Agrobacterium-meâiãteó plant transformation: A scientifically intriguing story with significant applications

Transgenic Plant Research

Volumn , Issue , 2022, Pages 1-33

  Gheysen, Godelieve ^a,b   Angenon, Geert ^a   van Montagu, Marc ^a  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

Agrobacterium tumefaciens; Plant transformation; Regeneration; Selectable marker; T DNA; Virulence

Indexed keywords

EID: 85127630528     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (373)

1. Albano, M., Breitling, R. and Dubnau, D.A. (1989). Nucleotide sequence and genetic organization of the Bacillus subtilis comG operon. Journal of Bacteriology, 171, 5386-5404.
2. Aldemita, R.R. and Hodges, T.K. (1996). Agrobacterium tumefaciens-meáiãteá transformation of japónica and indica rice varieties. Planta, 199, 612-617.
3. Allen, G.C., Hall, G.E. Jr, Childs, L.C., Weissinger, A.K., Spiker, S. and Thompson, W.F. (1993). Scaffold attachment regions increase reporter gene expression in stably transformed plant cells. The Plant Cell, 5, 603-613.
4. Allen, G.C., Hall, G. Jr, Michałowski, S., Newman, W., Spiker, S., Weissinger, A.K. and Thompson, W.F. (1996). High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco. The Plant Cell, 8, 899-913.
5. Alt-Moerbe, J., Neddermann, P., von Lintig, J., Weiler, E.W. and Schröder, J. (1988). Temperature-sensitive step in Ti plasmid vir-region induction and correlation with cytokinin secretion by Agrobacteria. Molecular and General Genetics, 213, 1-8.
6. Alt-Moerbe, J., Kühlmann, H. and Schröder, J. (1989). Differences in induction of Ti plasmid virulence genes virG and virD, and continued control of virD expression by four external factors. Molecular Plant-Microbe Interactions, 2, 301-308.
7. Ambros, P.F., Matzke, A.J.M. and Matzke, M.A. (1986). Localization of Agrobacterium rhizogenes T-DNA in plant chromosomes by in situ hybridization. EMBO Journal, 5, 2073-2077.
8. Ankenbauer, R.G. and Nester, E.W. (1990). Sugar-mediated induction of Agrobacterium tumefaciens virulence genes: structural specificity and activities of monosaccharides. Journal of Bacteriology, 172, 6442-6446.
9. Aragão, F.J.L., Barros, L.M.G., Brasileiro, A.C.M., Ribeiro, S.G., Smith, F.D., Sanford, J.C., Faria, J.C. and Rech, E.L. (1996). Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment. Theoretical and Applied Genetics, 93 142-150.
10. Ashby, A.M., Watson, M.D. and Shaw, CH. (1987). A Ti plasmid determined function is responsible for Chemotaxis of A. tumefaciens towards the plant wound compound acetosyringone. FEMS Microbiology Läters, 41, 189-192.
11. 1 toward vir-inducing phenolic compounds and soluble factors from monocotyle-donous and dicotyledonous plants. Journal of Bacteriology, 170, 4181-4187.
12. Banta, L.M., Joerger, R.D., Howitz, V.R., Campbell, A.M. and Binns, A.N. (1994). Glu-255 outside the predicted ChvE binding site in Vir A is crucial for sugar enhancement of acetosyringone perception by Agrobacterium tumefaciens. Journal of Bacteriology, 176, 3242-3249.
13. Baron, C. and Zambryski, P.C. (1996). Plant transformation: A pilus in Agrobacterium T-DNA transfer. Current Biology, 6, 1567-1569.
14. Baron, C, Thorstenson, Y.R. and Zambryski, P.C. (1997a). The lipoprotein VirB7 interacts with VirB9 in the membranes of Agrobacterium tumefaciens. Journal of Bacteriology, 179, 1211-1218.
15. Baron, C, Llosa, M., Zhou, S. and Zambryski, P.C. (1997b). VirBl, a component of the T-complex transfer machinery of Agrobacterium tumefaciens, is processed to a C-terminal secreted product, VirBl. Journal of Bacteriology, 179, 1203-1210.
16. Bean, S.J., Gooding, P.S., Mullineaux, P.M. and Davies, D.R. (1997). A simple system for pea transformation. Plant Cell Reports, 16, 513-519.
17. Beaupré, CE., Bohne, J., Dale, E.M. and Binns, A.N. (1997). Interactions between VirB9 and VirBlO membrane proteins involved in movement of DNA from Agrobacterium tumefaciens into plant cells. Journal of Bacteriology, 179, 78-89.
18. Bechtold, N. and Bouchez, D. (1995). In planta Agrobac-terium-meáiàteá transformation of adult Arabidopsis thaliana plants by vacuum infiltration. In Gene Transfer to Plants, (Springer Lab Manual), edited by I. Potrykus and G. Spangenberg, pp. 19-23. Berlin: Springer-Verlag.
19. Bechtold, N., Ellis, J. and Pelletier, G. (1993). In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. Comptes Rendus de l'Académie des Sciences, Série III, Sciences de la Vie, 316, 1194-1199.
20. Beijersbergen, A., den Dulk-Ras, A., Schilperoort, R.A. and Hooykaas, P.J.J. (1992). Conjugative transfer by the virulence system of Agrobacterium tumefaciens. Science, 256, 1324-1327.
21. Beijersbergen, A., Smith, S.J. and Hooykaas, P.J.J. (1994). Localization and topology of VirB proteins of Agrobacterium tumefaciens. Plasmid, 32, 212-218.
22. Bélanger, C, Loubens, L, Nester, E.W. and Dion, P. (1997). Variable efficiency of a Ti plasmid-encoded VirA protein in different agrobacterial hosts. Journal of Bacteriology, 179, 2305-2313.
23. Benediktsson, I., Spampinato, CP. and Schieder, O. (1995). Studies of the mechanism of transgene integration into plant protoplasts: improvement of the transformation rate. Euphytica, 85, 53-61.
24. Bidney, D., Scelonge, C, Martich, J., Burrus, M., Sims, L. and Huffman, G. (1992). Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens. Plant Molecular Biology, 18, 301-313.
25. Binns, A.N. and Thomashow, M.F. (1988). Cell biology of Agrobacterium infection and transformation of plants. Annual Review of Microbiology, 42, 575-606.
26. Binns, A.N., Beaupré, CE. and Dale, E.M. (1995). Inhibition of VirB-mediated transfer of diverse substrates from Agrobacterium tumefaciens by the IncQ plasmid RSF1010. Journal of Bacteriology, 177, 4890-4899.
27. Bouchez, D., Camilleri, C and Caboche, M. (1993). A binary vector based on Basta resistance for in planta transformation of Arabidopsis thaliana. Comptes Rendus de l'Académie des Sciences, Série III, Sciences de la Vie, 316, 1188-1193.
28. Brar, G.S., Cohen, B.A., Vick, CL. and Johnson, G.W. (1994). Recovery of transgenic peanut (Arachis hypo-gaea L.) plants from elite cultivars using ACCELL® technology. The Plant Journal, 5, 745-753.
29. Braun, A.C. and White, P.R. (1943). Bacteriological sterility of tissues derived from secondary crown gall tumors. Phytopathology, 33, 85-100.
30. Breyne, P., Gheysen, G, Jacobs, A., Van Montagu, M. and Depicker, A. (1992a). Effect of T-DNA configuration on transgene expression. Molecular and General Genetics, 235, 389-396.
31. Breyne, P., Van Montagu, M., Depicker, A. and Gheysen, G. (1992b). Characterization of a plant scaffold attachment region in a DNA fragment that normalizes transgene expression in tobacco. The Plant Cell, 4, 463-471.
32. Broer, I., Dröge-Laser, W., Barker, R.F., Neumann, K., Klipp, W. and Pühler, A. (1995). Identification of the Agrobacterium tumefaciens C58 T-DNA genes e and f and their impact on crown gall tumour formation. Plant Molecular Biology, 27, 41-57.
33. Buchanan-Wollaston, V., Passiatore, J.E. and Cannon, F. (1987). The mob and oriT mobilization functions of a bacterial plasmid promote its transfer to plants. Nature, 328, 172-175.
34. Buchanan-Wollaston, V., Snape, A. and Cannon, F. (1992). A plant selectable marker gene based on the detoxification of the herbicide dalapon. Plant Cell Reports, 11, 627-631.
35. Bundock, P. and Hooykaas, P.J.J. (1996). Integration of Agrobacterium tumefaciens T-DNA in the Saccharomyces cerevisiae genome by illegitimate recombination. Proceedings of the National Academy of Sciences USA, 93, 15272-15275.
36. Bundock, P., den Dulk-Ras, A., Beijersbergen, A. and Hooykaas, P.J.J. (1995). Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. The EMBO Journal, 14, 3206-3214.
37. Burrus, M., Molinier, J., Himber, C, Hunold, R, Bronner, R, Rousselin, P. and Hahne, G. (1996). Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.) shoot apices: transformation patterns. Molecular Breeding, 2, 329-338.
38. Cangelosi, G.A., Hung, L., Puvanesarajah, V., Stacey, G, Ozga, D.A., Leigh, J.A. and Nester, E.W. (1987). Common loci for Agrobacterium tumefaciens and Rhizobium meliloti exopolysaccharide synthesis and their roles in plant interaction. Journal of Bacteriology, 169, 2086-2091.
39. Cangelosi, G.A., Martinetti, G, Leigh, J.A., Lee, CC, Theines, C. and Nester, E.W. (1989). Role of Agrobacterium tumefaciens ChvA protein in export of β-1,2-glucan. Journal of Bacteriology, 171, 1609-1615.
40. Cangelosi, G.A., Ankenbauer, R.G. and Nester, E.W. (1990). Sugars induce the Agrobacterium virulence genes through a periplasmic binding protein and a transmembrane signal protein. Proceedings of the National Academy of Sciences USA, 87, 6708-6712.
41. Carrer, H., Staub, J.M. and Maliga, P. (1991). Gentamycin resistance in Nicotiana conferred by AAC(3)-I, a narrow substrate specificity acetyltransferase. Plant Molecular Biology, 17, 301-303.
42. Castle, L.A. and Morris, R.O. (1990). A method for early detection of T-DNA transfer. Plant Molecular Biology Reporter, 8, 28-39.
43. Castle, L.A., Errampalli, D., Atherton, T.L., Franzmann, L.H., Yoon, E.S. and Meinke, D.W. (1993). Genetic and molecular characterization of embryonic mutants identified following seed transformation in Arabidopsis. Molecular and General Genetics, 241, 504-514.
44. Chang, S.S., Park, S.K., Kim, B.C., Kang, B.J., Kim, D.U. and Nam, H.G. (1994). Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta. The Plant Journal, 5, 551-558.
45. Charles, T.C and Nester, E.W. (1993). A chromosomally encoded two-component sensory transduction system is required for virulence of Agrobacterium tumefaciens. Journal of Bacteriology, 175, 6614-6625.
46. Cheng, M., Jarret, R.L., Li, Z., Xing, A. and Demski, J.W. (1996). Production of fertile transgenic peanut (Arachis hypogaea L.) plants using Agrobacteńum tumefaciens. Plant Cell Reports, 15, 653-657.
47. Chilton, M.-D., Drummond, M.H., Merlo, D.J., Sciaky, D., Montoya, A.L., Gordon, M.P. and Nester, E.W. (1977). Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumori-genesis. Cell, 11, 263-271.
48. Christie, P.J., Ward, J.E., Winans, S.C. and Nester, E.W. (1988). The Agrobacteńum tumefaciens viril gene product is a single-stranded-DNA-binding protein that associates with T-DNA. Journal of Bacteriology, 170, 2659-2667.
49. Christie, P.J., Ward, J.E. Jr, Gordon, M.P. and Nester, E.W. (1989). A gene required for transfer of T-DNA to plants encodes an ATPase with autophosphorylating activity. Proceedings of the National Academy of Sciences USA, 86, 9677-9681.
50. 0 transgenic soybean plantlets using tissue-specific expression patterns. The Plant Journal, 2, 283-290.
51. Christou, P., Swain, W.F., Yang, N.-S. and McCabe, D.E. (1989). Inheritance and expression of foreign genes in transgenic soybean plants. Proceedings of the National Academy of Sciences USA, 86, 7500-7504.
52. Chyi, Y.-S., Jorgensen, R.A., Goldstein, D., Tanksley, S.D. and Loaiza-Figueroa, F. (1986). Locations and stability of Agrobacterium-mediated T-DNA insertions in the Lycopersicon genome. Molecular and General Genetics, 204, 64-69.
53. Citovsky, V., Wong, M.L. and Zambryski, P. (1989). Cooperative interaction of Agrobacteńum VirE2 protein with single-stranded DNA: implications for the T-DNA transfer process. Proceedings of the National Academy of Sciences USA, 86, 1193-1197.
54. Citovsky, V., Zupan, J., Warnick, D. and Zambryski, P. (1992). Nuclear localization of Agrobacterium VirE2 protein in plant cells. Science, 256, 1802-1805.
55. Citovsky, V., Warnick, D. and Zambryski, P. (1994). Nuclear import of Agrobacterium VirD2 and VirE2 proteins in maize and tobacco. Proceedings of the National Academy of Sciences USA, 91, 3210-3214.
56. Cluster, P.D., O'Dell, M., Metzlaff, M. and Flavell, R.B. (1996). Details of T-DNA structural organization from a transgenic Petunia population exhibiting co-suppression. Plant Molecular Biology, 32, 1197-1203.
57. Comai, L. and Kosuge, T. (1983). The genetics of indole-acetic acid production and virulence in Pseudomonas savastanoi. In Molecular Genetics of the Bacteria-Plant Interaction, edited by A. Pühler, pp. 362-372. Berlin: Springer-Verlag.
58. Cooley, M.B., DSouza, M.R. and Kado, CI. (1991). The virC and virD opérons of the Agrobacteńum Ti plasmid are regulated by the ros chromosomal gene: analysis of the cloned ros gene. Journal of Bacteriology, 173, 2608-2616.
59. D'Souza-Ault, M.R., Cooley, M.B. and Kado, C.I. (1993). Analysis of the Ros repressor of Agrobacterium virC and virD opérons: molecular intercommunication between plasmid and chromosomal genes. Journal of Bacteriology, 175, 3486-3490.
60. Davies, D.R, Hamilton, J. and Mullineaux, P. (1993). Transformation of peas. Plant Cell Reports, 12, 180-183.
61. de Framond, A.J., Barton, K.A. and Chilton, M.-D. (1983). Mini-Ti: a new vector strategy for plant genetic engineering. Bio/technology, 1, 262-269.
62. de Kathen, A. and Jacobsen, H.-J. (1995). Cell competence for Agrobacterium-mediated DNA transfer in Pisum sativum L. Transgenic Research, 4, 184-191.
63. De Block, M. and Debrouwer, D. (1991). Two T-DNA's co-transformed into Brassica napus by a double Agrobacterium tumefaciens infection are mainly integrated at the same locus. Theoretical and Applied Genetics, 82, 257-263.
64. De Block, M., Herrera-Estrella, L., Van Montagu, M., Schell, J. and Zambryski, P. (1984). Expression of foreign genes in regenerated plants and their progeny. EMBO Journal, 3, 1681-1689.
65. De Block, M., Botterman, J., Vandewiele, M., Dockx, J., Thoen, C, Gosselé, V., Mowa, R., Thompson, C, Van Montagu, M. and Leemans, J. (1987). Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO Journal, 6, 2513-2518.
66. De Buck, S., Jacobs, A., Van Montagu, M. and Depicker, A. (1998). Transformation and cotransformation frequencies of Arabidopsis root expiants and tobacco protoplasts. Molecular Plant-Microbe Interactions, in press.
67. De Cleene, M. and De Ley, J. (1976). The host range of crown-gall. Bot. Rev., 42, 389-466.
68. De Jaeger, G., Buys, E., Eeckhout, D., De Wilde, C, Jacobs, A., Kapila, J., Angenon, G., Van Montagu, M., Geräts, T. and Depicker, A. (1998). High level accumulation of single-chain variable fragments in the cytosol of transgenic Petunia hybrida. Planta, in press.
69. De Neve, M., De Buck, S., Jacobs, A., Van Montagu, M. and Depicker, A. (1997). T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from ligation of separate T-DNAs. The Plant Journal, 11, 15-29.
70. Deblaere, R., Bytebier, B., De Greve, H., Deboeck, F., Schell, J., Van Montagu, M. and Leemans, J. (1985). Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants. Nucleic Acids Research, 13, 4777-4788.
71. Deng, W., Pu, X.-a., Goodman, R.N., Gordon, M.P. and Nester, E.W. (1995). T-DNA genes responsible for inducing a necrotic response on grape vines. Molecular Plant-Microbe Interactions, 8, 538-548.
72. Denis, M., Renard, M. and Krebbers, E. (1995). Isolation of homozygous transgenic Brassica napus lines carrying a seed-specific chimeric 2S albumin gene and determination of linkage relationships. Molecular Breeding, 1, 143-153.
73. Deroles, S.C. and Gardner, R.C. (1988). Analysis of the T-DNA structure in a large number of transgenic petunias generated by Agrobacterium-mediated transformation. Plant Molecular Biology, 11, 365-377.
74. Dessaux, Y., Petit, A. and Tempé, J. (1992). Opines in Agrobacterium biology. In Molecular Signals in Plant-Microbe Communications, edited by D.P.S. Verma, pp. 109-136. Boca Raton: CRC Press.
75. Di, R., Purcell, V., Collins, G.B. and Ghabrial, S.A. (1996). Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene. Plant Cell Reports, 15, 746-750.
76. Dillen, W., De Clercq, J., Goossens, A., Van Montagu, M. and Angenon, G. (1997a). Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray. Theoretical and Applied Genetics, 94, 151-158.
77. Dillen, W., De Clercq, J., Kapila, J., Zambre, M., Van Montagu, M. and Angenon, G. (1997b). The effect of temperature on Agrobacterium tumefaciens-mediated gene transfer to plants. The Plant Journal, 12, 1459-1463.
78. Dombek, P. and Ream, W. (1997). Functional domains of Agrobactenum tumefaciens single-stranded DNA-binding protein VirE2. Journal of Bacteriology, 179, 1165-1173.
79. Dong, J., Teng, W., Buchholz, W.G. and Hall, T.C. (1996). Agrobacterium-mediated transformation of Javanica rice. Molecular Breeding, 2, 267-276.
80. Dougherty, W.G. and Parks, T.D. (1995). Transgenes and gene suppression: telling us something new? Current Opinion in Cell Biology, 7, 399-405.
81. Douglas, C.J., Halperin, W. and Nester, E.W. (1982). Agrobacterium tumefaciens mutants affected in attachment to plant cells. Journal of Bacteriology, 152, 1265-1275.
82. Douglas, C, Halperin, W., Gordon, M. and Nester, E. (1985). Specific attachment of Agrobactenum tumefaciens to bamboo cells in suspension cultures. Journal of Bacteńology, 161, 764-766.
83. Dürrenberger, F., Crameri, A., Hohn, B. and Koukolíková-Nicola, Z. (1989). Covalently bound VirD2 protein of Agrobacterium tumefaciens protects the T-DNA from exonucleolytic degradation. Proceedings of the National Academy of Sciences USA, 86, 9154-9158.
84. Ebinuma, H., Sugita, K., Matsunaga, E. and Yamakado, M. (1997). Selection of marker-free transgenic plants using the isopentenyl transferase gene. Proceedings of the National Academy of Sciences USA, 94, 2117-2121.
85. Eichholtz, D.A., Rogers, S.G., Horsch, R.B., Klee, H.J., Hayford, M., Hoffmann, N.L., Bradford, S.B., Fink, C, Flick, J., O'Connell, K.M. and Fraley, R.T. (1987). Expression of mouse dihydrofolate reductase gene confers methotrexate resistance in transgenic petunia plants. Somatic Cell Molecular Genetics, 13, 67-76.
86. Elkind, Y., Nir, B. and Nadler-Hassar, T. (1995). Quantitative analysis of the transgene variability among primary tobacco transformants. Transgenic Research, 4, 30-38.
87. Ellis, J.G. and Murphy, P.J. (1981). Four new opines from crown gall tumours - their detection and properties. Molecular and General Genetics, 181, 36-43.
88. Elmayan, T. and Vaucheret, H. (1996). Expression of single copies of a strongly expressed 35S transgene can be silenced post-transcriptionally. The Plant Journal, 9, 787-797.
89. Endoh, H., Aoyama, T. and Oka, A. (1993). Transcription in vitro promoted by the Agrobacterium VirG protein. FEBS Letters, 334, 277-280.
90. Escudero, J., Neuhaus, G. and Hohn, B. (1995). Intracellular Agrobacterium can transfer DNA to the cell nucleus of the host plant. Proceedings of the National Academy of Sciences USA, 92, 230-234.
91. Feldmann, K.A. (1991). T-DNA insertion mutagenesis in Arabidopsis: mutational spectrum. The Plant Journal, 1, 71-82.
92. Feldmann, K.A. and Marks, M.D. (1987). Agrobacterium-mediated transformation of germinating seeds of Arabidopsis thaliana: a non-tissue culture approach. Molecular and General Genetics, 208, 1-9.
93. Fernandez, D., Dang, T.A.T., Spudich, G.M., Zhou, X.-R., Berger, B.R. and Christie, P.J. (1996a). The Agrobacterium tumefaciens virBl gene product, a proposed component of the T-complex transport apparatus, is a membrane-associated lipoprotein exposed at the periplasmic surface. Journal of Bacteriology, 178, 3156-3167.
94. Fernandez, D., Spudich, GM., Zhou, X.-R. and Christie, P.J. (1996b). The Agrobacterium tumefaciens VirB7 lipoprotein is required for stabilization of VirB proteins during assembly of the T-complex transport apparatus. Journal of Bacteriology, 178, 3168-3176.
95. Filichkin, S.A. and Gelvin, S.B. (1993). Formation of a putative relaxation intermediate during T-DNA processing directed by the Agrobacterium tumefaciens VirDl, D2 endonuclease. Molecular Microbiology, 8, 915-926.
96. Finnegan, J. and McElroy, D. (1994). Transgene inactiva-tion: plants fight back! Bio/technology, 12, 883-888.
97. Fla veil, R.B. (1994). Inactivation of gene expression in plants as a consequence of specific sequence duplication. Proceedings of the National Academy of Sciences USA, 91, 3490-3496.
