The suitability and application of a GFP-actin fusion protein for long-term imaging of the organization and dynamics of the cytoskeleton in mammalian cells

European Journal of Cell Biology

Volumn 77, Issue 2, 1998, Pages 81-90

  Choidas, Axel ^a   Jungbluth, Andreas ^a   Sechi, Antonio ^b   Murphy, John ^a   Ullrich, Axel ^a   Marriott, Gerard ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Cytoskeleton; Fluorescence microscopy; GFP actin; Motility

Indexed keywords

ACTIN; F ACTIN; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; NERVE GROWTH FACTOR; PHALLOIDIN;

ANIMAL CELL; ARTICLE; BLADDER CARCINOMA; BLEACHING; CELL STRAIN 3T3; CONTROLLED STUDY; CYTOSKELETON; DNA TRANSFECTION; FLUORESCENCE; HELA CELL; HUMAN; HUMAN CELL; LAMELLIPODIUM; LISTERIA MONOCYTOGENES; MAMMAL CELL; MESENCHYME; NONHUMAN; PRIORITY JOURNAL;

EID: 0031792970     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0171-9335(98)80075-7     Document Type: Article

References (32)

1. Boom, R., C. J. Sol, R. P. Minnaar, J. L. Geelen, A. K. Raap, J. van der Noordaa: Induction of gene expression under human cytomegalovirus immediate early enhancer-promoter control by inhibition of protein synthesis is cell cycle-dependent. J. Gen. Virol. 69, 1179-1193 (1988).
2. Boyer, B., G. C. Tucker, A. M. Valles, W. W. Franke, J. P. Thiery: Rearrangements of desmosomal and cytoskeletal proteins during the transition from epithelial to fibroblastoid organization in cultured rat bladder carcinoma cells. J. Cell Biol. 109, 1495-1509 (1989).
3. Button, E., C. Shapland, D. Lawson: Actin, its associated proteins and metastasis. Cell Motil. Cytoskeleton 30, 247-251 (1995).
4. Clarke, M. S., P. L. McNeil: Syringe loading introduces macromolecules into living mammalian cell cytosol. J. Cell Sci. 102, 533-541 (1992).
5. Cole, N. B., C. L. Smith, N. Sciaky, M. Terasaki, M. Edidin, J. Lippincott-Schwartz: Diffusional mobility of Golgi proteins in membranes of living cells. Science 273, 797-801 (1996).
6. Conrad, P. A., M. A. Nederlof, I. M. Herman, D. L. Taylor: Correlated distribution of actin, myosin, and microtubules at the leading edge of migrating Swiss 3T3 fibroblasts. Cell Motil. Cytoskeleton 14, 527-543 (1989).
7. Cudmore, S., I. Reckmann, G. Griffiths, M. Way: Vaccinia virus: a model system for actin-membrane interactions. J. Cell Sci. 109, 1739-1747 (1996).
8. Davis, L. G., M. D. Dibnar, J. F. Battey: In: Basic Methods in Molecular Biology. pp. 286-289. Elsevier Science Publishing Co., New York 1986.
9. Doyle, T., D. Botstein: Movement of yeast cortical actin cytoskeleton visualized in vivo. Proc. Natl. Acad. Sci. USA 93, 3886-3891 (1996).
10. Fischer, M., S. Kaech, D. Knutti, A. Matus: A rapid actin-based plasticity in dendritic spines. Neuron 20, 847-854 (1998).
11. Haugwitz, M., A. A.Noegel, J. Karakesisoglou, M.Schleicher: Dictyostelium amoebae that lack G-actin-sequestering profilins show defects in F-actin content, cytokinesis, and development. Cell 79, 303-314 (1994).
12. Heidecker, M., Y. Yan-Marriott, G. Marriott: Proximity relationships and structural dynamics of the phalloidin-binding site of actin filaments in solution and on single actin filaments on heavy meromyosin. Biochemistry 34, 11017-11024 (1995).
13. Hoock, T. C., P. M. Newcomb, I.M.Herman: Beta-actin and its mRNA are localized at the plasma membrane and the regions of moving cytoplasm during the cellular response to injury. J. Cell Biol. 112, 653-664 (1991).
