Expression of ecdysteroid receptor and ultraspiracle from Chironomus tentans (insecta, diptera) in E. coli and purification in a functional state

Insect Biochemistry and Molecular Biology

Volumn 32, Issue 2, 2002, Pages 167-174

  Grebe, Marco ^a   Spindler Barth, Margarethe ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

DNA binding; Hormone; Insect; Ligand binding; Nuclear receptor

Indexed keywords

ACRYLIC ACID RESIN; CF1 PROTEIN; DNA; DNA BINDING PROTEIN; DNA BINDING PROTEINS; ECDYSTEROID; ECDYSTEROID RECEPTOR; ECDYSTERONE; HORMONE RECEPTOR; HYBRID PROTEIN; POLYACRYLAMIDE GELS; PONASTERONE A; STEROID RECEPTOR; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; DRUG DERIVATIVE;

AFFINITY CHROMATOGRAPHY; ANIMAL; ANIMAL TISSUE; CHIRONOMIDAE; CHIRONOMUS TENTANS; CONFERENCE PAPER; CONTROLLED STUDY; DNA BINDING; ELECTROPHORETIC MOBILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HORMONE RECEPTOR INTERACTION; LIGAND BINDING; METABOLISM; METHODOLOGY; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ISOLATION; PROTEIN PROCESSING; WESTERN BLOTTING; ARTICLE; ISOLATION AND PURIFICATION;

CHIRONOMUS TENTANS; DIPTERA; INSECTA;

ACRYLIC RESINS; ANIMAL; BLOTTING, WESTERN; CHIRONOMIDAE; CHROMATOGRAPHY, AFFINITY; DNA-BINDING PROTEINS; ECDYSTERONE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; RECEPTORS, STEROID; RECOMBINANT FUSION PROTEINS; SUPPORT, NON-U.S. GOV'T; TRANSCRIPTION FACTORS;

EID: 0036006616     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(01)00098-4     Document Type: Conference Paper

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