Studying cytoskeletal dynamics in living cells using green fluorescent protein

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 21, Issue 3, 2002, Pages 241-250

  Yoon, Yisang ^a   Pitts, Kelly ^a   McNiven, Mark ^a  

^a MAYO CLINIC   (United States)

Author keywords

Cytoskeleton; Dynamics; GFP; Living cells; Motors

Indexed keywords

GREEN FLUORESCENT PROTEIN; CYTOSKELETON PROTEIN; HYBRID PROTEIN; PHOTOPROTEIN;

CELL SHAPE; CELL STRUCTURE; CYTOSKELETON; GENE EXPRESSION REGULATION; INTERMEDIATE FILAMENT; INTRACELLULAR TRANSPORT; MICROFILAMENT; MICROTUBULE; MOLECULAR DYNAMICS; MOLECULAR STABILITY; MORPHOGENESIS; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BINDING; REVIEW; RIGIDITY; ANIMAL; CELL LINE; DICTYOSTELIUM; GENETICS; HAMSTER; METABOLISM; RAT;

ANIMAL; CELL LINE; CYTOSKELETAL PROTEINS; CYTOSKELETON; DICTYOSTELIUM; HAMSTERS; LUMINESCENT PROTEINS; RATS; RECOMBINANT FUSION PROTEINS; ANIMALS; CRICETINAE; GREEN FLUORESCENT PROTEINS;

EID: 0036095118     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:21:3:241     Document Type: Review

References (59)

1. The green fluorescent protein
2. GFP variants for multispectral imaging of living cells
3. Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescence lifetime imaging microscopy
4. Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein
5. Visualization of Pit-1 transcription factor interactions in the living cell nucleus by fluorescence resonance energy transfer microscopy
6. Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer
7. PRIM: Proximity imaging of green fluorescent protein-tagged polypeptides
8. Microfilament dynamics during cell movement and chemotaxis monitored using a GFP-actin fusion protein
9. The suitability and application of a GFP-actin fusion protein for long-term imaging of the organization and dynamics of the cytoskeleton in mammalian cells
10. Movement of yeast cortical actin cytoskeleton visualized in vivo
11. Actin dynamics in lamellipodia of migrating border cells in the Drosophila ovary revealed by a GFP-actin fusion
12. Rapid actin-based plasticity in dendritic spines
13. Actin dynamics in living mammalian cells
14. Direct observations of the mechanical behaviors of the cytoskeleton in living fibroblasts
15. Listeria monocytogenes moves rapidly through the host-cell cytoplasm by inducing directional actin assembly
16. Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite. Listeria monocytogenes
17. Listeria monocytogenes exploits normal host cell processes to spread from cell to cell
18. Dynamics of actin-based movement by Rickettsia rickettsii in vero cells
19. Motile properties of vimentin intermediate filament networks in living cells
20. Rapid movements of vimentin on microtubule tracks: Kinesin-dependent assembly of intermediate filament networks
21. Insights into the dynamic properties of keratin intermediate filaments in living epithelial cells
22. Fate of the inner nuclear membrane protein lamin B receptor and nuclear lamins in herpes simplex virus type 1 infection
23. Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex
24. Mitosis in living budding yeast: Anaphase A but no metaphase plate
25. Autoregulated instability of tubulin mRNAs: A novel eukaryotic regulatory mechanism
26. GFP illuminates the cytoskeleton
27. Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein-alpha tubulin
28. Unconventional myosins in cell movement, membrane traffic, and signal transduction
29. Myosin dynamics in live Dictyostelium cells
30. Myosin II dynamics and cortical flow during contractile ring formation in Dictyostelium cells
31. Cytokinesis failure in clathrinminus cells is caused by cleavage furrow instability
32. A role for myosin VII in dynamic cell adhesion
33. Green fluorescent protein marks skeletal muscle in murine cell lines and zebrafish
34. Kinesin and dynein superfamily proteins and the mechanism of organelle transport
35. Kinesin and dynein superfamily proteins in organelle transport and cell division
36. The Kar3p and Kip2p motors function antagonistically at the spindle poles to influence cytoplasmic microtubule numbers
37. The kinesin-related proteins, Kip2p and Kip3p, function differently in nuclear migration in yeast
38. Centrosome and spindle function of the Drosophila Ncd microtubule motor visualized in live embryos using Ncd-GFP fusion proteins
39. Direct visualization of the movement of the monomeric axonal transport motor UNC-104 along neuronal processes in living Caenorhabditis elegans
40. Cilia and flagella of eukaryotes
41. Microtubule-associated protein 1C from brain is a two-headed cytosolic dynein
42. DYNEINS: Molecular structure and cellular function
43. A novel cytoplasmic dynein heavy chain: Expression of DHC1b in mammalian ciliated epithelial cells
44. Mammalian cells express three distinct dynein heavy chains that are localized to different cytoplasmic organelles
45. Digital time-lapsed DIC/Fluorescence imaging of dynein-GFP reveals dynamics of astral microtubules in Saccharomyces cerevisiae throughout the cell cycle
46. Astral microtubule dynamics in yeast: A microtubule-based searching mechanism for spindle orientation and nuclear migration into the bud
47. Dynamics of cytoplasmic dynein in living cells and the effect of a mutation in the dynactin complex actin-related protein Arp1
48. Dynamin: A molecular motor with pinchase action
49. Molecular cloning of the microtubule-associated mechanochemical enzyme dynamin reveals homology with a new family of GTP-binding proteins
50. Identification of dynamin, a novel mechanochemical enzyme that mediates interactions between microtubules
51. Identification of dynamin 2, an isoform ubiquitously expressed in rat tissues
52. Differential expression and regulation of multiple dynamins
53. A novel member of the dynamin family of GTP-binding proteins is expressed specifically in the testis
54. Three dynamin-encoding genes are differentially expressed in developing rat brain
55. Differential distribution of dynamin isoforms in mammalian cells
56. ER-to-Golgi transport visualized in living cells
57. Swing of the lever arm of a myosin motor at the isomerization and phosphate-release steps
58. Single molecular assay of individual ATP turnover by a myosin-GFP fusion protein expressed in vitro
59. Role of the kinesin neck region in processive microtubule-based motility