98. Fobert, P.R., Labbé, H., Cosmopoulos, J., Gottlob-McHugh, S., Ouellet, T., Hattori, J., Sunohara, G, Iyer, V.N. and Miki, B.L. (1994). T-DNA tagging of a seed coat-specific cryptic promoter in tobacco. The Plant Journal, 6, 567-577.
99. Follin, A., Inzé, D., Budar, F., Genetello, C, Van Montagu, M. and Schell, J. (1985). Genetic evidence that the tryptophan 2-monooxygenase gene of Pseudomonas savastanoi is functionally equivalent to one of the T-DNA genes involved in plant tumour formation by Agrobacterium tumefaciens. Molecular and General Genetics, 201, 178-185.
100. Fraley, R.T., Horsch, R.B., Matzke, A., Chilton, M.-D., Chilton, W.S. and Sanders, P.R. (1984). In vitro transformation of petunia cells by an improved method of co-cultivation with A. tumefaciens strains. Plant Molecular Biology, 3, 371-378.
101. Fromm, M.E., Morrish, F., Armstrong, C, Williams, R., Thomas, J. and Klein, T.M. (1990). Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bioltechnology, 8, 833-839.
102. Fullner, K.J., Stephens, K.M. and Nester, E.W. (1994). An essential virulence protein of Agrobacterium tumefaciens, VirB4, requires an intact mononucleotide binding domain to function in transfer of T-DNA. Molecular and General Genetics, 245, 704-715.
103. Fullner, K.J., Lara, J.C. and Nester, E.W. (1996). Pilus assembly by Agrobacterium T-DNA transfer genes. Science, 273, 1107-1109.
104. Garbers, C, DeLong, A., Deruére, J., Bernasconi, P. and Söll, D. (1996). A mutation in protein phosphatase 2A regulatory subunit A affects auxin transport in Arabidopsis. The EMBO Journal, 15, 2115-2124.
105. Gaudin, V., Vrain, T. and Jouanin, L. (1994). Bacterial genes modifying hormonal balances in plants. Plant Physiology and Biochemistry, 32, 11-29.
106. Gelvin, S.B. and Liu, C.-N. (1994). Genetic manipulation of Agrobacterium tumefaciens strains to improve transformation of recalcitrant plant species. In Plant Molecular Biology Manual, 2nd edn, edited by S.B. Gelvin and R.A. Schilperoort, pp. B4:l-13. Dordrecht: Kluwer Academic Publishers.
107. Ghai, J. and Das, A. (1989). The virD Operon of Agrobacterium tumefaciens Ti plasmid encodes a DNA-relaxing enzyme. Proceedings of the National Academy of Sciences USA, 86, 3109-3113.
108. Gheysen, G, Dhaese, P., Van Montagu, M. and Schell, J. (1985). DNA flux across genetic barriers: the crown gall phenomenon. In Genetic Flux in Plants (Plant Gene Research, Vol. 2), edited by B. Hohn and E.S. Dennis, pp. 11-47. Wien: Springer Verlag.
109. Gheysen, G, Herman, L., Breyne, P., Gielen, J., Van Montagu, M. and Depicker, A. (1990). Cloning and analysis of truncated T-DNA inserts from Nicotiana tabacum. Gene, 94, 155-163.
110. Gheysen, G, Villarroel, R. and Van Montagu, M. (1991). Illegitimate recombination in plants: a model for T-DNA integration. Genes and Development, 5, 287-297.
111. Gietl, C, Koukolíková-Nicola, Z. and Hohn, B. (1987). Mobilization of T-DNA from Agrobacterium to plant cells involves a protein that binds single-stranded DNA. Proceedings of the National Academy of Sciences USA, 84, 9006-9010.
112. Goddijn, O.J.M., van der Duyn Schouten, P.M., Schilperoort, R.A. and Hoge, J.H.C. (1993). A chim-aeric tryptophan decarboxylase gene as a novel selectable marker in plant cells. Plant Molecular Biology, 22, 907-912.
113. Gosselé, V., van Aarssen, R. and Cornelissen, M. (1994). A 6' gentamicin acetyltransferase gene allows effective selection of tobacco transformants using kanamycin as a substrate. Plant Molecular Biology, 26, 2009-2012.
114. Grant, J.E., Cooper, P.A., McAra, A.E. and Frew, T.J. (1995). Transformation of peas (Pisum sativum L.) using immature cotyledons. Plant Cell Reports, 15, 254-258.
115. Gray, J., Wang, J. and Gelvin, S.B. (1992). Mutation of the miaA gene of Agrobacterium tumefaciens results in reduced vir gene expression. Journal of Bacteriology, 174, 1086-1098.
116. Grevelding, C, Fantes, V., Kemper, E., Schell, J. and Masterson, R. (1993). Single-copy T-DNA insertions in Arabidopsis are the predominant form of integration in root-derived transgenics, whereas multiple insertions are found in leaf discs. Plant Molecular Biology, 23, 847-860.
117. Guerineau, F., Brooks, L., Meadows, J., Lucy, A., Robinson, C. and Mullineaux, P. (1990). Sulfonamide resistance gene for plant transformation. Plant Molecular Biology, 15, 127-136.
118. Guivarc'h, A., Caissard, J.-C, Brown, S., Marie, D., Dewitte, W., Van Onckelen, H. and Chriqui, D. (1993). Localization of target cells and improvement of Agrobacterium-mediated transformation efficiency by direct acetosyringone pretreatment of carrot root discs. Protoplasma, 174, 10-18.
119. Hamilton, CM., Frary, A., Lewis, C. and Tanksley, S.D. (1996). Stable transfer of intact high molecular weight DNA into plant chromosomes. Proceedings of the National Academy of Sciences USA, 93, 9975-9979.
120. Han, D.C. and Winans, S.C. (1994). A mutation in the receiver domain of the Agrobacterium tumefaciens transcriptional regulator VirG increases its affinity for operator DNA. Molecular Microbiology, 12, 23-30.
121. Hänisch ten Cate, CH., Loonen, A.E.H.M., Ottaviani, M.P., Ennik, L., van Eldik, G. and Stiekema, W.J. (1990). Frequent spontaneous deletions of Ri T-DNA in Agrobacterium rhizogenes transformed potato roots and regenerated plants. Plant Molecular Biology, 14, 735-741.
122. Hansen, G. and Chilton, M.-D. (1996). "Agrolistic" transformation of plant cells: integration of T-strands generated in planta. Proceedings of the National Academy of Sciences USA, 93, 14978-14983.
123. Hansen, G, Das, A. and Chilton, M.-D. (1994). Constitutive expression of the virulence genes improves the efficiency of plant transformation by Agrobacterium. Proceedings of the National Academy of Sciences USA, 91, 7603-7607.
124. Hawes, M.C and Smith, L.Y. (1989). Requirement for Chemotaxis in pathogenicity of Agrobacterium tumefaciens on roots of soil-grown pea plants. Journal of Bacteriology, 171, 5668-5671.
125. Hayford, M.B., Medford, J.I., Hoffman, N.L., Rogers, S.G. and Klee, H.J. (1988). Development of a plant transformation selection system based on expression of genes encoding gentamicin acetyltransferases. Plant Physiology, 86, 1216-1222.
126. Herman, L., Jacobs, A., Van Montagu, M. and Depicker, A. (1990). Plant chromosome/marker gene fusion assay for study of normal and truncated T-DNA integration events. Molecular and General Genetics, 224, 248-256.
127. Herrera-Estrella, A., Chen, Z.-M., Van Montagu, M. and Wang, K. (1988). VirD proteins of Agrobacterium tumefaciens are required for the formation of a covalent DNA-protein complex at the 5' terminus of T-strand molecules. The EMBO Journal, 7, 4055-4062.
128. Herrera-Estrella, A., Van Montagu, M. and Wang, K. (1990). A bacterial peptide acting as a plant nuclear targeting signal: the amincπterminal portion of Agrobacterium VirD2 protein directs a β-galactosidase fusion protein into tobacco nuclei. Proceedings of the National Academy of Sciences USA, 87, 9534-9537.
129. Hiei, Y., Ohta, S., Komari, T. and Kumashiro, T. (1994). Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal, 6, 271-282.
130. Hille, J., Verheggen, F., Roelvink, P., Franssen, H., van Kammen, A. and Zabel, P. (1986). Bleomycin resistance: a new dominant selectable marker for plant cell transformation. Plant Molecular Biology, 7, 171-176.
131. Hoeijmakers, J.H.J, and Bootsma, D. (1994). Incisions for excision. Nature, 371, 654-655.
132. Hoekema, A., Hirsch, P.R., Hooykaas, P.J.J. and Schilperoort, R.A. (1983). A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature, 303, 179-180.
133. Holsters, M., Villarroel, R., Van Montagu, M. and Schell, J. (1982). The use of selectable markers for the isolation of plant-DNA/T-DNA junction fragments in a cosmid vector. Molecular and General Genetics, 185, 283-289.
134. Holsters, M., Villarroel, R., Gielen, J., Seurinck, J., De Greve, H., Van Montagu, M. and Schell, J. (1983). An analysis of the boundaries of the octopine TL-DNA in tumors induced by Agrobacterium tumefaciens. Molecular and General Genetics, 190, 35-41.
135. Hood, E.E., Fraley, R.T. and Chilton, M.-D. (1987). Virulence of Agrobacterium tumefaciens strain A281 on legumes. Plant Physiology, 83, 529-534.
136. Hooykaas, P.J.J, and Beijersbergen, A.G.M. (1994). The virulence system of Agrobacterium tumefaciens. Annual Review of Phytopathology, 32, 157-179.
137. Howard, E.A., Zupan, J.R., Citovsky, V. and Zambryski, P.C. (1992). The VirD2 protein of A. tumefaciens contains a C-terminal bipartite nuclear localization signal: implications for nuclear uptake of DNA in plant cells. Cell, 68, 109-118.
138. Huang, M.-L.W., Cangelosi, G.A., Halperin, W. and Nester, E.W. (1990). A chromosomal Agrobacterium tumefaciens gene required for effective plant signal transduction. Journal of Bacteriology, 172, 1814-1822.
139. Huffman, G.A., White, F.F., Gordon, M.P. and Nester, E.W. (1984). Hairy-root-inducing plasmid: physical map and homology to tumor-inducing plasmids. Journal of Bacteriology, 157, 269-276.
140. Ishida, Y., Saito, H., Ohta, S., Hiei, Y., Komari, T. and Kumashiro, T. (1996). High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens. Nature Biotechnology, 14, 745-750.
141. Janssen, B.-J. and Gardner, R.C. (1989). Localized transient expression of GUS in leaf discs following cocultivation with Agrobacteńum. Plant Molecular Biology, 14, 61-72.
142. Jarchow, E., Grimsley, N.H. and Hohn, B. (1991). virF, the host-range-determining virulence gene of Agrobacterium tumefaciens, affects T-DNA transfer to Zea mays. Proceedings of the National Academy of Sciences USA, 88, 10426-10430.
143. Jasper, F., Koncz, C, Schell, J. and Steinbiss, H.-H. (1994). Agrobacterium T-strand production in vitro: sequence-specific cleavage and 5' protection of single-stranded DNA templates by purified VirD2 protein. Proceedings of the National Academy of Sciences USA, 91, 694-698.
