Functional behavior of primary rat liver cells in a three-dimensional perfused microarray bioreactor

Tissue Engineering

Volumn 8, Issue 3, 2002, Pages 499-513

  Powers, Mark J ^a,b   Janigian, Dena M ^a   Wack, Kathryn E ^a   Baker, Carolyn S ^a,c   Stolz, Donna Beer ^d   Griffith, Linda G ^a  

^a USA   (United States)

^b BioWhittaker   (United States)

^c Genetics Institute   (United States)

^d UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALBUMIN; ALBUMINOID; DNA; UREA;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL AGGREGATION; CELL CULTURE; CELL FUNCTION; CELL ISOLATION; CELL METABOLISM; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; LIVER CELL; LIVER PERFUSION; MALE; METABOLIC RATE; NONHUMAN; PRIORITY JOURNAL; RAT; UREA CYCLE; ANIMAL; CYTOLOGY; ELECTRON MICROSCOPY; METABOLISM; MULTICELLULAR SPHEROID; PHYSIOLOGY; SECRETION; TIME; TISSUE ENGINEERING;

ALBUMINS; ANIMAL; BIOREACTORS; CELLS, CULTURED; DNA; HEPATOCYTES; MICROSCOPY, ELECTRON; RATS; SPHEROIDS; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.; TIME FACTORS; TISSUE ENGINEERING; UREA; ANIMALS; SPHEROIDS, CELLULAR;

EID: 0036019367     PISSN: 10763279     EISSN: None     Source Type: Journal    
DOI: 10.1089/107632702760184745     Document Type: Article

References (42)

1. Liver
2. Development of a small-scale bioreactor for drug metabolism studies maintaining hepatospecific functions
3. Numerical model of fluid flow and oxygen transport in a radial-flow microchannel containing hepatocytes
4. Primary hepatocytes outperform HepG2 cells as the source of biotransformation functions in a bioartificial liver
5. Improved hepatocyte in-vitro maintenance in a culture model with woven multicompartment capillary systems-electron microscopy studies
6. Entrapment of hepatocyte spheroids in a hollow fiber bioreactor as a potential bioartificial liver
7. Morphogenetic events in mixed cultures of rat hepatocytes and non-parenchymal cells maintained in biological matrices in the presence of hepatocyte growth factor and epidermal growth factor
8. Survival and function of rat hepatocytes cocultured with nonparenchymal cells or sinusoidal endothelial cells on biodegradable polymers under flow conditions
9. Controlling cell interactions by micropatterning in co-cultures: Hepatocytes and 3T3 fibroblasts
10. 3-D coculture of hepatic sinusoidal cells with primary hepatocytes-design of an organotypic model
11. Spheroidal aggregate culture of rat liver cells: Histotypic reorganization, biomatrix deposition, and maintenance of functional activities
12. Morphological and immuno-cytochemical characterization of a hetero-spheroid composed of fibroblasts and hepatocytes
13. Preservation and inducibility of xenobiotic metabolism in long-term cultures of adult rat liver cell aggregates
14. Adhesion-guided in vitro morphogenesis in pure and mixed cell cultures
15. Reconstruction of a hepatic organoid by rat small hepatocytes and hepatic nonparenchymal cells
16. A microarray perfusion bioreactor for 3D liver culture
17. Control of hepatocyte function on collagen foams: Sizing matrix pores toward selective induction of 2-D and 3-D cellular morphogenesis
18. Preparation of isolated rat liver cells
19. Population expansion, clonal growth, and specific differentiation patterns in primary cultures of hepatocytes induced by HGF/SF, EGF and TGFα in a chemically defined (HGM) medium
20. Efficient assembly of rat hepatocyte spheroids for tissue engineering applications
21. DNA fluorometric assay in 96-well tissue culture plates using Hoechst 33258 after cell lysis by freezing in distilled water
22. ELISA for determination of albumin in the nanogram range: Assay in cerebrospinal fluid and comparison with radial immunodiffusion
23. Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration
24. Packed-bed type reactor to attain high density culture of hepatocytes for use as a bioartificial liver
25. Hepatocyte behavior within three-dimensional porous alginate scaffolds
26. Amino acid inhibition of the autophagic/lysosomal pathway of protein degradation in isolated rat hepatocytes
27. Amino acid and energy requirements for rat hepatocytes in primary culture
28. Amino acid control of autophagic sequestration and protein degradation in isolated rat hepatocytes
29. Strategies for restoration and maintenance of normal hepatic structure and function in long-term cultures of rat hepatocytes
30. Glutamine or glutamate release by the liver constitutes a major mechanism for nitrogen salvage
31. Encapsulated multicellular spheroids of rat hepatocytes produce albumin and urea in a spouted bed circulating culture system
32. Rapid hepatocyte spheroid formation: Optimization and long term function in perfused microcapsules
33. Hepatic tissue engineering: Development of critical technologies
34. A novel bioreactor design for in vitro reconstruction of in vivo liver characteristics
35. Microencapsulated hepatocytes. Prospects for extracorporeal liver support
36. Reconstruction of liver tissue in vitro: Geometry of characteristic flat bed, hollow fiber, and spouted bed bioreactors with reference to the in vivo liver
37. Continued high albumin production by multicellular spheroids of adult rat hepatocytes formed in the presence of liver-derived proteoglycans
38. Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments
39. Long-term culture of rat liver cell spheroids in hormonally defined media
40. Importance of cell aggregation for expression of liver functions and regeneration demonstrated with primary cultured hepatocytes
41. Kinetics of hepatocyte spheroid formation
42. Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids