Identification of Escherichia coli O157:H7 and other enterohemorrhagic serotypes by EHEC-hlyA targeting, strand displacement amplification, and fluorescence polarization

Molecular and Cellular Probes

Volumn 16, Issue 2, 2002, Pages 85-92

  Ge, B ^a   Larkin, C ^a   Ahn, S ^a   Jolley, M ^a   Nasir, M ^a   Meng, J ^a   Hall, R H ^a  

^a UNIVERSITY OF MARYLAND   (United States)

Author keywords

Enterohemorrhagic E. coli; Fluorescence polarization; Rapid detection; Strand displacement amplification

Indexed keywords

HEMOLYSIN; BACTERIAL TOXIN; CYTOTOXIN; EHEC HLYA PROTEIN, E COLI; EHEC-HLYA PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN;

AMPLICON; ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; DISEASE CONTROL; ESCHERICHIA COLI; FLUORESCENCE POLARIZATION; FOOD CONTAMINATION; GENE TARGETING; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; SEROTYPE; STATISTICAL ANALYSIS; STRAIN DIFFERENCE; WATER CONTAMINATION; ANIMAL; CATTLE; CLASSIFICATION; ESCHERICHIA COLI O157; EVALUATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; NUCLEIC ACID AMPLIFICATION; SENSITIVITY AND SPECIFICITY; SEROTYPING;

ANIMALIA; BOVINAE; ESCHERICHIA COLI; HUMAN ECHOVIRUS 1;

ANIMALS; BACTERIAL TOXINS; BACTERIOLOGICAL TECHNIQUES; CATTLE; CYTOTOXINS; ESCHERICHIA COLI O157; ESCHERICHIA COLI PROTEINS; FLUORESCENCE POLARIZATION; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; SENSITIVITY AND SPECIFICITY; SEROTYPING; WATER MICROBIOLOGY;

EID: 0036018465     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.2001.0389     Document Type: Article

References (35)

1. Hemorrhagic colitis associated with a rare Escherichia coli serotype
2. Longitudinal study of Escherichia coli O157:H7 dissemination on four dairy farms in Wisconsin
3. A swimming-associated outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7 and Shigella sonnei
4. From the Centers for Disease Control and Prevention. Lake-associated outbreak of Escherichia coli O157:H7 - Illinois, 1995
5. Lake-associated outbreak of Escherichia coli O157:H7 - Illinois, 1995
6. Escherichia coli O157:H7 diarrhoea associated with well water and infected cattle on an Ontario farm
7. A waterborne outbreak in Missouri of Escherichia coli O157:H7 associated with bloody diarrhea and death
8. Swimming-associated haemorrhagic colitis due to Escherichia coli O157:H7 infection: Evidence of prolonged contamination of a fresh water lake
9. Enterohemorrhagic Escherichia coli in the United States
10. An epidemiological study on an outbreak of Escherichia coli O157:H7 infection
11. Massive outbreak of Escherichia coli O157:H7 infection in schoolchildren in Sakai City, Japan, associated with consumption of white radish sprouts
12. Food safety through the meat supply chain
13. The risks and prevention of contamination of beef feedlot cattle: The perspective of the United States of America
14. Produce handling and processing practices
15. Non-O157:H7 pathogenic Shiga toxin-producing Escherichia coli: Phenotypic and genetic profiling of virulence traits and evidence for clonality
16. The large-sized plasmids of enterohemorrhagic Escherichia coli O157 strains encode hemolysins which are presumably members of the E. coli alpha-hemolysin family
17. Virulence genes of Shiga toxin-producing Escherichia coli isolated from food, animals and humans
18. Epidemiology and diagnosis of Shiga toxin-producing Escherichia coli infections
19. Strand displacement amplification - An isothermal, in vitro DNA amplification technique
20. Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system
21. Empirical aspects of strand displacement amplification
22. Simultaneous strand displacement amplification and fluorescence polarization detection of Chlamydia trachomatis DNA
23. Detection of Mycobacterium tuberculosis DNA with thermophilic strand displacement amplification and fluorescence polarization
24. DNA detection by strand displacement amplification and fluorescence polarization with signal enhancement using a DNA binding protein
25. Strand displacement amplification (SDA) and transient-state fluorescence polarization detection of Mycobacterium tuberculosis DNA
26. Fluorescence polarization: An analytical tool for immunoassay and drug discovery
27. Escherichia coli O157:H7 and O157:H(-) strains that do not produce Shiga toxin: Phenotypic and genetic characterization of isolates associated with diarrhea and hemolytic-uremic syndrome
28. Enterohemolytic phenotypes and genotypes of shiga toxin-producing Escherichia coli O111 strains from patients with diarrhea and hemolytic-uremic syndrome
29. Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933
30. Analysis of the EHEC hly operon and its location in the physical map of the large plasmid of enterohaemorrhagic Escherichia coli O157:H7
31. A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome
32. Strand displacement amplification and homogeneous real-time detection incorporated in a second-generation DNA probe system, BDProbeTecET
33. A multiplex PCR for identifying Shiga-like toxin-producing Escherichia coli O157:H7