Intron-dependent stimulation of marker gene expression in cultured insect cells

Insect Molecular Biology

Volumn 11, Issue 1, 2002, Pages 87-95

  Zieler, H ^a,b   Huynh, C Q ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b Chromatin Inc   (United States)

Author keywords

Gene expression; Insect cells; Intron splicing; Luciferase

Indexed keywords

AEDES ALBOPICTUS; ANIMALIA; AUTOGRAPHA; AUTOGRAPHA CALIFORNICA; AUTOGRAPHA CALIFORNICA NUCLEOPOLYHEDROVIRUS; CYPOVIRUS; DROSOPHILA MELANOGASTER; INSECTA; MELANOGASTER; UNIDENTIFIED NUCLEAR POLYHEDROSIS VIRUSES; VECTORS;

COMPLEMENTARY DNA; LUCIFERASE;

AEDES; ANIMAL; ARTICLE; BINDING SITE; CELL LINE; CYTOLOGY; GENE; GENE EXPRESSION; GENE VECTOR; GENETICS; INTRON; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; REPORTER GENE; RNA SPLICING;

AEDES; ANIMALS; BASE SEQUENCE; BINDING SITES; CELL LINE; DNA, COMPLEMENTARY; GENE EXPRESSION; GENES, INSECT; GENES, REPORTER; GENETIC VECTORS; INTRONS; LUCIFERASES; MOLECULAR SEQUENCE DATA; RNA SPLICING;

EID: 0036008685     PISSN: 09621075     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0962-1075.2001.00312.x     Document Type: Article

References (78)