144. Jayaswal, R.K., Veluthambi, K., Gelvin, S.B. and Slightom, J.L. (1987). Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a rørD-encoded border-specific endonuclease from Agrobacterium tumefaciens. Journal of Bacteriology, 169, 5035-5045.
145. Jen, G.C. and Chilton, M.-D. (1986). The right border region of ρTiT37 T-DNA is intrinsically more active than the left border region in promoting T-DNA transformation. Proceedings of the National Academy of Sciences USA, 83, 3895-3899.
146. Jin, S., Komari, T., Gordon, M.P. and Nester, E.W. (1987). Genes responsible for the supervirulence phenotype of Agrobacteńum tumefaciens A281. Journal of Bacteriology, 169, 4417-4425.
147. Jin, S., Prusti, R.K., Roitsch, T., Ankenbauer, R.G. and Nester, E.W. (1990a). Phosphorylation of the VirG protein of Agrobacterium tumefaciens by the autophos-phorylated VirA protein: essential role in biological activity of VirG. Journal of Bacteriology, 172, 4945-4950.
148. Jin, S., Roitsch, T., Christie, P.J. and Nester, E.W. (1990b). The regulatory VirG protein specifically binds to a cis-acting regulatory sequence involved in transcriptional activation of Agrobacterium tumefaciens virulence genes. Journal of Bacteriology, 172, 531-537.
149. Joersbo, M. and Okkels, F.T. (1996). A novel principle for selection of transgenic plant cells: positive selection. Plant Cell Reports, 16, 219-221.
150. Jones, A.L., Lai, E.-M., Shirasu, K. and Kado, C.I. (1996). VirB2 is a processed pilin-like protein encoded by the Agrobacterium tumefaciens Ti plasmid. Journal of Bacteriology, 178, 5706-5711.
151. Jones, J.D.G., Gilbert, D.E., Grady, K.L. and Jorgensen, R.A. (1987). T-DNA structure and gene expression in petunia plants transformed by Agrobacterium tumefaciens C58 derivatives. Molecular and General Genetics, 207, 478-485.
152. Joos, H., Timmerman, B., Van Montagu, M. and Schell, J. (1983). Genetic analysis of transfer and stabilization of Agrobacterium DNA in plant cells. The EMBO Journal, 2, 2151-2160.
153. Jordan, M.C. and Hobbs, S.L.A. (1993). Evaluation of a cotyledonary node regeneration system for Agrobacterium-mediated transformation of pea (Pisum sativum L.). In Vitro Cellular and Developmental Biology, 29P, 77-82.
154. Jorgensen, R., Snyder, C. and Jones, J.D.G. (1987). T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobacterium tumefaciens C58 derivatives. Molecular and General Genetics, 207, 471-477.
155. Kado, C.I. (1994). Promiscuous DNA transfer system of Agrobacterium tumefaciens: role of the virB operon in sex pilus assembly and synthesis. Molecular Microbiology, 12, 17-22.
156. Kalogeraki, V.S. and Winans, S.C. (1995). The octopine-type Ti plasmid pTiA6 of Agrobacterium tumefaciens contains a gene homologous to the chromosomal virulence gene acvB. Journal of Bacteriology, 177, 892-897.
157. Kamakura, T., Yoneyama, K. and Yamaguchi, I. (1990). Expression of the blasticidin S deaminase gene (bsr) in tobacco: fungicide tolerance and a new selective marker for transgenic plants. Molecular and General Genetics, 223, 332-334.
158. Kanemoto, R.H., Powell, A.T., Akiyoshi, D.E., Regier, D.A., Kerstetter, R.A., Nester, E.W., Hawes, M.C. and Gordon, M.P. (1989). Nucleotide sequence and analysis of the plant-inducible locus pinF from Agrobacterium tumefaciens. Journal of Bacteriology, 171, 2506-2512.
159. Kapila, J., De Rycke, R., Van Montagu, M. and Angenon, G. (1997). An Agrobacterium-mediated transient gene expression system for intact leaves. Plant Science, 122, 101-108.
160. Karp, A. (1991). On the current understanding of somac-lonal variation. Oxford Surveys of Plant Molecular and Cell Biology, 7, 1-58.
161. Kata vie, V., Haughn, G. W., Reed, D., Martin, M. and Kunst, L. (1994). In planta transformation of Arabidopsis thaliana. Molecular and General Genetics, 245, 363-370.
162. Kertbundit, S., De Greve, H., Deboeck, F., Van Montagu, M. and Hernalsteens, J.-P. (1991). In vivo random ^-glucuronidase gene fusions in Arabidopsis thaliana. Proceedings of the National Academy of Sciences USA, 88, 5212-5216.
163. Knittel, N., Gruber, V., Hahne, G. and Lénée, P. (1994). Transformation of sunflower (Helianthus annuus L.): a reliable protocol. Plant Cell Reports, 14, 81-86.
164. Köhler, F., Cardon, G, Pöhlman, M., Gül, R. and Schieder, O. (1989). Enhancement of transformation rates in higher plants by low-dose irradiation: are DNA repair systems involved in the incorporation of exogenous DNA into the plant genome? Plant Molecular Biology, 12, 189-199.
165. L-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector. Molecular and General Genetics, 204, 383-396.
166. Koncz, C, Martini, N., Mayerhofer, R., Koncz-Kalman, Z., Körber, H., Redei, GP. and Schell, J. (1989). High-frequency T-DNA-mediated gene tagging in plants. Proceedings of the National Academy of Sciences USA, 86, 8467-8471.
167. Kondorosi, A. (1992). Regulation of nodulation genes in rhizobia. In Molecular Signals in Plant-Microbe Communications, edited by D.P.S. Verma, pp. 325-340. Boca Raton: CRC Press.
168. Kononov, M.E., Bassuner, B. and Gelvin, S.B. (1997). Integration of T-DNA binary vector 'backbone' sequences into the tobacco genome: evidence for multiple complex patterns of integration. The Plant Journal, 11, 945-957.
169. Körber, H., Strizhov, N., Staiger, D., Feldwisch, J., Olsson, O., Sandberg, G., Palme, K., Schell, J. and Koncz, C. (1991). T-DNA gene 5 of Agrobacterium modulates auxin response by autoregulated synthesis of a growth hormone antagonist in plants. The EMBO Journal, 10, 3983-3991.
170. Koukoliková-Nicola, Z., Raineri, D., Stephens, K., Ramos, G, Tinland, B., Nester, E.W. and Hohn, B. (1993). Genetic analysis of the virD Operon of Agrobactenum tumefaciens: a search for functions involved in transport of T-DNA into the plant cell nucleus and in T-DNA integration. Journal of Bacteriology, 175, 723-731.
171. Krysan, P.J., Young, J.C., Tax, F. and Sussman, M.R. (1996). Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport. Proceedings of the National Academy of Sciences USA, 93, 8145-8150.
172. Kwok, W.W., Nester, E.W. and Gordon, M.P. (1985). Unusual plasmid DNA organization in an octopine crown gall tumor. Nucleic Acids Research, 13, 459-471.
173. Lee, K., Dudley, M.W., Hess, K.M., Lynn, D.G., Joerger, R.D. and Binns, A.N. (1992). Mechanism of activation of Agrobacterium virulence genes: identification of phenol-binding proteins. Proceedings of the National Academy of Sciences USA, 89, 8666-8670.
174. Lee, Y.-W., Jin, S., Sim, W.-S. and Nester, E.W. (1995). Genetic evidence for direct sensing of phenolic compounds by the VirA protein of Agrobacterium tumefaciens. Proceedings of the National Academy of Sciences USA, 92, 12245-12249.
175. Leroux, B., Yanofsky, M.F., Winans, S.C., Ward, J.E., Ziegler, S.F. and Nester, E.W. (1987). Characterization of the vir A locus of Agrobacterium tumefaciens: a transcriptional regulator and host range determinant. The EMBO Journal, 6, 849-856.
176. Lessl, M. and Lanka, E. (1994). Common mechanisms in bacterial conjugation and Ti-mediated T-DNA transfer to plant cells. Cell, 77, 321-324.
177. Lessl, M., Balzer, D., Pansegrau, W. and Lanka, E. (1992). Sequence similarities between the RP4 Tra2 and the Ti VirB region strongly support the conjugation model for T-DNA transfer. Journal of Biological Chemistry, 267, 20471-20480.
178. Li, X.-Q., Liu, C.-N., Ritchie, S.W., Peng, J.-y., Gelvin, S.B. and Hodges, T.K. (1992). Factors influencing Agrobacterium-mediated transient expression of gusA in rice. Plant Molecular Biology, 20, 1037-1048.
179. Lin, T.-S. and Kado, C.I. (1993). The virD4 gene is required for virulence while virD3 and orf5 are not required for virulence of Agrobacterium tumefaciens. Molecular Microbiology, 9, 803-812.
180. Liu, C.-N., Li, X.-Q. and Gelvin, S.B. (1992). Multiple copies of virG enhance the transient transformation of celery, carrot and rice tissues by Agrobacterium tumefaciens. Plant Molecular Biology, 20, 1071-1087.
181. Liu, Y.-G, Mitsukawa, N., Oosumi, T. and Whittier, R.F. (1995). Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. The Plant Journal, 8, 457-463.
182. Lowe, K., Bowen, B., Hoerster, G, Ross, M., Bond, D., Pierce, D. and Gordon-Kamm, B. (1995). Germline transformation of maize following manipulation of chimeric shoot meristems. Bio/technology, 13, 677-682.
183. Maliga, P., Svab, Z., Harper, E.C. and Jones, J.D.G. (1988). Improved expression of streptomycin resistance in plants due to a deletion of the streptomycin phosphotransferase coding sequence. Molecular and General Genetics, 214, 456-459.
184. Malone-Schoneberg, J., Scelonge, C.J., Burrus, M. and Bidney, D.L. (1994). Stable transformation of sunflower using Agrobacterium and split embryonic axis expiants. Plant Science, 103, 199-207.
185. Mantis, N.J. and Winans, S.C (1993). The chromosomal response regulatory gene chvl of Agrobacterium tumefaciens complements an Escherichia coli phoB mutation and is required for virulence. Journal of Bacteriology, 175, 6626-6636.
186. Martineau, B., Voelker, T.A. and Sanders, R.A. (1994). On defining T-DNA. The Plant Cell, 6, 1032-1033.
187. Marton, L., Hrouda, M., Pécsváradi, A. and Czakó, M. (1994). T-DNA-insert-independent mutations induced in transformed plant cells during Agrobacterium co-cultivation. Transgenic Research, 3, 317-325.
188. Matsumoto, S., Ito, Y., Hosoi, T., Takahashi, Y. and Machida, Y. (1990). Integration of Agrobacterium T-DNA into a tobacco chromosome: possible involvement of DNA homology between T-DNA and plant DNA. Molecular and General Genetics, 224, 309-316.
189. Matthysse, A.G. (1987). Characterization of nonattaching mutants of Agrobacterium tumefaciens. Journal of Bacteriology, 169, 313-323.
190. Matthysse, A.G., Yarnall, H.A. and Young, N. (1996). Requirement for genes with homology to ABC transport systems for attachment and virulence of Agrobacterium tumefaciens. Journal of Bacteriology, 178, 5302-5308.
191. May, G.D., Afza, R, Mason, H.S., Wiecko, A., Novak, F.J. and Arntzen, C.J. (1995). Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation. Bio/technology, 13, 486-492.
