol. 178:2368-2374.
45. Towbin, H., T. Staehlin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4357.
46. Tran-Betcke, A., U. Warnecke, C. Böcker, C. Zaborosch, and B. Friedrich. 1990. Cloning and nucleotide sequence of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16. J. Bacteriol. 172: 2920-2929.
47. Volbeda, A., M.-H. Charon, C. Piras, E. C. Hatchikian, M. Frey, and J. C. Fontecilla-Camps. 1995. Crystal structure of the nickel-iron hydrogenase from Desulfovibrio gigas. Nature 373:580-587.
48. Volbeda, A., E. Garcin, C. Piras, A. L. de Lacey, V. M. Fernandez, E. C. Hatchikian, M. Frey, and J. C. Fontecilla-Camps. 1996. Structure of the [NiFe] hydrogenase active site: evidence for biologically uncommon Fe ligands. J. Am. Chem. Soc. 118:12989-12996.
49. Weidner, U., S. Geier, A. Ptock, T. Friedrich, H. Leif, and H. Weiss. 1993. The gene locus of the proton-translocating NADH:ubiquinone oxidoreductase in Escherichia coli. J. Mol. Biol. 233:109-122.
50. Zaborosch, C., M. Köster, E. Bill, K. Schneider, H. G. Schlegel, and A. X. Trautwein. 1995. EPR and Mössbauer spectroscopic studies on the tetrameric, NAD-linked hydrogenase of Nocardia opaca 1b and its two dimers. 1. The βδ dimer - a prototype of a simple hydrogenase. BioMetals 8:149-162.