1. Insect cells as hosts for the expression of recombinant glycoproteins
2. The Aequoria victoria green fluorescent protein can be used as a reporter in live zebrafish embryos
3. Genetic transformation systems in insects
4. Transcriptional regulatory sequences within the first intron of the chicken apolipoproteinAl (apoAl) gene
5. Regulatory elements in the first intron contribute to transcriptional control of the human alpha 1 (l) collagen gene
6. Introns increase transcriptional efficiency in transgenic mice
7. Regulatory elements in the first intron contribute to transcriptional regulation of the beat 3 tubulin gene by 20-hydroxyecdysone in Drosophila Kc cells
8. Comparison of intron-dependent and intron-independent gene expression
9. Introns increase gene expression in cultured maize cells
10. Regulation by HIV Rev depends on recognition of splice sites
11. A generic intron increases gene expression in transgenic mice
12. Importance of introns for expression of mouse ribosomal protein gene rpL32
13. The yeast splicing factor Mud13p is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear cap-binding complex
14. The cap and 3′ splice site similarly affect polyadenytation efficiency
15. Advances in the use of recombinant baculoviruses for the expression of heterologous proteins
16. Introns boost transgene expression in Drosophila melanogaster
17. Introns in the 3′-untranslated region can inhibit chimeric CAT and beta-galactosidase gene expression
18. Participation of the nuclear cap binding complex in the pre-mRNA 3′ processing
19. Intron and upstream sequences regulate expression of the Drosophila beta3-tubulin gene in the visceral and somatic musculature, respectively
20. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene
21. Multiple factors are required for specific RNA cleavage at a poly (A) addition site
22. Characterization of the aldolase B intronic enhancer
23. Introns are inconsequential to efficient formation of cellular thymidine kinase mRNA in mouse L cells
24. Identification of a phenotype-specific enhancer in the first intron of the rat collagen II gene
25. Intervening sequences increase efficiency of RNA 3′ processing and accumulation of cytoplasmic RNA
26. The simian virus 40 small t intron, present in many common expression vectors, leads to aberrant splicing
27. The interplay between multiple enhancer and silencer elements defines the pattern of decapentaplegic expression
28. Construction of modular and versatile plasmid vectors for the high-level expression of single and multiple genes in insects and insect cell lines
29. A cap-binding protein complex mediating U snRNA export
30. A nuclear cap binding protein complex involved in pre-mRNA splicing
31. Transport of RNA between nucleus and cytoplasm
32. Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells
33. Continuous foreign gene expression in transformed lepidopteran insect cells
34. Immediate-early baculovirus vectors for foreign gene expression in transformed or infected insect cells
35. Baculovirus vectors for expression in insect cells
36. Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm
37. Recombinant baculoviruses as expression vectors for insect and mammalian cells
38. Optimizing expression of transgenes with an emphasis on post-transcriptional events
39. Pre-mRNA splicing alters RNP composition: Evidence for stable association of proteins at exon - Exon junctions
40. A yeast cap binding protein complex (yCBC) acts at an early step in pre-mRNA splicing
41. A nuclear cap-binding complex facilitates association of U1 snRNP with the cap-proximal 5′ splice site
42. HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression
43. Splicing is required for rapid and efficient mRNA export in metazoans
44. The combination of a novel stimulatory element in the first exon of the maize Shrunken-1 gene with the following intron 1 enhances reporter gene expression up to 1000-fold
45. Components of vectors for gene transfer and expression in mammalian cells
46. The insects are coming
47. Stable insect cell cultures for recombinant protein production
48. Identification and characterization of a testis-specific isoform of a chaperonin in a moth, Heliothis virescens
49. The intron requirement for immunoglobulin gene expression is dependent upon the promoter
50. Mutation of the AAUAAA polyadenylation signal depresses in vitro splicing of proximal but not distal introns
51. In vitro polyadenylation is stimulated by the presence of an upstream intron
52. Recent developments in transgenic insect technology
53. Preferential excision of the 5′ proximal intron from mRNA precursors with two introns as mediated by the cap structure
54. Splicing-independent expression of the herpes simplex virus type 1 thymidine kinase gene is mediated by three cis-acting RNA subelements
55. Importance of introns in the growth regulation of mRNA levels of the proliferating cell nuclear antigen gene
56. Heterologous introns can enhance expression of transgenes in mice
57. Silencing of the expression of the immunoglobulin kappa gene in non-B cells
58. Green fluorescent protein as a genetic marker in transgenic Aeries aegypti
59. The HIV-1 Rev protein
60. Effects of promoter, intron and enhancer elements on transient gene expression in sugar-cane and carrot protoplasts
61. Intron-mediated enhancement of transgene expression in maize is a nuclear, gene-dependent process
62. Baculovirus expression protocols
63. Regulatory elements in the 5′-flanking region and the first intron contribute to the transcriptional control of the mouse alpha 1 type I collagen gene
64. Simian virus 40 late transcripts lacking excisable intervening sequences are defective in both stability in the nucleus and transport to the cytoplasm
65. ICP27 mediates HSV RNA export by shuttling through a leucine-rich nuclear export signal and binding viral intronless RNAs through an RGG motif
66. The simian retrovirus-1 constitutive transport element, unlike the HIV-1 RRE, uses factors required for cellular mRNA export
67. Intron splicing and intron-mediated enhanced expression in monocots
68. A transcriptional silencer controls the developmental expression of the CD4 gene
69. A novel trancriptional suppressor located within a downstream intron of the BCR gene
70. A promoter within intron 35 of the human C4A gene initiates abundant adrenal-specific transcription of a 1 kb RNA: Location of a cryptic CYP21 promoter element?
71. The intron of the yeast actin gene contains the promoter for an antisense RNA
72. Increased gene expression by the first intron of maize Shrunken-1 locus in grass species
73. Novel downstream elements upregulate transcription initiated from an Epstein-Barr virus latent promoter
74. Intronic and flanking sequences are required to slilence enhancement of an embryonic beta-type globin gene
75. Targeting expression to the mammary gland: Intronic sequences can enhance the efficiency of gene expression in transgenic mice
76. Optimization of plasmid vectors for high-level expression in lung epithelial cells
77. The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans