Poly[Lys-(AEDTP)]: A cationic polymer that allows dissociation of pDNA/cationic polymer complexes in a reductive medium and enhances polyfection

Bioconjugate Chemistry

Volumn 13, Issue 1, 2002, Pages 76-82

  Pichon, Chantal ^a   LeCam, Eric ^b   Guérin, Brigitte ^a   Coulaud, Dominique ^b   Delain, Etienne ^b   Midoux, Patrick ^a  

^a CENTRE DE BIOPHYSIQUE MOLÉCULAIRE   (France)

^b INSTITUT GUSTAVE ROUSSY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

COVALENT BONDS; ELECTROPHORESIS; GENE EXPRESSION; GENE TRANSFER; MOLECULAR BIOLOGY; PEPTIDES; SULFUR COMPOUNDS;

AMINO GROUP; CATIONIC POLYMERS; CELL NUCLEUS; EXOGENOUS GENES; GENE DELIVERY SYSTEMS; GLUTHATHIONE; PLASMID DNA; POLYMER BASED; POLYMER COMPLEX; POLYPLEXES;

DNA;

CATION; DITHIOTHREITOL; DNA; DRUG CARRIER; DRUG DERIVATIVE; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; FLUORESCENT DYE; LUCIFERASE; LYSINE; POLY(LYS (AEDTP)); POLY(LYS-(AEDTP)); POLYMER; REDUCING AGENT; THIOREDOXIN;

ARTICLE; CELL LINE; CHEMISTRY; ELECTROCHEMISTRY; ELECTRON MICROSCOPY; GENETIC TRANSFECTION; GENETICS; HUMAN; METHODOLOGY; OXIDATION REDUCTION REACTION; PLASMID;

CATIONS; CELL LINE; DITHIOTHREITOL; DNA; DRUG CARRIERS; ELECTROCHEMISTRY; FLUORESCENT DYES; HUMANS; INDICATORS AND REAGENTS; LUCIFERASES; LYSINE; MICROSCOPY, ELECTRON; OXIDATION-REDUCTION; PLASMIDS; POLYMERS; REDUCING AGENTS; THIOREDOXIN; TRANSFECTION;

EID: 0036007592     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc015503o     Document Type: Article

References (36)

1. Polylysine-based transfection systems utilizing receptor-mediated delivery
2. Pharmaceutical perspectives of nonviral gene therapy
3. Gene-delivery systems using cationic polymers
4. Interpolyelectrolyte and block ionomer complexes for gene delivery: Physicochemical aspects
5. Enhanced resistance to nuclease degradation of nucleic acids complexed to asialoglycoprotein-polylysine carriers
6. Synthetic polymers for vectorial delivery of DNA: Characterization of polymer-DNA complexes by photon correlation spectroscopy and stability to nuclease degradation and disruption by polyanions in vitro
7. Glycosylated polylysine/DNA complexes: Gene transfer efficiency in relation with the size and the sugar substitution level of glycosylated polylysines and with the plasmid size
8. The reduction of the positive charges of polylysine by partial gluconoylation increases the transfection efficiency of polylysine/DNA complexes
9. Vector unpacking as a potential barrier for receptor-mediated polyplex gene delivery
10. Muramyl dipeptide bound to poly-L-lysine substituted with mannose and gluconoyl residues as macrophage activators
11. Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines
12. A novel T7 RNA polymerase autogene for efficient cytoplasmic expression of target genes
13. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoeschst 33258 stain
14. Firefly luciferase gene: Structure and expression in mammalian cells
15. The spreading of nucleic acids
16. Introduction of a disulfide bond into a cationic lipid enhances transgene expression of plasmid DNA
17. Use of dithiodiglycolic acid as a tether for cationic lipids decreases the cytotoxicity and increases transgene expression of plasmid DNA in vitro
18. Reducible cationic lipids for gene transfer
19. Lipoic acid-derived amphiphiles for redox-controlled DNA delivery
20. Self-assembly of DNA-polymer complexes using template polymerization
21. Metabolic instability of plasmid DNA in the cytosol: A potential barrier to gene transfer
22. A potent new class of reductively activated peptide gene delivery agents
23. Specific inhibition of hepatitis B viral gene expression in vitro by targeted antisense oligonucleotides
24. Inhibition of leukemia cell proliferation by receptor-mediated uptake of c-myc antisense oligodeoxynucleotides
25. Inhibition of leukemia cell proliferation by folic acid - Polylysine-mediated introduction of c-myb antisense oligodeoxynucleotides into HL60 cells
26. Enhanced biological activity of antisense oligonucleotides complexed with glycosylated poly-L-lysine
27. Histidylated oligolysines increase the transmembrane passage and the biological activity of antisense oligonucleotides
28. Intracellular distribution of microinjected antisense oligonucleotides
29. Rapid nuclear accumulation of injected oligodeoxyribonucleotides
30. Intracellular disposition and metabolism of fluorescently labeled unmodified and modified oligonucleotides microinjected into mammalian cells
31. Size-dependent DNA mobility in cytoplasm and nucleus
32. Induction of tumor immunity and cytotoxic T lymphocyte responses using dendritic cells transfected with messenger RNA amplified from tumor cells
33. Human dendritic cells transfected with RNA encoding prostate-specific antigen stimulate prostate-specific CTL responses in vitro
34. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
35. Efficient gene transfer by histidylated polylysine/pDNA complexes
36. Histidylated oligolysines increase the transmembrane passage and the biological activity of antisense oligonucleotides