192. Mayerhofer, R., Koncz-Kalman, Z., Nawrath, C, Bakkeren, G., Crameri, A., Angelis, K., Redei, G.P., Schell, J., Hohn, B. and Koncz, C. (1991). T-DNA integration: a mode of illegitimate recombination in plants. The EMBO Journal, 10, 697-704.
193. McCabe, D.E. and Martinell, B.J. (1993). Transformation of elite cotton cultivars via particle bombardment of meristems. Bio/technology, 11, 596-598.
194. McCabe, D.E., Swain, W.F., Martinell, B.J. and Christou, P. (1988). Stable transformation of soybean (Glycine max) by particle acceleration. Bio/technology, 6, 923-926.
195. McKinney, E.C., Ali, N., Traut, A., Feldmann, K.A., Belostotsky, D.A., McDowell, J.M. and Meagher, R.B. (1995). Sequence-based identification of T-DNA insertion mutations in Arabidopsis: actin mutants actl-l and act4-l. The Plant Journal, 8, 613-622.
196. McKnight, T.D., Lillis, M.T. and Simpson, R.B. (1987). Segregation of genes transferred to one plant cell from two separate Agrobacterium strains. Plant Molecular Biology, 8, 439-445.
197. Mehlin, H., Daneholt, B. and Skoglund, U. (1992). Translocation of a specific premessenger ribonucleoprotein particle through the nuclear pore studied with electron microscope tomography. Cell, 69, 605-613.
198. Melchers, L.S., Thompson, D.V., Idler, K.B., Schilperoort, R.A. and Hooykaas, P.J.J. (1986). Nucleotide sequence of the virulence gene virG of the Agrobacterium tumefaciens octopine Ti plasmid: significant homology between virG and the regulatory genes ompR, phoB, and dye of E. coli. Nucleic Acids Research, 14, 9933-9940.
199. Melchers, L.S., Regensburg-Tuink, J.G., Bourret, R.B., Sedee, N.J.A., Schilperoort, R.A. and Hooykaas, P.J.J. (1989). Membrane topology and functional analysis of the sensory protein VirA of Agrobacterium tumefaciens. The EMBO Journal, 8, 1919-1925.
200. Melchers, L.S., Maroney, M.J., den Dulk-Ras, A., Thompson, D.V., van Vuuren, H.A.J., Schilperoort, R.A. and Hooykaas, P.J.J. (1990). Octopine and nopa-line strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus. Plant Molecular Biology, 14, 249-259.
201. Menage, A. and Morel, G. (1965). Sur la présence d'un acide aminé nouveau dans le tissu de crown-gall. Comptes Rendus de la Société de Biologie (Paris), 159, 561-562.
202. Mersereau, M., Pazour, G.J. and Das, A. (1990). Efficient transformation of Agrobacterium tumefaciens by elec-troporation. Gene, 90, 149-151.
203. Messens, E., Dekeyser, R. and Stachel, S. (1990). A non-transformable Triticum monococcum monocotyledonous culture produces the potent Agrobacterium wr-inducing compound ethyl ferulate. Proceedings of the National Academy of Sciences USA, 87, 4368-4372.
204. Metts, J., West, J., Doares, S.H. and Matthysse, A.G. (1991). Characterization of three Agrobacterium tumefaciens avirulent mutants with chromosomal mutations that affect induction of vir genes. Journal of Bacteriology, 173, 1080-1087.
205. Meyer, P. (1995). Gene Silencing in Higher Plants and Related Phenomena in Other Eukaryotes (Current Topics in Microbiology and Immunology, Vol. 197). Berlin: Springer-Verlag.
206. Miao, Z.-H. and Lam, E. (1995). Targeted disruption of the TGA3 locus in Arabidopsis thaliana. The Plant Journal, 7, 359-365.
207. Miranda, A., Janssen, G., Hodges, L., Peralta, E.G. and Ream, W. (1992). Agrobacterium tumefaciens transfers extremely long T-DNAs by a unidirectional mechanism. Journal of Bacteriology, 174, 2288-2297.
208. Mlynárová, L., Loonen, A., Heidens, J., Jansen, R.C., Keizer, P., Stiekema, W.J. and Nap, J.-P. (1994). Reduced position effect in mature transgenic plants conferred by the chicken lysozyme matrix-associated region. The Plant Cell, 6, 417-426.
209. Morris, J.W. and Morris, R.O. (1990). Identification of an Agrobacterium tumefaciens virulence gene inducer from the pinaceous gymnosperm Pseudotsuga menziesii. Proceedings of the National Academy of Sciences USA, 87, 3614-3618.
210. Müller, A.J., Mendel, R.R., Schiemann, J., Simoens, C. and Inzé, D. (1987). High meiotic stability of a foreign gene introduced into tobacco by Agrobacterium-mediated transformation. Molecular and General Genetics, 207, 171-175.
211. Mushegian, A.R., Fullner, K.J., Koonin, E.V. and Nester, E.W. (1996). A family of lysozyme-like virulence factors in bacterial pathogens of plants and animals. Proceedings of the National Academy of Sciences USA, 93, 7321-7326.
212. Nam, J., Matthysse, A.G. and Gelvin, S.B. (1997). Differences in susceptibility of Arabidopsis ecotypes to crown gall disease may result from a deficiency in T-DNA integration. The Plant Cell, 9, 317-333.
213. Narasimhulu, S.B., Deng, X.-b., Sarria, R. and Gelvin, S.B. (1996). Early transcription of Agrobacterium T-DNA genes in tobacco and maize. The Plant Cell, 8, 873-886.
214. Nauerby, B., Billing, K. and Wyndaele, R. (1997). Influence of the antibiotic timentin on plant regeneration compared to carbenicillin and cefotaxime in concentrations suitable for elimination of Agrobacterium tumefaciens. Plant Science, 123, 169-177.
215. Odell, J.T. and Russell, S.H. (1994). Use of site-specific recombination systems in plants. In Homologous Recombination and Gene Silencing in Plants, edited by J. Paszkowski, pp. 219-270. Dordrecht: Kluwer Academic Publishers.
216. Offringa, R., de Groot, M.J.A., Haagsman, HJ., Does, M.P., van den Elzen, P. and Hooykaas, P.J.J. (1990). Extrachromosomal homologous recombination and gene targeting in plant cells after Agrobacterium mediated transformation. The EMBO Journal, 9, 3077-3084.
217. v Franke-Van Dijk, M.E.L, de Groot, M.J.A., van den Elzen, P.J.M. and Hooykaas, P.J.J. (1993). Nonreciprocal homologous recombination between Agrobacterium transferred DNA and a plant chromosomal locus. Proceedings of the National Academy of Sciences USA, 90, 7346-7350.
218. Ohba, T., Yoshioka, Y., Machida, C. and Machida, Y. (1995). DNA rearrangement associated with the integration of T-DNA in tobacco: an example for multiple duplications of DNA around the integration target. The Plant Journal, 7, 157-164.
219. Ohl, S., Offringa, R., Van den Elzen, P.J.M. and Hooykaas, P.J.J. (1994). Gene replacement in plants. In Homologous Recombination and Gene Silencing in Plants, edited by J. Paszkowski, pp. 191-217. Dordrecht, Kluwer Academic Publishers
220. Okamoto, S., Toyada-Yamamoto, A., Ito, K., Takebe, I. and Machida, Y. (1991). Localization and orientation of the VirD4 protein of Agrobactenum tumefaciens in the cell membrane. Molecular and General Genetics, 228, 24-32.
221. Otten, L., De Greve, H., Leemans, J., Hain, R., Hooykaas, P.J.J. and Schell, J. (1984). Restoration of virulence of Vir region mutants of Agrobacterium tumefaciens strain B6S3 by coinfection with normal and mutant Agrobactenum strains. Molecular and General Genetics, 195, 159-163.
222. Pan, S.Q., Charles, T., Jin, S., Wu, Z.-L. and Nester, E.W. (1993). Preformed dimeric state of the sensor protein VirA is involved in plant-Agrobacterium signal transduction. Proceedings of the National Academy of Sciences USA, 90, 9939-9943.
223. Pan, S.Q., Jin, S., Boulton, M.I., Hawes, M., Gordon, M.P. and Nester, E.W. (1995). An Agrobacterium virulence factor encoded by a Ti plasmid gene or a chromosomal gene is required for T-DNA transfer into plants. Molecular Microbiology, 17, 259-269.
224. Pansegrau, W. and Lanka, E. (1991). Common sequence motifs in DNA relaxases and nick regions from a variety of DNA transfer systems. Nucleic Acids Research, 19, 3455.
225. Pansegrau, W., Schoumacher, F., Hohn, B. and Lanka, E. (1993). Site-specific cleavage and joining of single-stranded DNA by VirD2 protein of Agrobacterium tumefaciens Ti plasmids: analogy to bacterial conjugation. Proceedings of the National Academy of Sciences USA, 90, 11538-11542.
226. Pansegrau, W., Lanka, E., Barth, P.T., Figurski, D.H., Guiney, D.G, Haas, D., Helinski, D.R., Schwab, H., Stanisich, V.A. and Thomas, CM. (1994a). Complete nucleotide sequence of Birmingham IncPα plasmids. Compilation and comparative analysis. Journal of Molecular Biology, 239, 623-663.
227. Pansegrau, W., Schröder, W. and Lanka, E. (1994b). Concerted action of three distinct domains in the DNA cleaving-joining reaction catalyzed by relaxase (Tral) of conjugative plasmid RP4. Journal of Biological Chemistry, 269, 2782-2789.
228. Park, S.H., Pinson, S.R.M. and Smith, R.H. (1996). T-DNA integration into genomic DNA of rice following Agrobacterium inoculation of isolated shoot apices. Plant Molecular Biology, 32, 1135-1148.
229. Parke, D., Ornston, L.N. and Nester, E.W. (1987). Chemotaxis to plant phenolic inducers of virulence genes is constitutively expressed in the absence of the Ti plasmid in Agrobacterium tumefaciens. Journal of Bacteriology, 169, 5336-5338.
230. Peach, C. and Velten, J. (1991). Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters. Plant Molecular Biology, 17, 49-60.
231. Peerbolte, R., Ruigrok, P., Wullems, G. and Schilperoort, R. (1987). T-DNA rearrangements due to tissue culture: somaclonal variation in crown gall tissues. Plant Molecular Biology, 9, 51-57.
232. Peralta, E.G. and Ream, L.W. (1985). T-DNA border sequences required for crown gall tumorigenesis. Proceedings of the National Academy of Sciences USA, 82, 5112-5116.
233. Peralta, E.G., Hellmiss, R. and Ream, W. (1986). Oz;er-drive, a T-DNA transmission enhancer on the A. tumefaciens tumour-inducing plasmid. The EMBO Journal, 5, 1137-1142.
234. Perez, P., Tiraby, G, Kallerhoff, J. and Perret, J. (1989). Phleomycin resistance as a dominant selectable marker for plant cell transformation. Plant Molecular Biology, 13, 365-373.
235. Perl, A., Galili, S., Shaul, O., Ben-Tzvi, I. and Galili, G. (1993). Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase: two novel selectable markers for plant transformation. Bio/technology, 11, 715-718.
236. Perl, A., Lotan, O., Abu-Abied, M. and Holland, D. (1996). Establishment of an Agrobacterium-mediated transformation system for grape (Vitis viniferα L.): the role of antioxidants during grape-Agrobαcterium interactions. Nature Biotechnology, 14, 624-628.
