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Volumn 352, Issue , 2002, Pages 3-31

Measurement of absolute oxygen levels in cells and tissues using oxygen sensors and 2-nitroimidazole EF5

Author keywords

[No Author keywords available]

Indexed keywords

1 (1,1,1,3,3,3 HEXAFLUORO 2 PROPOXY) 3 (2 NITRO 1 IMIDAZOLYL) 2 PROPANOL; 1 FLUORO 3 (2 NITRO 1 IMIDAZOLYL) 2 PROPANOL; 2 (2 NITRO 1H IMIDAZOL 1 YL) N (2,2,3,3,3 PENTAFLUOROPROPYL)ACETAMIDE; 2 NITROIMIDAZOLE; ASCORBIC ACID; CYTOCHROME C OXIDASE; EF 3; ETANIDAZOLE; GLUCOSE; HETEROCYCLIC COMPOUND; MISONIDAZOLE; MONOCLONAL ANTIBODY; NITROGEN DERIVATIVE; OXYGEN; PIMONIDAZOLE; POLYACRYLAMIDE; THEOPHYLLINE; UNCLASSIFIED DRUG; 2-(2-NITRO-1H-IMIDAZOL-1-YL)-N-(2,2,3,3,3-PENTAFLUOROPROPYL)ACETAMIDE; DRUG DERIVATIVE; FLUORINATED HYDROCARBON;

EID: 0036048395     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(02)52003-6     Document Type: Article
Times cited : (173)

References (90)
  • 3
  • 29
    • 85030741774 scopus 로고    scopus 로고
    • note
    • The solubility of gas is temperature dependent, as is the partial pressure due to water vapor. In this report, the following assumptions ale made regarding oxygen solubility. At 37°, the partial pressure of water vapor is ∼45 mm of Hg; thus, atmospheric pressure of 760 mm of Hg of air with saturated water vapor allows for oxygen (21%) + nitrogen (79%) partial pressures of 149 + 566 mm of Hg, respectively. The solubility of oxygen in physiological saline (or equivalent) equilibrated with water vapor-saturated air at 37° is about 0.20 mM. At 25° and 4°, the corresponding solubility is about 0.25 and 0.45 mM, respectively. In the text, we report absolute concentrations (in micromolar) and mm of Hg or percentage oxygen values assuming a temperature of 37°.
  • 31
    • 85030739413 scopus 로고    scopus 로고
    • "Polarographic Oxygen Sensor with Glass Seal." Canada Patent, 1304449: June 30, 1992
    • C. J. Koch, "Polarographic Oxygen Sensor with Glass Seal." Canada Patent, 1304449: June 30, 1992.
    • Koch, C.J.1
  • 33
    • 85030740345 scopus 로고    scopus 로고
    • note
    • Polycarbonate is the most oxygen permeable of plastics made for tissue culture use (dishes are made by NUNC). The least toxic and water-impermeable tubing is made from silicone, but it is by far the worst in the sense of high oxygen permeability. It is not the present intention to make a blanket condemnation of experiments using plastics. Rather, it should be appreciated that many oxygen-dependent chemical and biological phenomena are very sensitive to the range of oxygen below 2% (some much lower still). Thus, use of plastics may prevent a proper understanding of the oxygen dependence of various biological phenomena.
  • 37
    • 85030748456 scopus 로고
    • Institute of Physics, London
    • C. J. Koch, in "Proc. 6th L. H. Gray Conf. (1974)," p. 167. Institute of Physics, London, 1975.
    • (1974) Proc. 6th L. H. Gray Conf. , pp. 167
    • Koch, C.J.1
  • 38
    • 85030743567 scopus 로고    scopus 로고
    • note
    • To promote cell attachment, glass is cleaned, heated to 500°, and then conditioned by overnight treatment with water containing 50 mM sodium carbonate and 15% serum, followed by 1 hr with water containing tissue culture grade gelatin (0.1%). The gelatin is removed, but the thin remaining film is allowed to dry onto the glass under UV light in a sterile hood.
  • 43
    • 85030746647 scopus 로고    scopus 로고
    • note
    • It is important to realize that the internal volume of gas regulators is several tens of milliliters. Thus, when a regulator is first connected to a gas cylinder (or pressurized after disuse), this internal oxygen-containing volume is highly pressurized by the cylinder gas (e.g., nitrogen) and can cause a long-lasting contamination of oxygen if used at low flow rates. To prevent this, the regulator must be purged by a series of on-off cycles of the high-pressure cylinder valve, each followed by release of the gas from the regulator.
  • 59
    • 0343047075 scopus 로고
    • G. E. Adams, A. Breccia, E. M. Fielden, and P. Wardman, eds. Plenum Press, NY
    • C. J. Koch, in "Selective Activation of Drugs by Redox Processes" (G. E. Adams, A. Breccia, E. M. Fielden, and P. Wardman, eds.), p. 237. Plenum Press, NY, 1990.
    • (1990) Selective Activation of Drugs by Redox Processes , pp. 237
    • Koch, C.J.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.