Application of phage display peptide libray to autoimmune diabetes: Identification of IA-2/ICA512bdc dominant autoantigenic epitopes

European Journal of Immunology

Volumn 32, Issue 5, 2002, Pages 1420-1427

  Farilla, Loredana ^a   Tiberti, Claudio ^a   Luzzago, Alessandra ^b   Yu, Liping ^c   Eisenbarth, George S ^c   Cortese, Riccardo ^b   Dotta, Francesco ^a,d   Mario, Umberto Di ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b IRBM   (Italy)

^c UNIVERSITY OF COLORADO DENVER   (United States)

^d c/o: DEM Foundation ^*  (Italy)

Author keywords

Autoantibody; Autoimmunity; Epitope; Type 1 diabetes

Indexed keywords

AMINO ACID; AUTOANTIBODY; AUTOANTIGEN; CAPSID PROTEIN; CELL ANTIGEN; COMPLEMENTARY DNA; EPITOPE; HYBRID PROTEIN; PEPTIDE LIBRARY; PROTEIN IA 2; PROTEIN ICA512BDC; PROTEIN TYROSINE KINASE; UNCLASSIFIED DRUG;

ADOLESCENT; ADULT; ANTIBODY DETECTION; ARTICLE; AUTOIMMUNE DISEASE; BACTERIOPHAGE LAMBDA; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CHILD; CONTROLLED STUDY; DNA LIBRARY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; INSULIN DEPENDENT DIABETES MELLITUS; MAJOR CLINICAL STUDY; MALE; MOLECULAR CLONING; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN DOMAIN; RADIOIMMUNOPRECIPITATION; SCREENING TEST; SEQUENCE ANALYSIS;

ADOLESCENT; AUTOANTIBODIES; AUTOANTIGENS; BASE SEQUENCE; BINDING, COMPETITIVE; CHILD; DIABETES MELLITUS, TYPE 1; DNA, COMPLEMENTARY; FEMALE; HUMANS; IMMUNODOMINANT EPITOPES; MALE; MEMBRANE PROTEINS; PEPTIDE LIBRARY; PROTEIN-TYROSINE-PHOSPHATASE;

EID: 0036106442     PISSN: 00142980     EISSN: None     Source Type: Journal    
DOI: 10.1002/1521-4141(200205)32:5<1420::AID-IMMU1420>3.0.CO;2-B     Document Type: Article

References (21)

1. Insulin-dependent diabetes mellitus
2. ICA 512, an autoantigen of type 1 diabetes, is an intrinsic protein of neurosecretory granules
3. Cloning and expression of IDDM-specific human antigens
4. Molecular cloning and identification of a receptor-type protein tyrosine phosphatase, IA-2, from human insulinoma
5. Molecular cloning of phogrin, a protein-tyrosine phosphatase homologue localized to insulin secretory granule membranes
6. Evaluation of islet cell antigen (ICA) 512/IA-2 autoantibody radioassays using overlapping ICA512/IA-2 constructs
7. ICA 512 autoantibody radioassay
8. T cell mediated autoimmunity to the IA-2 islet tyrosine phosphatase in type 1 diabetes mellitus
9. Autoantibodies to IA-2 in IDDM. Location of major antigenic determinants
10. Definition of multiple ICA512/phogrin autoantibody epitopes and detection of intramolecular epitope spreading in relatives of patients with type 1 diabetes mellitus
11. Early development and spreading of autoantibodies to epitopes of IA-2 and their association with progression to type 1 diabetes
12. Display of peptide and proteins on the surface of bacteriophage λ
13. Surface display of proteins on bacteriophage λ heads
14. Efficient display of an HCV cDNA expression library as C-terminal fusion to the capsid protein D of bacteriophage lambda
15. Use of tagged random hexamer amplification (TRHA) to clone and sequence minute quantities of DNA-application to a 180 kb plasmid isolated from Sphingomonas F199
16. Bacteriophage lambda display of complex cDNA libraries: A new approach to functional genomics
17. Mapping of novel autoreactive epitopes of the diabetes-associated autoantigen IA-2
18. Human monoclonal antibodies isolated from type 1 diabetes patients define multiple epitopes in the protein tyrosine phosphatase-like IA-2 antigen
19. Prediction of type 1 diabetes in first-degree relatives using a combination of insulin, GAD and ICA512bdc/IA-2 autoantibodies
20. Phage-displayed peptides as tools for the characterization of human sera
21. A novel radioligand binding assay to determine diagnostic accuracy of isoform specific glutamic acid decarboxylase in a childhood populations