Marker-fusion PCR for one-step mutagenesis of essential genes in yeast

Yeast

Volumn 19, Issue 2, 2002, Pages 141-149

  Kitazono, Ana A ^a   Tobe, Brian T D ^a   Kalton, Helen ^a   Diamant, Noam ^a   Kron, Stephen J ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

Cdc28; Fusion PCR; Gene replacement; Integration; Mutagenesis; Screening

Indexed keywords

CELL CYCLE PROTEIN; OLIGONUCLEOTIDE;

ALLELE; ARTICLE; CONTROLLED STUDY; ESSENTIAL GENE; FUNGUS MUTATION; GENE LIBRARY; GENE LOCUS; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; GENOME; MARKER GENE; MOLECULAR CLONING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RANDOMIZATION; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STRAIN DIFFERENCE;

GENES, FUNGAL; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; YEASTS;

SACCHAROMYCETALES;

EID: 0036158779     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.806     Document Type: Article

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