Quantitative monitoring of the mRNA expression pattern of the TGF-β-isoforms (β1, β2, β3) during transdifferentiation of hepatic stellate cells using a newly developed real-time SYBR Green PCR

Biochemical and Biophysical Research Communications

Volumn 295, Issue 2, 2002, Pages 330-335

  Wickert, L ^a   Steinkruger S ^a   Abiaka, M ^a   Bolkenius, U ^a   Purps, O ^a   Schnabel, C ^a   Gressner, A M ^a  

^a UNIVERSITY HOSPITAL RWTH AACHEN   (Germany)

Author keywords

Hepatic stellate cells; mRNA quantification; Real time PCR; TGF isoforms

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; ISOPROTEIN; MESSENGER RNA; TRANSFORMING GROWTH FACTOR BETA;

ACCURACY; ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CELL ACTIVATION; CELL DIFFERENTIATION; CONTROLLED STUDY; GENE SEQUENCE; HOUSEKEEPING GENE; LIVER CELL; MALE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RAT; REPRODUCIBILITY; STELLATE CELL;

ANIMALS; BASE SEQUENCE; CELL DIFFERENTIATION; DNA PRIMERS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HEPATOCYTES; MALE; POLYMERASE CHAIN REACTION; PROTEIN ISOFORMS; RATS; RATS, SPRAGUE-DAWLEY; REFERENCE STANDARDS; RNA, MESSENGER; TRANSFORMING GROWTH FACTOR BETA;

EID: 0036068852     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(02)00669-1     Document Type: Article

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