14. Li, J., C.Yen, D.Liaw, K. Podsypanina, S. Bose, S.I. Wang, J. Puc, C. Miliaresis, L. Rodgers, R. McCombie, S. H. Bigner, B.C. Giovanella, M. Ittmann, B. Tycko, H. Hibshoosh, M. H. Wigler, R. Parsons: PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science 275, 1943-1947 (1997).
15. Liao, J. C., L. J. Berg, D. G. Jay: Chromophore-assisted laser inactivation of subunits of the T-cell receptor in living cells is spatially restricted. Photochem. Photobiol. 62, 923-929 (1995).
16. McKenna, N., J. B.Meigs, Y.-L. Wang: Identical distribution of fluorescently labeled brain and muscle actins in living cardiac fibroblasts and myocytes. J. Cell Biol. 100, 292-296 (1983).
17. Ormo, M., A. B. Cubitt, K. Kallio, L. A. Gross, R. Y Tsien, S. J. Remington: Crystal structure of the Aequorea victoria green fluorescent protein. Science 273, 1392-1395 (1996).
18. Prassler, J., S. Stocker, G. Marriott, M. Heidecker, J. Kellermann, G. Gerisch: Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes. Mol. Biol. Cell 8, 83-95 (1997).
19. Rosenblatt, J., B. J. Agnew, H. Abe, J. R. Bamburg, T. J. Mitchison: Xenopus actin depolytnerizing factor/cofilin (XAC) is responsible for the turnover of actin filaments in Listeria monocytogenes tails. J. Cell Biol. 136, 1323-1332 (1997).
20. Sanders, M. C., Y. L. Wang: Assembly of actin-containing cortex occurs at distal regions of growing neurites in PC12 cells. J. Cell Sci. 100, 771-780 (1991).
21. Sechi, A. S., J. Wehland, J. V. Small: The isolated comet tail pseudopodium of Listeria monocytogenes: a tail of two actin filament populations, long and axial and short and random. J. Cell Biol. 137, 155-167 (1997).
22. Symons, M. H., T. J. Mitchison: Control of actin polymerization in live and permeabilized fibroblasts. J. Cell Biol. 114, 503-513 (1991).
23. Tanaka, J., H.Sadanari, H. Sato, S.Fukuda: Sodium butyrateinducible replication of human cytomegalovirus in a human epithelial cell line. Virology 185, 271-280 (1991).
24. Taylor, D. L., Y. L. Wang: Molecular cytochemistry: incorporation of fluorescently labeled actin into living cells. Proc. Natl. Acad. Sci. USA 75, 857-861 (1978).
25. Theriot, J. A., T. J. Mitchison, L. G. Tilney, D. A. Portnoy: The rate of actin-based motility of intracellular Listeria monocytogenes equals the rate of actin polymerization. Nature 357, 257-260 (1992).
26. Theriot, J. A., T. J. Mitchison: Actin microfilament dynamics in locomoting cells. Nature 352, 126-131 (1991).
27. Tilney, L. G., D. A. Portnoy: Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes, J. Cell Biol. 109, 1597-1608 (1989).
28. Valles, A. M., B. Boyer, J. Badet, G. C. Tucker, D. Barritault, J. P. Thiery: Acidic fibroblast growth factor is a modulator of epithelial plasticity in a rat bladder carcinoma cell line. Proc. Natl. Acad. Sci. USA 87, 1124-1128 (1990).
29. Verkhovsky, A. B., T. M. Svitkina, G. G. Borisy: Myosin II filament assemblies in the active lamella of fibroblasts: their morphogenesis and role in the formation of actin filament bundles. J. Cell Biol. 131, 989-1002 (1995).
30. Vigers, G. P., M. Coue, J. R. Mclntosh: Fluorescent microtubules break up under illumination. J. Cell Biol. 107, 1011-1024 (1988).
31. Wang, Y. L.: Exchange of actin subunits at the leading edge of living fibroblasts: possible role of treadmilling. J. Cell Biol. 101, 597-602(1985).
32. Westphal, M., A. Jungbluth, M. Heidecker, B. Mühlbauer, C. Heizer, J.-M. Schwartz, G. Marriott, G. Gerisch: Microfilament dynamics during cell movement and chemotaxis monitored using a GFP-actin fusion protein. Curr. Biol. 7, 176-183 (1997).