237. Pickardt, T., Saalbach, L, Waddell, D., Meixner, M., Müntz, K. and Schieder, O. (1995). Seed specific expression of the 2S albumin gene from Brazil nut (Bertholletia excelsa) in transgenic Vicia narbonensis. Molecular Breeding, 1, 295-301.
238. Piers, K.L., Heath, J.D., Liang, X., Stephens, K.M. and Nester, E.W. (1996). Agrobacterium tumefaciens-mediated transformation of yeast. Proceedings of the National Academy of Sciences USA, 93, 1613-1618.
239. Pohlman, R.F., Genetti, H.D. and Winans, S.C (1994). Common ancestry between IncN conjugal transfer genes and macromolecular export systems of plant and animal pathogens. Molecular Microbiology, 14, 655-668.
240. Powell, B.S. and Kado, C.I. (1990). Specific binding of VirG to the vir box requires a C-terminal domain and exhibits a mimimum concentration threshold. Molecular Microbiology, 4, 2159-2166.
241. Pröls, F. and Meyer, P. (1992). The methylation pattern of chromosomal integration regions influence gene activity of transferred DNA in Petunia hybrida. The Plant Journal, 2, 465-475.
242. Puchta, H., Dujon, B. and Hohn, B. (1996). Two different but related mechanisms are used in plants for the repair of genomic double-strand breaks by homologous recombination. Proceedings of the National Academy of Sciences USA, 93, 5055-5060.
243. L-DNA. Plant Molecular Biology, 28, 1149-1154.
244. Rashkova, S., Spudich, G.M. and Christie, P.J. (1997). Characterization of membrane and protein interaction determinants of the Agrobacterium tumefaciens VirBll ATPase. Journal of Bacteriology, 179, 583-591.
245. Regensburg-Tuink, A.J.G. and Hooykaas, P.J.J. (1993). Transgenic N. glauca plants expressing bacterial virulence gene virF are converted into hosts for nopa-line strains of A. tumefaciens. Nature, 363, 69-71.
246. Renckens, S., De Greve, H., Van Montagu, M. and Her-nalsteens, J.-P. (1992). Petunia plants escape from negative selection against a transgene by silencing the foreign DNA via methylation. Molecular and General Genetics, 233, 53-64.
247. Risseeuw, E., Offringa, R., Franke-van Dijk, M.E.I, and Hooykaas, P.J.J. (1995). Targeted recombination in plants using Agrobacterium coincides with additional rearrangements at the target locus. The Plant Journal, 7, 109-119.
248. Risseeuw, E., Franke-van Dijk, M.E.I, and Hooykaas, P.J.J. (1997). Gene targeting and instability of Agrobacterium T-DNA loci in the plant genome. The Plant Journal, 11, 717-728.
249. Risuleo, G, Baltistoni, P. and Costantino, P. (1982). Regions of homology between tumorigenic plasmids of Agrobacterium rhizogenes and Agrobacterium tumefaciens. Plasmid, 7, 45-51.
250. Ritala, A., Aspegren, K., Kurten, U., Salmenkallio-Marttila, M., Mannonen, L., Hannus, R., Kauppinen, V., Teeri, T.H. and Enari, T.-M. (1994). Fertile transgenic barley by particle bombardment of immature embryos. Plant Molecular Biology, 24, 317-325.
251. Robbins, T.P., Geräts, A.G.M., Fiske, H. and Jorgensen, R.A. (1995). Suppression of recombination in wide hybrids of Petunia hybrida as revealed by genetic mapping of marker transgenes. Theoretical and Applied Genetics, 90, 957-968.
252. Rogowsky, P.M., Close, T.J., Chimera, J.A., Shaw, J.J. and Kado, C.I. (1987). Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58. Journal of Bacteriology, 169, 5101-5112.
253. Rossi, L., Hohn, B. and Tinland, B. (1993). The VirD2 protein of Agrobacterium tumefaciens carries nuclear localization signals important for transfer of T-DNA to plants. Molecular and General Genetics, 239, 345-353.
254. Rossi, L., Hohn, B. and Tinland, B. (1996). Integration of complete transferred DNA units is dependent on the activity of virulence E2 protein of Agrobacterium tumefaciens. Proceedings of the National Academy of Sciences USA, 93, 126-130.
255. Roth, D.B. and Wilson, J.H. (1986). Nonhomologous recombination in mammalian cells: role for short sequence homologies in the joining reaction. Molecular and Cellular Biology, 6, 4295-4304.
256. Roth, D.B., Chang, W.-B. and Wilson, J.H. (1989). Comparison of filler DNA at immune, nonimmune, and oncogenic rearrangements suggests multiple mechanisms of formation. Molecular and Cellular Biology, 9, 3049-3057.
257. Russell, D.R., Wallace, K.M., Bathe, J.H., Martinell, B.J. and McCabe, D.E. (1993). Stable transformation of Phaseolus vulgaris via electric-discharge mediated particle acceleration. Plant Cell Reports, 12, 165-169.
258. Sahi, S.V., Chilton, M.-D. and Chilton, W.S. (1990). Corn metabolites affect growth and virulence of Agrobacterium tumefaciens. Proceedings of the National Academy of Sciences USA, 87, 3879-3883.
259. Sangwan, R.S., Bourgeois, Y., Brown, S., Vasseur, G. and Sangwan-Norreel, B. (1992). Characterization of competent cells and early events of Agrobacterium-mediated genetic transformation in Arabidopsis thaliana. Planta, 188, 439-456.
260. Satina, S., Blakeslee, A.F. and Avery, A.G. (1940). Demonstration of the three germ layers in the shoot apex of Datura by means of induced polyploidy in peric-linal chimeras. American Journal of Botany, 27, 895-905.
261. Scheeren-Groot, E.P., Rodenburg, K.W., den Dulk-Ras, A., Turk, S.CH.J. and Hooykaas, P.J.J. (1994). Mutational analysis of the transcriptional activator VirG of Agrobacterium tumefaciens. Journal of Bacteriology, 176, 6418-6426.
262. Scheiffele, P., Pansegrau, W. and Lanka, E. (1995). Initiation of Agrobacterium tumefaciens T-DNA processing. Purified proteins VirDl and VirD2 catalyze site- and strand-specific cleavage of superhelical T-border DNA in vitro. Journal of Biological Chemistry, 270, 1269-1276.
263. Schell, J., Van Montagu, M., De Beuckeleer, M., De Block, M., Depicker, A., De Wilde, M., Engler, G., Genetello, C, Hernalsteens, J.P., Holsters, M., Seurinck, J., Silva, B., Van Vliet, F. and Villarroel, R. (1979). Interactions and DNA transfer between Agrobacterium tumefaciens, the Ti-plasmid and the plant host. Proceedings of the Royal Society of London B 204, 251-266.
264. Schöffl, F., Schröder, G., Kliem, M. and Rieping, M. (1993). An SAR sequence containing 395 bp DNA fragment mediates enhanced, gene-dosage-correlated expression of a chimaeric heat shock gene in transgenic tobacco plants. Transgenic Research, 2, 93-100.
265. Schrammeijer, B., Sijmons, P.C., van den Elzen, P.J.M. and Hoekema, A. (1990). Meristem transformation of sunflower via Agrobacterium. Plant Cell Reports, 9, 55-60.
266. Schroeder, H.E., Schotz, A.H., Wardley-Richardson, T., Spencer, D. and Higgins, T.J.V. (1993). Transformation and regeneration of two cultivars of pea (Pisum sativum L.). Plant Physiology, 101, 751-757.
267. Scofield, S.R., Tobias, CM., Rathjen, J.P., Chang, J.H., Lavelle, D.T., Michelmore, R.W. and Staskawicz, B.J. (1996). Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato. Science, 274, 2063-2065.
268. Shackelford, N.J. and Chlan, C.A. (1996). Identification of antibiotics that are effective in eliminating Agrobacterium tumefaciens. Plant Molecular Biology Reporter, 14, 50-57.
269. Shaw, CH., Ashby, A.M., Brown, A., Royal, C, Loake, G.J. and Shaw, CH. (1988). Vir A and virG are the Ti-plasmid functions required for Chemotaxis of Agrobacterium tumefaciens towards acetosyringone. Molecular Microbiology, 2, 413-417.
270. Shimoda, N., Toyoda-Yamamoto, A., Nagamine, J., Usami, S., Katayama, M., Sakagami, Y. and Machida, Y. (1990). Control of expression of Agrobactenum vir genes by synergistic action of phenolic signal molecules and monosaccharides. Proceedings of the National Academy of Sciences USA, 87, 6684-6688.
271. Shimoda, N., Toyoda-Yamamoto, A., Aoki, S. and Machida, Y. (1993). Genetic evidence for an interaction between the VirA sensor protein and the ChvE sugar-binding protein of Agrobacterium. Journal of Biological Chemistry, 268, 26552-26558.
272. Shirasu, K. and Kado, C.I. (1993a). Membrane location of the Ti plasmid VirB proteins involved in the biosynthesis of a pilin-like conjugative structure on Agrobactenum tumefaciens. FEMS Microbiology Letters, 111, 287-294.
273. Shirasu, K. and Kado, C.I. (1993b). The virB operon of the Agrobacterium tumefaciens virulence regulon has sequence similarities to B, C and D open reading frames downstream of the pertussis toxin-operon and to the DNA transfer-operons of broad-host-range conjugative plasmids. Nucleic Acids Research, 21, 353-354.
274. Shirasu, K., Morel, P. and Kado, C.I. (1990). Characterization of the virB operon of an Agrobacterium tumefaciens Ti plasmid: nucleotide sequence and protein analysis. Molecular Microbiology, 4, 1153-1163.
275. Shirasu, K., Koukolíková-Nicola, Z., Hohn, B. and Kado, C.I. (1994). An inner-membrane-associated virulence protein essential for T-DNA transfer from Agrobacterium tumefaciens to plants exhibits ATPase activity and similarities to conjugative transfer genes. Molecular Microbiology, 11, 581-588.
276. Shurvinton, CE., Hodges, L. and Ream, W. (1992). A nuclear localization signal and the C-terminal omega sequence in the Agrobacterium tumefaciens VirD2 endo-nuclease are important for tumor formation. Proceedings of the National Academy of Sciences USA, 89, 11837-11841.
277. Simpson, R.B., O'Hara, P.J., Kwok, W., Montoya, A.L., Lichtenstein, C, Gordon, M.P. and Nester, E.W. (1982). DNA from the A6S/2 crown gall tumors contains scrambled Ti-plasmid sequences near its junctions with the plant DNA. Cell, 29, 1005-1014.
278. 2+-dependent adhesin, which mediates the first step in attachment of cells of the family Rhizobiaceae to plant root hair tips. Journal of Bacteriology, 171, 4054-4062.
279. Smith, R.H. and Murashige, T. (1970). In vitro development of the isolated shoot apical meristem of angios-perms. American Journal of Botany, 57, 562-568.
280. Smith, D., Yanai, Y., Liu, Y.-G, Ishiguro, S., Okada, K., Shibata, D., Whittier, R.F. and Fedoroff, N.V. (1996). Characterization and mapping of Ds-GUS-T-DNA lines for targeted insertional mutagenesis. The Plant Journal, 10, 721-732.
