Expression of EcR and USP in Escherichia coli: Purification and functional studies

Archives of Insect Biochemistry and Physiology

Volumn 35, Issue 1-2, 1997, Pages 59-69

  Elke, C ^a   Vogtli M ^a   Rauch, P ^b   Spindler Barth, M ^b   Lezzi, M ^a  

^a ETH ZURICH   (Switzerland)

^b HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Chironomus; DNA binding; Ecdysteroid receptor; Ligand binding; Polytene chromosomes

Indexed keywords

CF1 PROTEIN, INSECT; DNA; DNA BINDING PROTEIN; ECDYSONE RECEPTOR; ECDYSTERONE; HORMONE; HYBRID PROTEIN; LIGAND; STEROID RECEPTOR; TRANSCRIPTION FACTOR;

ANIMAL; ARTICLE; CHIRONOMIDAE; DROSOPHILA MELANOGASTER; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR CLONING;

ANIMALS; CHIRONOMIDAE; CLONING, MOLECULAR; DNA; DNA-BINDING PROTEINS; DROSOPHILA MELANOGASTER; ECDYSTERONE; ESCHERICHIA COLI; INVERTEBRATE HORMONES; LIGANDS; RECEPTORS, STEROID; RECOMBINANT FUSION PROTEINS; TRANSCRIPTION FACTORS;

EID: 0030624725     PISSN: 07394462     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1520-6327(1997)35:1/2<59::aid-arch6>3.0.co;2-s     Document Type: Article

References (20)

1. Antoniewski C, Mugat B, Delbac F, Lepesant J-A (1996): Direct repeats bind the EcR/USP receptor and mediate ecdysteroid responses in Drosophila melanogaster. Mol Cell Biol 16:2977-2986.
2. Ausubel FM, Brent R, Kingston RE, Moor DD, Seidman JG, Smith JA, Struhl K (1992): Short Protocols in Molecular Biology. New York: Greene Publ. Assoc. and Wiley & Sons.
3. Beato M, Herrlich P, Schütz G (1995): Steroid hormone receptors: Many actors in search of a plot. Cell 83:851-857.
4. Frangioni JV, Neel BG (1993): Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins. Anal Biochem 210:179-187.
5. Guan KL, Dixon JE (1991): Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal Biochem 192:262-267.
6. Hakes DJ, Dixon JE (1992): New vectors for high level expression of recombinant proteins in bacteria. Anal Biochem 202:293-298.
7. Henrich VC, Sliter TJ, Lubahn DB, MacIntyre A, Gilbert LI (1990): A steroid/thyroid hormone receptor superfamily member in Drosophila melanogaster that shares extensive sequence similarity with a mammalian homologue. Nucleic Acids Res 18:4143-4148.
8. Imhof MO, Rusconi S, Lezzi M (1993): Cloning of a Chironomus tentans cDNA encoding a protein (cEcRH) homologous to the Drosophila melanogaster ecdysteroid receptor (dEcR). Insect Biochem Mol Biol 23:115-124.
9. Khoury Christianson AM, King DL, Hatzivassiliou E, Casas JE, Hallenbeck PL, Nikodem VM, Mitsialis SA, Kafatos FC (1992): DNA binding and heteromerization of the Drosophila transcription factor chorion factor 1/ultraspiracle. Proc Natl Acad Sci USA 89:11503-11507.
10. Koelle MR, Talbot WS, Segraves WA, Bender MT, Cherbas P, Hogness DS (1991): The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily. Cell 67:59-77.
11. Lezzi M (1996): Chromosome puffing: Supramolecular aspects of ecdysone action. In Gilbert LI, Tata JR, Atkinson BG (eds): Metamorphosis: Postembryonic Reprogramming of Gene Expression in Amphibian and Insect Cells. San Diego: Academic Press, pp 145-173.
12. Lezzi M, Robert M (1972): Chromosomes isolated from unfixed salivary glands of Chironomus. Res Prob Cell Differ 4:35-57.
13. Riddihough G, Pelham HRB (1987): An ecdysone response element in the Drosophila hsp27 promoter. EMBO J 6:3729-3734.
14. Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW (1990): Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185:60-89.
15. Stunnenberg HG (1993): Mechanisms of transactivation by retinoic acid receptors. BioEssays 15:309-315.
16. Turberg A, Spindler KD (1992): Properties of nuclear and cytosolic ecdysteroid receptors from an epithelial cell line from Chironomus tentans. J Insect Physiol 38:81-91.
17. Wegmann IS, Quack S, Spindler KD, Dorsch-Häsler K, Vögtli M, Lezzi M (1995): Immunological studies on the developmental and chromosomal distribution of ecdysteroid receptor protein in Chironomus tentans. Arch Insect Biochem Physiol 30:95-114.
18. Widmer RM, Lucchini R, Lezzi M, Meyer B, Sogo JM, Edström JE, Koller T (1984): Chromatin structure of a hyperactive secretory protein gene (in Balbiani ring 2) of Chironomus. EMBO J 3:1635-1641.
19. Yao T-P, Segraves WA, Oro AE, McKeown M, Evans RM (1992): Drosophila ultraspiracle modulates ecdysone receptor function via heterodimer formation. Cell 71:63-72.
20. Yao T-P, Forman BM, Jiang ZY, Cherbas L, Chen JD, McKeown M, Cherbas P, Evans RM (1993): Functional ecdysone receptor is the product of EcR and Ultraspiracle genes. Nature 366:476-479.