A scintillation proximity assay amenable for screening and characterization of DNA gyrase B subunit inhibitors

Analytical Biochemistry

Volumn 300, Issue 1, 2002, Pages 34-39

  Gevi, Monica ^a   Domenici, Enrico ^a  

^a GSK   (Italy)

Author keywords

ATPase; DNA gyrase; Ligand binding; Novobiocin; Scintillation proximity assay

Indexed keywords

BINDING ENERGY; COENZYMES; DIMETHYL SULFOXIDE; DISSOCIATION; SCINTILLATION;

96-WELL MICROTITER PLATE; ATPASE; DNA GYRASES; EQUILIBRIUM DISSOCIATION CONSTANT; HIGH-THROUGHPUT SCREENS; INHIBITORY CONCENTRATION; LIGAND BINDING; NOVOBIOCIN;

DNA;

ANTIINFECTIVE AGENT; COUMARIN; CYCLOTHIAZIDE; DNA TOPOISOMERASE (ATP HYDROLYSING); GR 122222X; GYRASE INHIBITOR; NOVOBIOCIN;

ARTICLE; BINDING SITE; DNA SCREENING; DRUG PROTEIN BINDING; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; SCREENING TEST;

EID: 0036149670     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5406     Document Type: Article

References (18)

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14. An improved assay for nanomole amounts of inorganic phosphate
15. Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction
16. The nature of inhibition of DNA gyrase by the coumarins and the cyclothialidines revealed by X-ray crystallography