281. Sonti, R.V., Chiurazzi, M., Wong, D., Davies, CS., Harlow, G.R., Mount, D.W. and Signer, E.R. (1995). Arabidopsis mutants deficient in T-DNA integration. Proceedings of the Nationl Academy of Sciences USA, 92, 11786-11790.
282. Spanier, K., Schell, J. and Schreier, P.H. (1989). A functional analysis of T-DNA gene 6b: the fine tuning of cytokinin effects on shoot development. Molecular and General Genetics., 219, 209-216.
283. Spencer, P.A. and Towers, G.H.N. (1988). Specificity of signal compounds detected by Agrobacterium tumefaciens. Phytochemistry, 27, 2781-2785.
284. Spielmann, A. and Simpson, R.B. (1986). T-DNA structure in transgenic tobacco plants with multiple independent integration sites. Molecular and General Genetics, 205, 34-41.
285. Spudich, G.M., Fernandez, D., Zhou, X.-R. and Christie, P.J. (1996). Intermolecular disulfide bonds stabilize VirB7 homodimers and VirB7/VirB9 heterodimers during biogenesis of the Agrobacterium tumefaciens T-complex transport apparatus. Proceedings of the National Academy of Sciences USA, 93, 7512-7517.
286. Stachel, S.E. and Nester, E.W. (1986). The genetic and transcriptional organization of the vir region of the A4 Ti plasmid of Agrobacterium tumefaciens. The EMBO Journal, 5, 1445-1454.
287. Stachel, S.E. and Zambryski, P.C. (1986a). vir A and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciens. Cell, 46, 325-333.
288. Stachel, S.E. and Zambryski, P. (1986b). Agrobacterium tumefaciens and the susceptible plant cell: a novel adaptation of extracellular recognition and DNA conjugation. Cell, 47, 155-157.
289. Stachel, S.E., Messens, E., Van Montagu, M. and Zambryski, P. (1985). Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens. Nature, 318, 624-629.
290. Stachel, S.E., Nester, E.W. and Zambryski, P.C. (1986a). A plant cell factor induces Agrobacterium tumefaciens vir gene expression. Proceedings of the National Academy of Sciences USA, 83, 379-383.
291. Stachel, S.E., Timmerman, B. and Zambryski, P. (1986b). Generation of single-stranded T-DNA molecules during the initial stages of T-DNA transfer from Agrobacterium tumefaciens to plant cells. Nature, 322, 706-712.
292. Stachel, S.E., Timmerman, B. and Zambryski, P. (1987). Activation of Agrobacterium tumefaciens vir gene expression generates multiple single-stranded T-strand molecules from the pTiA6 T-region: requirements for 5' virD gene products. The EMBO Journal, 6, 857-863.
293. Steck, T.R., Morel, P. and Kado, C.I. (1988). Vir box sequences in Agrobacterium tumefaciens pTiC58 and A6. Nucleic Acids Research, 8736.
294. Steck, T.R., Close, T.J. and Kado, C.I. (1989). High levels of double-stranded transferred DNA (T-DNA) processing from an intact nopaline Ti plasmid. Proceedings of the National Academy of Sciences USA, 86, 2133-2137.
295. Steck, T.R., Lin, T.-S. and Kado, C.I. (1990). VirD2 gene product from the nopaline plasmid pTiC58 has at least two activities required for virulence. Nucleic Acids Research, 18, 6953-6958.
296. Stephens, K.M., Roush, C. and Nester, E. (1995). Agrobacterium tumefaciens VirBll protein requires a consensus nucleotide-binding site for function in virulence. Journal of Bacteriology, 177, 27-36.
297. Sundberg, C, Meek, L., Carroll, K., Das, A. and Ream, W. (1996). VirEl protein mediates export of the single-stranded DNA-binding protein VirE2 from Agrobacterium tumefaciens into plant cells. Journal of Bacteriology, 178, 1207-1212.
298. Sussex, I.M. (1989). Developmental programming of the shoot meristem. Cell, 56, 225-229.
299. Svab, Z., Harper, E.C., Jones, J.D.G. and Maliga, P. (1990). Aminoglycoside-3"-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum. Plant Molecular Biology, 14, 197-205.
300. Swart, S., Smit, G., Lugtenberg, B.J.J, and Kijne, J.W. (1993). Restoration of attachment, virulence and nodulation of Agrobacterium tumefaciens chvB mutants by rhicadhesin. Molecular Microbiology, 10, 597-605.
301. Swart, S., Lugtenberg, B.J.J., Smit, G. and Kijne, J.W. (1994). Rhicadhesin-mediated attachment and virulence of an Agrobacterium tumefaciens chvB mutant can be restored by growth in a highly osmotic medium. Journal of Bacteriology., 176, 3816-3819.
302. Tang, X., Frederick, R.D., Zhou, J., Halterman, D.A., Jia, Y. and Martin, G.B. (1996). Initiation of plant disease resistance by physical interaction of AvrPto and Pto kinase. Science, 274, 2060-2063.
303. Tempé, J. and Petit, A. (1982). Opine utilization by Agrobacterium. In Molecular Biology of Plant Tumors, edited by G. Kahl and J. Schell, pp. 451-459. New York: Academic Press.
304. Tepfer, D. (1989). Ri T-DNA from Agrobacterium rhi-zogenes: a source of genes having applications in rhi-zosphere biology and plant development, ecology, and evolution. In Plant-Microbe Interactions: Molecular and Genetic Perspectives, Vol. 3, edited by T. Kosuge and E.W. Nester, pp. 294-342. New York, McGraw-Hill Publishing Company.
305. Thomas, CM., Jones, D.A., English, J.J., Carroll, B.J., Bennetzen, J.L., Harrison, K., Burbidge, A., Bishop, G.J. and Jones, J.D.G. (1994). Analysis of the chromosomal distribution of transposon-carrying T-DNAs in tomato using the inverse polymerase chain reaction. Molecular and General Genetics, 242, 573-585.
306. Thomashow, M.F., Karlinsey, J.E., Marks, J.R. and Hurlbert, R.E. (1987). Identification of a new virulence locus in Agrobacterium tumefaciens that affects polysaccharide composition and plant cell attachment. Journal of Bacteriology, 169, 3209-3216.
307. Thompson, D.V., Melchers, L.S., Idler, K.B., Schilperoort, R.A. and Hooykaas, P.J.J. (1988). Analysis of the complete nucleotide sequence of the Agrobacterium tumefaciens virB operon. Nucleic Acids Research, 16, 4621-4636.
308. Thorstenson, Y.R., Kuldau, G.A. and Zambryski, P. (1993). Subcellular localization of seven VirB proteins of Agrobacterium tumefaciens: implications for the formation of a T-DNA transport structure. Journal of Bacteriology, 175, 5233-5241.
309. Timmerman, B., Van Montagu, M. and Zambryski, P. (1988). vir-induced recombination in Agrobacterium. Physical characterization of precise and imprecise T-circle formation. Journal of Molecular Biology, 203, 373-384.
310. Tingay, S., McElroy, D., Kalla, R., Fieg, S., Wang, M., Thornton, S. and Brettell, R. (1997). Agrobacterium tumefaciens-mediated barley transformation. The Plant Journal, 11, 1369-1376.
311. Tinland, B. and Hohn, B. (1995). Recombination between prokaryotic and eukaryotic DNA: integration of Agrobacterium tumefaciens T-DNA into the plant genome. In Genetic Engineering, Vol. 17, edited by J.K. Setlow, pp. 209-229. New York, Plenum Press.
312. Tinland, B., Huss, B., Paulus, F., Bonnard, G. and Otten, L. (1989). Agrobacterium tumefaciens 6b genes are strain-specific and affect the activity of auxin as well as cytokin-in genes. Molecular and General Genetics, 219, 217-224.
313. Tinland, B., Rohfritsch, O., Michler, P. and Otten, L. (1990). Agrobacterium tumefaciens T-DNA gene 6b stimulates rol-induced root formation, permits growth at high auxin concentrations and increases root size. Molecular and General Genetics, 223, 1-10.
314. Tinland, B., Koukolíková-Nicola, Z., Hall, M.N. and Hohn, B. (1992). The T-DNA linked VirD2 protein contains two distinct functional nuclear localization signals. Proceedings of the National Academy of Sciences USA, 89, 7442-7446.
315. Tinland, B., Hohn, B. and Puchta, H. (1994). Agrobacterium tumefaciens transfers single-stranded transferred DNA (T-DNA) into the plant cell nucleus. Proceedings of the National Academy of Sciences USA, 91, 8000-8004.
316. Tinland, B., Schoumacher, F., Gloeckler, V., Bravo-Angel, A.M. and Hohn, B. (1995). The Agrobacterium tumefaciens virulence D2 protein is responsible for precise integration of T-DNA into the plant genome. The EMBO Journal, 14, 3585-3595.
317. Topping, J.F., Wei, W. and Lindsey, K. (1991). Functional tagging of regulatory elements in the plant genome. Development, 112, 1009-1019.
318. Toro, N., Datta, A., Yanofsky, M. and Nester, E. (1988). Role of the overdrive sequence in T-DNA border cleavage in Agrobacterium. Proceedings of the National Academy of Sciences USA, 85, 8558-8562.
319. Toro, N., Datta, A., Carmi, O.A., Young, C, Prusti, R.K. and Nester, E.W. (1989). The Agrobacterium tumefaciens virCl gene product binds to overdrive, a T-DNA transfer enhancer. Journal of Bacteriology, 171, 6845-6849.
320. Turk, S.C.H.J., Melchers, L.S., den Dulk-Ras, H., Regensburg-Tuink, A.J.G. and Hooykaas, P.J.J. (1991). Environmental conditions differentially affect vir gene induction in different Agrobacterium strains. Role of the VirA sensor protein. Plant Molecular Biology, 16, 1051-1059.
321. Turk, S.C.H.J., Nester, E.W. and Hooykaas, P.J.J. (1993). The vir A promoter is a host-range determinant in Agrobacterium tumefaciens. Molecular Microbiology, 7, 719-724.
322. Uttaro, A.D., Cangelosi, G.A., Geremia, R.A., Nester, E.W. and Ugalde, R.A. (1990). Biochemical characterization of avirulent exoC mutants of Agrobacterium tumefaciens. Journal of Bacteriology, 172, 1640-1646.
323. Valvekens, D., Van Montagu, M. and Van Lijsebettens, M. (1988). Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root expiants by using kanamycin selection. Proceedings of the National Academy of Sciences USA, 85, 5536-5540.
324. van den Elzen, P.J.M., Townsend, J., Lee, K.Y. and Bedbrook, J.R. (1985). A chimaeric hygromycin resistance gene as a selectable marker in plant cells. Plant Molecular Biology, 5, 299-302.
325. van der Graaff, E., den Dulk-Ras, A. and Hooykaas, P.J.J. (1996). Deviating T-DNA transfer from Agrobacterium tumefaciens to plants. Plant Molecular Biology, 31, 677-681.
326. van Wordragen, M.F. and Dons, H.J.M. (1992). Agrobactenum tumefaciens-mediated transformation of recalcitrant crops. Plant Molecular Biology Reponer, 10, 12-36.
327. Van den Ackerveken, G, Marois, E. and Bonas, U. (1996). Recognition of the bacterial avirulence protein AvrBs3 occurs inside the host plant cell. Cell, 87, 1307-1316.
328. Van Haaren, M.J.J., Pronk, J.T., Schilperoort, R.A. and Hooykaas, P.J.J. (1987a) Functional analysis of the Agrobacterium tumefaciens octopine Ti-plasmid left and right T-region border fragments. Plant Molecular Biology, 8, 95-104.
329. Van Haaren, M.J.J., Sedee, N.J.A., Schilperoort, R.A. and Hooykaas, P.J.J. (1987b). Overdrive is a T-region transfer enhancer which stimulates T-strand production in Agrobacterium tumefaciens. Nucleic Acids Research, 15, 8983-8997.
330. Van Haaren, M.J.J., Sedee, N.J.A., Krul, M., Schilperoort, R.A. and Hooykaas, P.J.J. (1988). Function of heterologous and pseudo border repeats in T region transfer via the octopine virulence system of Agrobacterium tumefaciens. Plant Molecular Biology, 11, 773-781.
331. Van Haute, E., Joos, H., Maes, M., Warren, G, Van Montagu, M. and Schell, J. (1983). Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of Ti plasmids of Agrobacterium tumefaciens. The EMBO Journal, 2, 411-418.
332. Van Lijsebettens, M., Inzé, D., Schell, J. and Van Montagu, M. (1986). Transformed cell clones as a tool to study T-DNA integration mediated by Agrobacterium tumefaciens. Journal of Molecular Biology, 188, 129-145.
333. Van Lijsebettens, M., Vanderhaeghen, R., De Block, M., Bauw, G, Villarroel, R. and Van Montagu, M. (1994). An SI 8 ribosomal protein gene copy, encoded at the Arabidopsis PFL locus, affects plant development by its specific expression in meristems. The EMBO Journal, 13, 3378-3388.
334. Vaudequin-Dransart, V., Petit, A., Poncet, C, Ponsonnet, C, Nesme, X., Jones, J.B., Bouzar, H., Chilton, W.S. and Dessaux, Y. (1995). Novel Ti plasmids in Agrobacterium strains isolated from fig tree and chrysanthemum tumors and their opinelike molecules. Molecular Plant-Microbe Interactions, 8, 311-321.
335. Vogel, A.M. and Das, A. (1992). Mutational analysis of Agrobacterium tumefaciens virDl: tyrosine 29 is essential for endonuclease activity. Journal of Bacteriology, 174, 303-308.
336. Vogel, A.M., Yoon, J. and Das, A. (1995). Mutational analysis of a conserved motif of Agrobacterium tumefaciens VirD2. Nucleic Acids Research, 23, 4087-4091.
337. Wabiko, H. and Minemura, M. (1996). Exogenous phyto-hormone-independent growth and regeneration of tobacco plants transgenic for the 6b gene of Agrobactenum tumefaciens AKE10. Plant Physiology, 112, 939-951.
338. Wagner, V.T. and Matthysse, A.G. (1992). Involvement of a vitronectin-like protein in attachment of Agrobacterium tumefaciens to carrot suspension culture cells. Journal of Bacteriology, 174, 5999-6003.
339. Waldron, C, Murphy, E.B., Roberts, J.L., Gustafson, G.D., Armour, S.L. and Malcolm, S.K. (1985). Resistance to hygromycin B: a new marker for plant transformation studies. Plant Molecular Biology, 5, 103-108.
340. Wallroth, M., Gerats, A.G.M., Rogers, S.G., Fraley, R.T. and Horsch, R.B. (1986). Chromosomal localization of foreign genes in Petunia hybrida. Molecular and General Genetics, 202, 6-15.
341. Wang, K., Herrera-Estrella, L., Van Montagu, M. and Zambryski, P. (1984). Right 25-bp terminus sequences of the nopaline T-DNA is essential for and determines direction of DNA transfer from Agrobacterium to the plant genome. Cell, 38, 455-462.
342. Wang, K., Stachel, S., Timmerman, B., Van Montagu, M. and Zambryski, P. (1987a). Site-specific nick in the T-DNA border sequence following vir gene expression in Agrobacterium. Science, 235, 587-591.
343. Wang, K., Genetello, C, Van Montagu, M. and Zambryski, P. (1987b). Sequence context of the T-DNA border repeat element determines its relative activity during T-DNA transfer to plant cells. Molecular and General Genetics, 210, 338-346.
344. Wang, K., Herrera-Estrella, A. and Van Montagu, M. (1990). Overexpression of virDl and virD2 genes in Agrobacterium tumefaciens enhances T-complex formation and plant transformation. Journal of Bacteriology, 172, 4432-4440.
345. Wang, J., Lewis, M.E., Whallon, J.H. and Sink, K.C. (1995). Chromosomal mapping of T-DNA inserts in transgenic Petunia by in situ hybridization. Transgenic Research, 4, 241-246.
346. Ward, E.R. and Barnes, W.M. (1988). VirD2 protein of Agrobactenum tumefaciens very tightly linked to the 5' end of T-strand DNA. Science, 242, 927-930.
347. Ward, J.E., Akiyoshi, D.E., Régnier, D., Datta, A., Gordon, M.P. and Nester, E.W. (1988). Characterization of the virB operon from an Agrobacterium tumefaciens Ti plasmid. Journal of Biological Chemistry, 263, 5804-5814 (Erratum Journal of Biological Chemistry, 265, p. 4768).
348. Waters, V.L. and Guiney, D.G. (1993). Processes at the nick region link conjugation, T-DNA transfer and rolling circle replication. Molecular Microbiology, 9, 1123-1130.
349. Waters, V.L., Hirata, K.H., Pansegrau, W., Lanka, E. and Guiney, D.G. (1991). Sequence identity in the nick regions of IncP plasmid transfer origins and T-DNA borders of Agrobacterium Ti plasmids. Proceedings of the National Academy of Sciences USA, 88, 1456-1460.
350. Weising, K. and Kahl, G (1996). Natural genetic engineering of plant cells: the molecular biology of crown gall and hairy root disease. World Journal of Microbiology and Biotechnology, 12, 327-351.
351. White, F.F. and Nester, E.W. (1980). Relationship of plasmids responsible for hairy root and crown gall tumorigenicity. Journal of Bacteriology, 144, 710-720.
352. Winans, S.C., Ebert, P.R., Stachel, S.E., Gordon, M.P. and Nester, E.W. (1986). A gene essential for Agrobacterium virulence is homologous to a family of positive regulatory loci. Proceedings of the National Academy of Sciences USA, 83, 8278-8282.
353. Winans, S.C, Kerstetter, R.A. and Nester, E.W. (1988). Transcriptional regulation of the vir A and virG genes of Agrobacterium tumefaciens. Journal of Bacteriology, 170, 4047-4054.
354. Winans, S.C, Kerstetter, R.A., Ward, J.E. and Nester, E.W. (1989). A protein required for transcriptional regulation of Agrobacterium virulence genes spans the cytoplasmic membrane. Journal of Bacteriology, 171, 1616-1622.
355. Winans, S.C, Burns, D.L. and Christie, P.J. (1996). Adaptation of a conjugal transfer system for the export of pathogenic macromolecules. Trends in Microbiology, 4, 64-68.
356. Wirawan, I.G.P. and Kojima, M. (1996). A chromosomal virulence gene (acvB) product of Agrobacterium tumefaciens that binds to a T-strand to mediate its transfer to host plant cells. Biosciences, Biotechnology and Biochemistry, 60, 44-49.
357. Wirawan, I.G.P., Kang, H.W. and Kojima, M. (1993). Isolation and characterization of a new chromosomal virulence gene of Agrobacterium tumefaciens. Journal of Bacteriology, 175, 3208-3212.
358. Yadav, N.S., Vanderleyden, J., Bennett, D.R., Barnes, W.M. and Chilton, M.-D. (1982). Short direct repeats flank the T-DNA on a nopaline Ti plasmid. Proceedings of the National Academy of Sciences USA, 79, 6322-6326.
359. Yamada, T., Lee, P.-D. and Kosuge, T. (1986). Insertion sequence elements of Pseudomonas savastanoi: nucleotide sequence and homology with Agrobacterium tumefaciens transfer DNA. Proceedings of the National Academy of Sciences USA, 83, 8263-8267.
360. Yanofsky, M.F., Porter, S.G., Young, C, Albright, L.M., Gordon, M.P. and Nester, E.W. (1986). The virD Operon of Agrobacteńum tumefaciens encodes a site-specific en-donuclease. Cell, 47, 471-477.
361. Yoshioka, Y., Takahashi, Y., Matsuoka, K., Nakamura, K., Koizumi, J., Kojima, M. and Machida, Y. (1996). Transient gene expression in plant cells mediated by Agrobacterium tumefaciens: application for the analysis of virulence loci. Plant Cell Physiology, 37, 782-789.
362. Young C. and Nester, E.W. (1988). Association of the VirD2 protein with the 5' end of T strands in Agrobacterium tumefaciens. Journal of Bacteriology, 170, 3367-3374.
363. Yusibov, V.M., Steck, T.R., Gupta, V. and Gelvin, S.B. (1994). Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells. Proceedings of the National Academy of Sciences USA, 91, 2994-2998.
364. Zaenen, I., Van Larebeke, N., Teuchy, H., Van Montagu, M. and Schell, J. (1974). Supercoiled circular DNA in crown gall inducing Agrobacterium strains. Journal of Molecular Biology, 86, 109-127.
365. Zambryski, P., Depicker, A., Kruger, K. and Goodman, H. (1982). Tumor induction of Agrobacterium tumefaciens: analysis of the boundaries of T-DNA. Journal of Molecular and Applied Genetics, 1, 361-370.
366. Zambryski, P., Joos, H., Genetello, C, Leemans, J., Van Montagu, M. and Schell, J. (1983). Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity. EMBO Journal, 2, 2143-2150.
367. Zambryski, P., Tempé, J. and Schell, J. (1989). Transfer and function of T-DNA genes from Agrobacterium Ti and Ri plasmids in plants. Cell, 56, 193-201.
368. Zhou, H., Arrowsmith, J.W., Fromm, M.E., Hironaka, CM., Taylor, M.L., Rodriguez, D., Pajeau, M.E., Brown, S.M., Santino, CG. and Fry, J.E. (1995). Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Reports, 15, 159-163.
369. Ziegelin, G, Pansegrau, W., Strack, B., Balzer, D., Kröger, M., Kruft, V. and Lanka, E. (1991). Nucleotide sequence and organization of genes flanking the transfer origin of promiscuous plasmid RP4. DNA Sequence, 1, 303-327.
370. Ziegelin, G., Pansegrau, W., Lurz, R. and Lanka, E. (1992). TraK protein of conjugative plasmid RP4 forms a specialized nucleoprotein complex with the transfer origin. Journal of Biological Chemistry, 267, 17279-17286.
371. Zorreguieta, A., Geremia, R.A., Cavaignac, S., Cangelosi, G.A., Nester, E.W. and Ugalde, R.A. (1988). Identification of the product of an Agrobacterium tumefaciens chromosomal virulence gene. Molecular Plant-Microbe Interactions, 1, 121-127.
372. Zupan, J.R. and Zambryski, P. (1995). Transfer of T-DNA from Agrobacterium to the plant cell. Plant Physiology, 107, 1041-1047.
373. Zupan, J.R., Citovsky, V. and Zambryski, P. (1996). Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells. Proceedings of the National Academy of Sciences USA, 93, 2392-2397.