Function in Escherichia coli of the non-catalytic part of RNase E: Role in the degradation of ribosome-free mRNA

Molecular Microbiology

Volumn 45, Issue 5, 2002, Pages 1231-1243

  Leroy, Anne ^a   Vanzo, Nathalie F ^a,c   Sousa, Sandra ^b,d   Dreyfus, Marc ^b   Carpousis, Agamemnon J ^a  

^a UMR   (France)

^b ECOLE NORMALE SUPÉRIEURE   (France)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^d INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; ENOLASE; MESSENGER RNA; MUTANT PROTEIN; POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE; RIBONUCLEASE E; RNA HELICASE; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; AUTOREGULATION; BACTERIAL GENE; BACTERIAL GROWTH; COMPETITIVE ABILITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME STABILITY; ESCHERICHIA COLI; GENE DELETION; GENETIC CODE; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; REPORTER GENE; RIBOSOME; RNA BINDING; RNA DEGRADATION; RNA TRANSCRIPTION; RNA TRANSLATION;

ENDORIBONUCLEASES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENES, BACTERIAL; GENES, REPORTER; LAC OPERON; RNA STABILITY; RNA, BACTERIAL; RNA, MESSENGER; SEQUENCE DELETION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0036047092     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2002.03104.x     Document Type: Article

References (51)

1. Copurification of E. coli RNAase E and PNPase: Evidence for a specific association between two enzymes important in RNA processing and degradation
2. mRNA degradation. A tale of poly(A) and multiprotein machines
3. Cloning and analysis of the entire Escherichia coli ams gene. Ams is identical to hmp1 and encodes a 114 kDa protein that migrates as a 180 kDa protein
4. Degradation of mRNA in Escherichia coli: An old problem with some new twists
5. Reconstitution of a minimal RNA degradosome demonstrates functional coordination between a-3′ exonuclease and a DEAD-box RNA helicase
6. Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: Mature 5S rRNA is dispensable for ribosome function
7. Structural requirements for the processing of Escherichia coli 5S ribosomal RNA by RNase E in vitro
8. RNase E activity is conferred by a single polypeptide: Over-expression, purification, and properties of the ams/rne/hmp1 gene product
9. Restriction of the activity of the recombination site dif to a small zone of the Escherichia coli chromosome
10. Dob1p (Mtr4p) is a putative ATP-dependent RNA helicase required for the 3′ end formation of 5.8S rRNA in Saccharomyces cerevisiae
11. Exoribonucleases and their multiple roles in RNA metabolism
12. An evolutionarily conserved RNA stem-loop functions as a sensor that directs feedback regulation of RNase E gene expression
13. Genetic and biochemical approaches for analysis of mitochondrial degradosome from Saccharomyces cerevisiae
14. Specificity of Escherichia coli endoribonuclease RNase E: in vivo and in vitro analysis of mutants in a bacteriophage T4 mRNA processing site
15. Structural analysis and in vitro processing to p5 rRNA of a 9S RNA molecule isolated from an rne mutant of E. coli
16. Messenger RNA stability and its role in control of gene expression in bacteria and phages
17. The stability of Escherichia coli lacZ mRNA depends upon the simultaneity of its synthesis and translation
18. The 3′ to 5′ degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3′ to 5′ exonucleases of the exosome complex
19. An mRNA degrading complex in Rhodobacter capsulatus
20. RNase E autoregulates its synthesis by controlling the degradation rate of its own mRNA in Escherichia coli: Unusual sensitivity of the rne transcript to RNase E activity
21. Consequences of RNase E scarcity in Escherichia coli
22. Regions of RNase E important for 5′-end-dependent RNA cleavage and autoregulated synthesis
23. The endoribonucleolytic N-terminal half of Escherichia coli RNase E is evolutionarily conserved in Synechocystis sp. and other bacteria but not the C-terminal half, which is sufficient for degradosome assembly
24. RNase E polypeptides lacking a carboxyl-terminal half suppress a mukB mutation in Escherichia coli
25. RNase G (CafA protein) and RNase E are both required for the 5′ maturation of 16S ribosomal RNA
26. Effects of nucleotide sequence on the specificity of rne-dependent and RNase E-mediated cleavages of RNA I encoded by the pBR322 plasmid
27. RNA degradosomes exist in vivo in Escherichia coli as multicomponent complexes associated with the cytoplasmic membrane via the N-terminal region of ribonuclease E
28. The use of a tRNA as a transcriptional reporter: The T7 late promoter is extremely efficient in Escherichia coli but its transcripts are poorly expressed
29. The C-terminal half of RNase E, which organizes the Escherichia coli degradosome, participates in mRNA degradation but not rRNA processing in vivo
30. The N-terminal domain of the rne gene product has RNase E activity and is non-overlapping with the arginine-rich RNA-binding site
31. Transcribing of Escherichia coli genes with mutant T7 RNA polymerases: Stability of lacZ mRNA inversely correlates with polymerase speed
32. The DExH box protein Suv3p is a component of a yeast mitochondrial 3′- to-5′ exoribonuclease that suppresses group I intron toxicity
33. Proteins associated with RNase E in a multicomponent ribonucleolytic complex
34. RNase E, an RNA processing enzyme from Escherichia coli
35. Escherichia coli endoribonuclease RNase E: Autoregulation of expression and site-specific cleavage of mRNA
36. Escherichia coli RNase E has a role in the decay of bacteriophage T4 mRNA
37. The relationship between translational control and mRNA degradation for the Escherichia coli threonyl-tRNA synthetase gene
38. Analysis of mRNA decay and rRNA processing in Escherichia coli in the absence of RNase E-based degradosome assembly
39. A protein complex mediating mRNA degradation in Escherichia coli
40. A DEAD-box RNA helicase in the Escherichia coli RNA degradosome
41. Degradation of mRNA in bacteria: Emergence of ubiquitous features
42. D-E-A-D protein family of putative RNA helicases
43. Autoregulation allows Escherichia coli RNase E to adjust continuously its synthesis to that of its substrates
44. SYPRO orange and SYPRO red protein gel stains: One-step fluorescent staining of denaturing gels for detection of nanogram levels of protein
45. Use of T7 RNA polymerase to direct expression of cloned genes
46. Running rings around RNA: A superfamily of phosphate-dependent RNases
47. Evidence for an RNA binding region in the Escherichia coli processing endoribonuclease RNase E
48. The CafA protein required for the 5′-maturation of 16 S rRNA is a 5′-end-dependent ribonuclease that has context-dependent broad sequence specificity
49. Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome